Mass: 18.015 Da / Num. of mol.: 253 / Source method: isolated from a natural source / Formula: H2O
Sequence details
TRUNCATED CONSTRUCT WITH 57 AMINOACIDS IN N TERMINUS AND 10 AMINOACIDS IN C TERMINUS REMOVED.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.03 Å3/Da / Density % sol: 59.35 % / Description: NONE
Crystal grow
Details: PROTEIN: 2UL TCG6PD 10MG/ML, 5MM G6P, 2 MM NADPH; BUFFER: 20 MM TRIS PH 8.0 WITH 0.2 M NACL AND 5 MM ME PRECIPITANT: 1 UL JEFFAMINE ED-2003 PH 7.0 30%, 0.1 M HEPES PH 7.0;
Resolution: 2.65→141.57 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.946 / SU B: 20.601 / SU ML: 0.206 / Cross valid method: THROUGHOUT / ESU R: 0.694 / ESU R Free: 0.288 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22553
2941
5 %
RANDOM
Rwork
0.19991
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obs
0.20118
55569
94.73 %
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Solvent computation
Ion probe radii: 0.9 Å / Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: MASK