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- PDB-4lgp: Ricin A chain bound to camelid nanobody (VHH1) -

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Basic information

Entry
Database: PDB / ID: 4lgp
TitleRicin A chain bound to camelid nanobody (VHH1)
Components
  • Ricin
  • VHH1 camelid nanobody
KeywordsHYDROLASE/IMMUNE SYSTEM / Ribosome inhibiting protein 2 / Camelid antibody (VHH) / HYDROLASE-IMMUNE SYSTEM complex
Function / homology
Function and homology information


rRNA N-glycosylase / rRNA N-glycosylase activity / AMP binding / defense response / toxin activity / carbohydrate binding / killing of cells of another organism / negative regulation of translation
Similarity search - Function
Ricin (A Subunit), domain 2 / Ricin (A Subunit), domain 2 / Ricin (A subunit); domain 1 / Ricin (A subunit), domain 1 / Ricin-type beta-trefoil lectin domain / Ribosome-inactivating protein conserved site / Shiga/ricin ribosomal inactivating toxins active site signature. / Ribosome-inactivating protein type 1/2 / Ribosome-inactivating protein / Ribosome-inactivating protein, subdomain 1 ...Ricin (A Subunit), domain 2 / Ricin (A Subunit), domain 2 / Ricin (A subunit); domain 1 / Ricin (A subunit), domain 1 / Ricin-type beta-trefoil lectin domain / Ribosome-inactivating protein conserved site / Shiga/ricin ribosomal inactivating toxins active site signature. / Ribosome-inactivating protein type 1/2 / Ribosome-inactivating protein / Ribosome-inactivating protein, subdomain 1 / Ribosome-inactivating protein, subdomain 2 / Ribosome-inactivating protein superfamily / Ribosome inactivating protein / Ricin-type beta-trefoil / Lectin domain of ricin B chain profile. / Ricin B, lectin domain / Ricin B-like lectins / Few Secondary Structures / Irregular / Immunoglobulins / Immunoglobulin-like / Sandwich / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
Biological speciesRicinus communis (castor bean)
Vicugna pacos (alpaca)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.4 Å
AuthorsRudolph, M.J. / Cheung, J. / Franklin, M. / Burshteyn, F. / Cassidy, M. / Gary, E. / Mantis, N.
CitationJournal: J.Mol.Biol. / Year: 2014
Title: Crystal Structures of Ricin Toxin's Enzymatic Subunit (RTA) in Complex with Neutralizing and Non-Neutralizing Single-Chain Antibodies.
Authors: Rudolph, M.J. / Vance, D.J. / Cheung, J. / Franklin, M.C. / Burshteyn, F. / Cassidy, M.S. / Gary, E.N. / Herrera, C. / Shoemaker, C.B. / Mantis, N.J.
History
DepositionJun 28, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 11, 2014Provider: repository / Type: Initial release
Revision 1.1Jul 2, 2014Group: Database references
Revision 1.2Jul 30, 2014Group: Database references
Revision 1.3Aug 20, 2014Group: Database references
Revision 1.4Mar 15, 2017Group: Source and taxonomy
Revision 1.5Nov 15, 2017Group: Refinement description / Category: software
Item: _software.classification / _software.contact_author ..._software.classification / _software.contact_author / _software.contact_author_email / _software.date / _software.language / _software.location / _software.name / _software.type / _software.version

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ricin
B: VHH1 camelid nanobody
C: Ricin
D: VHH1 camelid nanobody
hetero molecules


Theoretical massNumber of molelcules
Total (without water)83,7576
Polymers83,6604
Non-polymers982
Water2,450136
1
A: Ricin
B: VHH1 camelid nanobody
hetero molecules


Theoretical massNumber of molelcules
Total (without water)41,9274
Polymers41,8302
Non-polymers982
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2110 Å2
ΔGint-13 kcal/mol
Surface area16840 Å2
MethodPISA
2
C: Ricin
D: VHH1 camelid nanobody


Theoretical massNumber of molelcules
Total (without water)41,8302
Polymers41,8302
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1770 Å2
ΔGint-8 kcal/mol
Surface area16580 Å2
MethodPISA
Unit cell
Length a, b, c (Å)90.168, 92.443, 93.317
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein Ricin / / Ricin A chain / rRNA N-glycosidase


Mass: 29293.072 Da / Num. of mol.: 2 / Fragment: UNP residues 36-296
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Ricinus communis (castor bean) / Plasmid: pUTA / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P02879, rRNA N-glycosylase
#2: Antibody VHH1 camelid nanobody


Mass: 12536.873 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vicugna pacos (alpaca) / Plasmid: MCSG7 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) Rosetta
#3: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#4: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C2H6O2
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 136 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.32 Å3/Da / Density % sol: 47.08 %
Crystal growTemperature: 293 K / Method: vapor diffusion / pH: 8.5
Details: 100 mM Bicine, 20 % PEG 6000, pH 8.5, VAPOR DIFFUSION, temperature 293K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1.08 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 21, 2013
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.08 Å / Relative weight: 1
ReflectionResolution: 2.09→50 Å / Num. all: 46600 / Num. obs: 44876 / % possible obs: 96.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 4.8 % / Rmerge(I) obs: 0.162 / Χ2: 1.255 / Net I/σ(I): 4.7
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allΧ2Diffraction-ID% possible all
2.09-2.1320.60315710.538169.4
2.13-2.162.30.54818840.536182.2
2.16-2.212.50.5620440.6189.1
2.21-2.252.70.52821510.615192.9
2.25-2.33.10.52822500.606197.6
2.3-2.353.60.54222580.584199.4
2.35-2.414.10.56522990.612199.9
2.41-2.484.60.56523200.6381100
2.48-2.555.10.53322880.6751100
2.55-2.635.50.46723330.722199.9
2.63-2.735.90.44723060.8041100
2.73-2.846.20.37623020.8581100
2.84-2.976.30.30923291.006199.9
2.97-3.126.20.25223261.216199.9
3.12-3.326.20.223461.4381100
3.32-3.576.10.15423271.719199.8
3.57-3.935.80.12423202.035199.4
3.93-4.55.50.09423692.277199.3
4.5-5.675.40.08223692.163198.7
5.67-505.10.06824842.388198.2

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Phasing

PhasingMethod: molecular replacement
Phasing MRRfactor: 48.45 / Model details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation2.5 Å46.22 Å
Translation2.5 Å46.22 Å

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Processing

Software
NameVersionClassificationNB
DENZOdata reduction
SCALEPACKdata scaling
PHASER2.1.4phasing
PHENIX1.7.1_743refinement
PDB_EXTRACT3.11data extraction
HKL-2000data collection
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.4→46.222 Å / Occupancy max: 1 / Occupancy min: 0.28 / FOM work R set: 0.7779 / SU ML: 0.51 / σ(F): 0 / Phase error: 28.54 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2805 1561 5.03 %RANDOM
Rwork0.2249 ---
obs0.2278 31027 99.59 %-
all-31154 --
Solvent computationShrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 18.18 Å2 / ksol: 0.303 e/Å3
Displacement parametersBiso max: 113.33 Å2 / Biso mean: 44.9795 Å2 / Biso min: 15.49 Å2
Baniso -1Baniso -2Baniso -3
1-2.409 Å20 Å2-0 Å2
2--1.7159 Å2-0 Å2
3----2.3577 Å2
Refinement stepCycle: LAST / Resolution: 2.4→46.222 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5889 0 5 136 6030
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0046040
X-RAY DIFFRACTIONf_angle_d0.9378209
X-RAY DIFFRACTIONf_chiral_restr0.059900
X-RAY DIFFRACTIONf_plane_restr0.0041077
X-RAY DIFFRACTIONf_dihedral_angle_d16.0472176
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 11

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.4-2.47750.36011380.298226472785100
2.4775-2.5660.31811450.263326502795100
2.566-2.66880.30061400.248426372777100
2.6688-2.79020.35791260.253926582784100
2.7902-2.93730.331530.256326542807100
2.9373-3.12130.33141400.24326732813100
3.1213-3.36220.27021300.225926722802100
3.3622-3.70040.25531470.210226932840100
3.7004-4.23560.24051420.18582670281299
4.2356-5.33510.22441390.18172706284599
5.3351-46.23010.28351610.24432806296799
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.84051.5004-1.39813.8689-0.46450.40391.0791-1.09980.31950.5417-0.6888-0.0429-0.17240.32540.43640.04270.36970.0133-0.17640.02120.2024-5.024314.004112.2976
28.2624-0.8923.98742.7157-1.02688.91940.25230.47880.958-0.7055-0.76550.3936-0.39610.75630.53010.33570.0111-0.0620.15130.04220.30611.454514.36821.161
35.16640.1233-4.03261.2564-0.08059.5537-0.077-0.78260.6489-0.19680.5514-0.0809-0.29391.0681-0.21480.4036-0.0410.02570.6022-0.05490.252214.26739.077412.4024
44.13081.7503-0.27121.3954-0.17342.34230.13540.10020.3889-0.3467-0.09650.2199-0.5762-0.2824-0.16240.32130.0503-0.03520.2125-0.03550.2239-5.76096.465610.0923
58.7341-0.68880.35137.95531.57316.59420.08950.2306-0.4390.4501-0.3226-0.06610.17040.34240.0290.2394-0.05350.00130.18480.04140.095-1.4195-1.889720.3766
60.9421.1878-0.12831.97830.1414.1671-0.10380.0405-0.2111-0.4507-0.23760.34220.0418-0.04140.11330.19270.0983-0.06570.1455-0.01180.1478-5.6073-5.5248.5851
74.32861.8117-1.12866.2025-2.0674.15880.072-0.11770.35590.31230.3051.0612-0.4004-0.6546-0.11020.2580.0706-0.03510.3443-0.08610.4091-15.62780.58875.0464
86.64114.8429-3.02885.222-2.3811.5281-0.2690.5696-0.1704-0.4560.0711-0.15810.0469-0.2464-0.00180.28580.1043-0.04420.3049-0.05970.1031-4.0543-1.03752.7396
95.7203-1.199-0.58772.9265-0.23682.9750.65730.91660.3897-0.9691-0.12030.0507-0.0349-0.7776-0.20330.71190.05520.05520.4021-0.13820.0716-0.73835.2852-9.5514
106.20950.60941.09463.96750.82066.34490.1866-0.1679-0.4983-0.0374-0.2208-0.60820.52370.27110.03360.2915-0.00450.05880.23320.04750.128217.5576-1.8101-0.7225
117.51951.92873.32732.60711.98265.41010.05890.8469-0.3744-0.08090.0578-0.0415-0.0140.3059-0.18130.5411-0.10230.07760.399-0.03990.168416.0163-3.0096-10.2555
129.09330.22721.40886.50860.51322.09640.1117-1.08970.27820.1303-0.2159-0.7343-0.21250.7184-0.13710.29980.04330.04710.48960.00790.32516.89275.15864.6196
130.93191.60410.88852.8910.79354.014-0.9693-0.00270.06420.14710.34980.64510.2346-0.3140.24670.33990.00160.14120.71960.24410.8256-17.8418-9.014327.75
147.1887-4.24451.43594.0997-0.45425.5901-0.7838-0.1649-0.09891.23990.2628-0.13250.4177-0.77070.35750.62640.08050.47820.58110.29480.5809-8.4695-22.279145.3651
151.6029-1.6617-0.66293.7104-3.01926.9432-0.16390.4279-0.4824-0.03050.85741.070.1416-0.5837-0.49150.351-0.27340.01670.44480.01360.4591-14.2088-14.258224.1774
168.3381.6340.45934.54563.31162.73640.1956-1.44510.50321.0843-0.35810.0704-1.0269-0.2960.02530.70180.03840.01450.3632-0.04660.2456-3.7062-7.606536.9043
175.6307-1.7206-0.16553.4745-3.78394.97660.1790.23750.054-0.3439-0.24770.1335-0.73320.42750.00330.53090.06690.04990.22480.00030.3159-1.8076-15.592424.6224
187.4093-0.17152.99966.2312-1.84298.17440.21310.866-0.8717-0.06560.2276-0.80080.54960.862-0.50240.37460.06760.07080.30710.04530.4759-2.3284-20.667830.7093
198.65862.2933-1.25752.6755-0.22845.7798-0.0481.2549-0.2802-0.1336-0.03130.15181.23380.12640.34120.46-0.05850.06830.35460.07020.4963-13.2397-20.322327.21
202.40411.4354-1.20924.2978-0.0650.94620.2927-0.28650.33791.0285-0.25780.3546-0.10430.342-0.21060.6515-0.12640.0620.33580.04230.228-4.7047-14.913839.8183
216.92332.8995-0.62047.78380.30368.191-0.0074-0.4304-0.20060.66950.7505-0.1573-0.9003-0.2864-0.73260.42870.0223-0.00260.30190.19280.4033-6.5819-4.179529.4674
224.0273-0.6085-1.91752.29131.26782.53790.0601-0.2795-0.02240.3951-0.01640.044-0.2740.27920.02231.15250.30680.54160.4688-0.03670.0576-8.9981-15.324645.0092
233.59360.026-0.82762.4567-1.2252.4774-0.31340.2909-0.25810.0056-0.0093-0.09520.08160.15890.30450.17710.0231-0.00750.2154-0.0990.372135.4815-30.736311.4923
245.98441.2866-2.13129.4056-2.89227.18450.089-0.3341-0.47930.53580.1794-0.32480.22070.2207-0.4440.12520.0125-0.06470.1915-0.00340.356436.3829-24.988423.0218
251.9149-0.0324-0.43784.52854.20947.62910.338-0.17580.53-0.67970.2752-0.5918-1.29630.8309-0.50650.3108-0.06320.12860.237-0.10260.405532.7381-11.012612.2463
262.82292.6937-0.4693.4231-0.91563.0911-0.2899-0.3097-0.3827-0.34760.2194-0.64430.33890.35960.1230.2020.03350.04820.2655-0.00970.232828.1583-30.781913.4224
275.70461.1347-0.94326.91310.07134.2053-0.36710.58680.2779-0.14030.13680.14430.0223-0.295-0.21150.35-0.13220.04440.27950.01990.062120.7989-25.10793.1174
285.63741.73750.28192.1142-1.56312.7536-0.21050.0315-0.5011-0.750.2756-0.60840.18020.13050.04130.2805-0.08690.10270.1814-0.05180.314720.3936-32.14073.7706
298.00923.53241.08054.28451.54722.92440.2914-0.3782-0.026-0.1016-0.3620.4099-0.1075-0.18180.19550.23850.0720.01030.3088-0.07940.160412.4221-27.756223.7213
304.56180.84092.17833.37220.99883.80540.1370.0878-0.9149-0.33610.1628-0.44080.22780.0303-0.37280.309-0.05240.03520.1946-0.01160.330421.1558-40.222317.6959
314.81284.34092.31014.81592.07041.80570.0157-0.23780.11540.30520.0464-0.0309-0.04630.0231-0.060.22530.03790.0480.2453-0.03250.193520.436-28.694420.5973
324.4805-0.56320.18847.5942-1.30671.7691-0.1434-0.6136-0.14230.41670.002-0.5361-0.1286-0.25030.06560.4036-0.0907-0.06370.26670.00510.209126.7211-26.832233.3188
333.12691.0796-1.35484.5584-1.21762.7832-0.22050.47250.55660.04780.11910.3114-0.687-0.2159-0.08170.27760.0337-0.0180.0496-0.0340.159421.5036-7.463424.9875
343.51982.8449-1.19697.5008-3.5953.12770.3773-0.10720.23010.58680.08150.493-0.59790.3342-0.46040.3236-0.03350.03990.3378-0.1130.271319.2981-9.307834.116
355.07621.26191.28814.1328-0.52395.94470.22450.05440.7002-0.4606-0.22080.1501-0.3071-0.9874-0.14880.24670.1174-0.07010.304-0.05890.403928.7238-8.427520.1483
364.4529-0.12522.36083.57190.74655.38680.4242-0.4507-0.62030.4279-0.0538-1.0320.7313-0.0088-0.40540.6071-0.6279-0.05980.3018-0.15190.521311.2708-39.5004-6.6068
375.3902-2.0536-0.82212.3903-0.96061.14160.43550.6557-0.0715-1.0177-0.2340.25690.1321-0.44810.05040.6863-0.1344-0.10340.6412-0.2690.48031.2656-29.1969-23.3413
381.9724-1.36541.81481.99310.48974.2380.3539-0.5199-0.55360.6342-0.05240.61891.0591-0.3758-0.11510.4362-0.45580.09090.4862-0.00770.27657.1127-36.2183-1.8412
396.61470.162-1.0122.8768-0.53697.724-0.16930.85460.3697-0.27420.2731-0.31580.19851.1251-0.070.3101-0.03420.01810.6025-0.06820.232115.5155-26.254-13.6266
402.4297-0.02851.03463.72552.15287.9802-0.312-0.26270.38720.0930.33330.301-1.14780.01410.10530.32550.0476-0.05280.5149-0.15660.23337.314-23.7163-1.7373
414.9055-0.301-0.20953.7301-3.94937.61150.5636-0.40271.13180.1777-0.79040.4556-0.0435-0.39570.14680.30860.0083-0.00380.4458-0.24620.4182.7355-23.3512-8.4615
424.16633.3114-2.18156.4467-3.66362.11830.2987-0.3308-0.33730.04260.8181.12660.7249-1.447-1.07280.3998-0.2371-0.0280.504-0.03110.52931.6137-34.3048-5.3577
435.6432-1.25552.13291.9602-0.35182.6657-0.170.33380.43110.2706-0.04680.0111-0.4631-0.4780.33760.3655-0.14880.04280.5772-0.00520.28468.5151-26.4314-17.2035
446.89861.22811.02467.98460.3392.27250.29330.662-0.1764-0.89520.09070.17690.00941.1868-0.35840.39020.048-0.0330.5042-0.03860.140718.2644-29.48-6.2959
453.4159-0.7244-2.41483.22042.51383.27820.1339-0.34440.0219-0.2917-0.25660.0742-0.20460.0675-0.23610.39060.32090.15940.8555-0.349-0.54638.0794-30.6688-22.4848
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1CHAIN A AND (RESSEQ 1:17)A1 - 17
2X-RAY DIFFRACTION2CHAIN A AND (RESSEQ 18:32)A18 - 32
3X-RAY DIFFRACTION3CHAIN A AND (RESSEQ 33:56)A33 - 56
4X-RAY DIFFRACTION4CHAIN A AND (RESSEQ 57:86)A57 - 86
5X-RAY DIFFRACTION5CHAIN A AND (RESSEQ 87:106)A87 - 106
6X-RAY DIFFRACTION6CHAIN A AND (RESSEQ 107:140)A107 - 140
7X-RAY DIFFRACTION7CHAIN A AND (RESSEQ 141:160)A141 - 160
8X-RAY DIFFRACTION8CHAIN A AND (RESSEQ 161:180)A161 - 180
9X-RAY DIFFRACTION9CHAIN A AND (RESSEQ 181:201)A181 - 201
10X-RAY DIFFRACTION10CHAIN A AND (RESSEQ 202:229)A202 - 229
11X-RAY DIFFRACTION11CHAIN A AND (RESSEQ 230:248)A230 - 248
12X-RAY DIFFRACTION12CHAIN A AND (RESSEQ 249:261)A249 - 261
13X-RAY DIFFRACTION13CHAIN B AND (RESSEQ 1:7)B1 - 7
14X-RAY DIFFRACTION14CHAIN B AND (RESSEQ 8:17)B8 - 17
15X-RAY DIFFRACTION15CHAIN B AND (RESSEQ 18:33)B18 - 33
16X-RAY DIFFRACTION16CHAIN B AND (RESSEQ 34:45)B34 - 45
17X-RAY DIFFRACTION17CHAIN B AND (RESSEQ 46:61)B46 - 61
18X-RAY DIFFRACTION18CHAIN B AND (RESSEQ 62:73)B62 - 73
19X-RAY DIFFRACTION19CHAIN B AND (RESSEQ 74:83)B74 - 83
20X-RAY DIFFRACTION20CHAIN B AND (RESSEQ 84:97)B84 - 97
21X-RAY DIFFRACTION21CHAIN B AND (RESSEQ 98:110)B98 - 110
22X-RAY DIFFRACTION22CHAIN B AND (RESSEQ 111:116)B111 - 116
23X-RAY DIFFRACTION23CHAIN C AND (RESSEQ 1:17)C1 - 17
24X-RAY DIFFRACTION24CHAIN C AND (RESSEQ 18:32)C18 - 32
25X-RAY DIFFRACTION25CHAIN C AND (RESSEQ 33:56)C33 - 56
26X-RAY DIFFRACTION26CHAIN C AND (RESSEQ 57:86)C57 - 86
27X-RAY DIFFRACTION27CHAIN C AND (RESSEQ 87:106)C87 - 106
28X-RAY DIFFRACTION28CHAIN C AND (RESSEQ 107:122)C107 - 122
29X-RAY DIFFRACTION29CHAIN C AND (RESSEQ 123:140)C123 - 140
30X-RAY DIFFRACTION30CHAIN C AND (RESSEQ 141:160)C141 - 160
31X-RAY DIFFRACTION31CHAIN C AND (RESSEQ 161:180)C161 - 180
32X-RAY DIFFRACTION32CHAIN C AND (RESSEQ 181:201)C181 - 201
33X-RAY DIFFRACTION33CHAIN C AND (RESSEQ 202:229)C202 - 229
34X-RAY DIFFRACTION34CHAIN C AND (RESSEQ 230:248)C230 - 248
35X-RAY DIFFRACTION35CHAIN C AND (RESSEQ 249:261)C249 - 261
36X-RAY DIFFRACTION36CHAIN D AND (RESSEQ 2:7)D2 - 7
37X-RAY DIFFRACTION37CHAIN D AND (RESSEQ 8:17)D8 - 17
38X-RAY DIFFRACTION38CHAIN D AND (RESSEQ 18:33)D18 - 33
39X-RAY DIFFRACTION39CHAIN D AND (RESSEQ 34:45)D34 - 45
40X-RAY DIFFRACTION40CHAIN D AND (RESSEQ 46:61)D46 - 61
41X-RAY DIFFRACTION41CHAIN D AND (RESSEQ 62:73)D62 - 73
42X-RAY DIFFRACTION42CHAIN D AND (RESSEQ 74:83)D74 - 83
43X-RAY DIFFRACTION43CHAIN D AND (RESSEQ 84:97)D84 - 97
44X-RAY DIFFRACTION44CHAIN D AND (RESSEQ 98:110)D98 - 110
45X-RAY DIFFRACTION45CHAIN D AND (RESSEQ 111:116)D111 - 116

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