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- PDB-5b1y: Crystal structure of NADPH bound carbonyl reductase from Aeropyru... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5b1y | ||||||
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Title | Crystal structure of NADPH bound carbonyl reductase from Aeropyrum pernix | ||||||
![]() | 3-oxoacyl-[acyl-carrier-protein] reductase | ||||||
![]() | OXIDOREDUCTASE / NAD(P)H-dependent carbonyl reductase / Aeropyrum pernix K1 / hyperthermophile / archaea | ||||||
Function / homology | ![]() : / : / 3-oxoacyl-[acyl-carrier-protein] reductase / 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity / nucleotide binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yoneda, K. / Sakuraba, H. / Fukuda, Y. / Araki, T. / Ohshima, T. | ||||||
![]() | ![]() Title: Catalytic properties and crystal structure of thermostable NAD(P)H-dependent carbonyl reductase from the hyperthermophilic archaeon Aeropyrum pernix K1. Authors: Fukuda, Y. / Sakuraba, H. / Araki, T. / Ohshima, T. / Yoneda, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 111 KB | Display | ![]() |
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PDB format | ![]() | 84 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 24.4 KB | Display | |
Data in CIF | ![]() | 32.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2uvdS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29081.311 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 Gene: fabG, APE_2503.1 / Plasmid: pET15b / Production host: ![]() ![]() References: UniProt: Q9Y8Y1, 3-oxoacyl-[acyl-carrier-protein] reductase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.47 Å3/Da / Density % sol: 64.5 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 2.0 M NaCl, 0.1 M acetate buffer / PH range: 4.5-4.6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Jun 19, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.09→50 Å / Num. obs: 45349 / % possible obs: 99.7 % / Redundancy: 15.9 % / Biso Wilson estimate: 22.9 Å2 / Rmerge(I) obs: 0.064 / Net I/σ(I): 10 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2UVD Resolution: 2.09→33.1 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso mean: 37.3 Å2 | |||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.09→33.1 Å
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