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Yorodumi- PDB-5ghw: Crystal structure of broad neutralizing antibody 10E8 with long e... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5ghw | ||||||
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Title | Crystal structure of broad neutralizing antibody 10E8 with long epitope bound | ||||||
Components |
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Keywords | IMMUNE SYSTEM / BROAD NEUTRALIZING ANTIBODY / RECOMBINANT FAB / EPITOPE / HIV-1 / VIRAL MEMBRANE | ||||||
Function / homology | Function and homology information virus-mediated perturbation of host defense response => GO:0019049 / : / Synthesis and processing of ENV and VPU / evasion of host immune response / Alpha-defensins / Dectin-2 family / Binding and entry of HIV virion / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / positive regulation of establishment of T cell polarity ...virus-mediated perturbation of host defense response => GO:0019049 / : / Synthesis and processing of ENV and VPU / evasion of host immune response / Alpha-defensins / Dectin-2 family / Binding and entry of HIV virion / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / positive regulation of establishment of T cell polarity / virus-mediated perturbation of host defense response / host cell endosome membrane / actin filament organization / Assembly Of The HIV Virion / Budding and maturation of HIV virion / clathrin-dependent endocytosis of virus by host cell / membrane => GO:0016020 / viral protein processing / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / structural molecule activity / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Human immunodeficiency virus 1 | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Caaveiro, J.M.M. / Rujas, E. / Morante, K. / Nieva, J.L. / Tsumoto, K. | ||||||
Citation | Journal: Sci Rep / Year: 2016 Title: Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface Authors: Rujas, E. / Caaveiro, J.M. / Partida-Hanon, A. / Gulzar, N. / Morante, K. / Apellaniz, B. / Garcia-Porras, M. / Bruix, M. / Tsumoto, K. / Scott, J.K. / Jimenez, M.A. / Nieva, J.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5ghw.cif.gz | 191 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5ghw.ent.gz | 150.6 KB | Display | PDB format |
PDBx/mmJSON format | 5ghw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gh/5ghw ftp://data.pdbj.org/pub/pdb/validation_reports/gh/5ghw | HTTPS FTP |
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-Related structure data
Related structure data | 2ncsC 2nctC 4g6fS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 25648.656 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) | ||||
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#2: Antibody | Mass: 23152.633 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) | ||||
#3: Protein/peptide | Mass: 4623.701 Da / Num. of mol.: 1 / Fragment: UNP residues 674-698 / Source method: obtained synthetically / Source: (synth.) Human immunodeficiency virus 1 / References: UniProt: G3DED6, UniProt: P04578*PLUS | ||||
#4: Chemical | ChemComp-PO4 / #5: Water | ChemComp-HOH / | Sequence details | AUTHORS STATE THAT FOR ENTITY 1, GGS RESIDUES AT N-TERMINUS ARE CLONING ARTIFACTS. FOR ENTITY 3, ...AUTHORS STATE THAT FOR ENTITY 1, GGS RESIDUES AT N-TERMINUS ARE CLONING ARTIFACTS. FOR ENTITY 3, THE FOUR LYSINE RESIDUES AT THE N-TERMINUS AND THE FIVE LYSINE RESIDUES AT THE C-TERMINUS ARE SOLUBILIZA | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.24 Å3/Da / Density % sol: 62 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 / Details: 2.5 mM DPC 50% MPD 200 mM phosphate 100 mM TRIS |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NW12A / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Mar 1, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→52.4 Å / Num. obs: 24652 / % possible obs: 90 % / Redundancy: 9.7 % / CC1/2: 0.971 / Rmerge(I) obs: 0.046 / Net I/σ(I): 34 |
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 7 % / Rmerge(I) obs: 0.202 / Mean I/σ(I) obs: 8 / % possible all: 56.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4g6f Resolution: 2.4→52.4 Å / Cor.coef. Fo:Fc: 0.935 / Cor.coef. Fo:Fc free: 0.906 / SU B: 11.564 / SU ML: 0.147 / Cross valid method: THROUGHOUT / ESU R: 0.321 / ESU R Free: 0.233 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 40.341 Å2
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Refinement step | Cycle: 1 / Resolution: 2.4→52.4 Å
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