Mass: 29693.645 Da / Num. of mol.: 2 / Fragment: ectodomain, UNP residues 45-300 / Mutation: Q49T, N100D, N164D, N219D, N209D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CD38 / Plasmid: pPICZalpha / Production host: Komagataella pastoris (fungus) / Strain (production host): X33 References: UniProt: P28907, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
#2: Antibody
nanobodyMU1053
Mass: 17062.939 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lama glama (llama) / Plasmid: pHEN2 / Production host: Escherichia coli (E. coli)
Resolution: 2.3→50 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.91 / WRfactor Rfree: 0.2326 / WRfactor Rwork: 0.2031 / FOM work R set: 0.8483 / SU B: 14.552 / SU ML: 0.153 / SU Rfree: 0.2129 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.213 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2313
2259
5 %
RANDOM
Rwork
0.198
-
-
-
obs
0.1996
42953
87.75 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK
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