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Open data
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Basic information
Entry | Database: PDB / ID: 5a97 | ||||||
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Title | Hazara virus nucleocapsid protain | ||||||
![]() | NUCLEOCAPSID PROTEIN | ||||||
![]() | VIRAL PROTEIN / HAZARA / NUCLEOCAPSID | ||||||
Function / homology | ![]() helical viral capsid / viral nucleocapsid / Hydrolases; Acting on ester bonds / hydrolase activity / ribonucleoprotein complex / RNA binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Surtees, R. / Ariza, A. / Hewson, R. / Barr, J.N. / Edwards, T.A. | ||||||
![]() | ![]() Title: The Crystal Structure of the Hazara Virus Nucleocapsid Protein. Authors: Surtees, R. / Ariza, A. / Punch, E.K. / Trinh, C.H. / Dowall, S.D. / Hewson, R. / Hiscox, J.A. / Barr, J.N. / Edwards, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 527.7 KB | Display | ![]() |
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PDB format | ![]() | 443.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 460.4 KB | Display | ![]() |
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Full document | ![]() | 481.8 KB | Display | |
Data in XML | ![]() | 61.3 KB | Display | |
Data in CIF | ![]() | 84 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4aklS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 54642.137 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 53 % / Description: NONE |
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Crystal grow | pH: 5 Details: 15% PEG 10,000, 0.1 M SODIUM CITRATE PH 5, 0.1 M NACL, 2 % DIOXANE |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS / Detector: PIXEL / Date: Jun 23, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97779 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→65.25 Å / Num. obs: 74079 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 10.5 % / Rmerge(I) obs: 0.11 / Net I/σ(I): 15.1 |
Reflection shell | Resolution: 2.7→2.77 Å / Redundancy: 9 % / Rmerge(I) obs: 0.63 / Mean I/σ(I) obs: 1.7 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4AKL Resolution: 2.7→224.64 Å / Cor.coef. Fo:Fc: 0.93 / Cor.coef. Fo:Fc free: 0.898 / SU B: 22.904 / SU ML: 0.368 / Cross valid method: THROUGHOUT / ESU R Free: 0.397 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 85.501 Å2
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Refinement step | Cycle: LAST / Resolution: 2.7→224.64 Å
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Refine LS restraints |
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