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Open data
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Basic information
Entry | Database: PDB / ID: 4z64 | |||||||||
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Title | the plant peptide hormone receptor complex in arabidopsis | |||||||||
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![]() | HORMONE / hormone receptor complex | |||||||||
Function / homology | ![]() microsporogenesis / regulation of defense response / floral organ abscission / pollen maturation / peptide receptor activity / brassinosteroid mediated signaling pathway / guanylate cyclase / guanylate cyclase activity / embryo development ending in seed dormancy / transmembrane receptor protein serine/threonine kinase activity ...microsporogenesis / regulation of defense response / floral organ abscission / pollen maturation / peptide receptor activity / brassinosteroid mediated signaling pathway / guanylate cyclase / guanylate cyclase activity / embryo development ending in seed dormancy / transmembrane receptor protein serine/threonine kinase activity / receptor serine/threonine kinase binding / Golgi organization / transmembrane receptor protein tyrosine kinase activity / receptor protein-tyrosine kinase / protein autophosphorylation / non-specific serine/threonine protein kinase / protein kinase activity / protein phosphorylation / innate immune response / protein serine kinase activity / signaling receptor binding / protein serine/threonine kinase activity / endoplasmic reticulum membrane / protein-containing complex / mitochondrion / ATP binding / identical protein binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Chai, J. / Wang, J. / Han, Z. | |||||||||
![]() | ![]() Title: Allosteric receptor activation by the plant peptide hormone phytosulfokine Authors: Wang, J. / Li, H. / Han, Z. / Zhang, H. / Wang, T. / Lin, G. / Chang, J. / Yang, W. / Chai, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 174.9 KB | Display | ![]() |
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PDB format | ![]() | 135.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 770.8 KB | Display | ![]() |
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Full document | ![]() | 787.6 KB | Display | |
Data in XML | ![]() | 20.2 KB | Display | |
Data in CIF | ![]() | 29.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4z5wSC ![]() 4z61C ![]() 4z62C ![]() 4z63C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 2 types, 2 molecules AC
#1: Protein | Mass: 69884.008 Da / Num. of mol.: 1 / Fragment: UNP resides 24-648 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: Insect cell expression vector pTIE1 (others) References: UniProt: Q9ZVR7, non-specific serine/threonine protein kinase |
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#2: Protein | Mass: 23981.150 Da / Num. of mol.: 1 / Fragment: UNP residues 1-213 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q94AG2, receptor protein-tyrosine kinase, non-specific serine/threonine protein kinase |
-Protein/peptide / Non-polymers , 2 types, 5 molecules P![](data/chem/img/SO4.gif)
![](data/chem/img/SO4.gif)
#3: Protein/peptide | Mass: 846.879 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
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#6: Chemical | ChemComp-SO4 / |
-Sugars , 2 types, 10 molecules ![](data/chem/img/NAG.gif)
#4: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
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#5: Sugar | ChemComp-NAG / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5 Å3/Da / Density % sol: 75.4 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / Details: 0.1 M sodium acetate pH4.5, 2.0 M (NH4)2SO4 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: BRUKER SMART 6500 / Detector: CCD / Date: Sep 9, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.65→99 Å / Num. obs: 49253 / % possible obs: 96.8 % / Redundancy: 4.2 % / Net I/σ(I): 15.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4Z5W Resolution: 2.659→48.913 Å / SU ML: 0.31 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 29.16 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.659→48.913 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -53.4774 Å / Origin y: -33.4835 Å / Origin z: -8.2839 Å
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Refinement TLS group | Selection details: all |