[English] 日本語
![](img/lk-miru.gif)
- PDB-4w6s: Crystal Structure of Full-Length Split GFP Mutant K126C Disulfide... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 4w6s | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structure of Full-Length Split GFP Mutant K126C Disulfide Dimer, P 43 21 2 Space Group | ||||||
![]() | fluorescent protein E124H/K126C | ||||||
![]() | FLUORESCENT PROTEIN / dimer / disulfide | ||||||
Function / homology | Green Fluorescent Protein / Green fluorescent protein / Beta Barrel / Mainly Beta / PHOSPHATE ION![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Leibly, D.J. / Waldo, G.S. / Yeates, T.O. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: A Suite of Engineered GFP Molecules for Oligomeric Scaffolding. Authors: Leibly, D.J. / Arbing, M.A. / Pashkov, I. / DeVore, N. / Waldo, G.S. / Terwilliger, T.C. / Yeates, T.O. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 191.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 154.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 4w69C ![]() 4w6aC ![]() 4w6bC ![]() 4w6cC ![]() 4w6dC ![]() 4w6fC ![]() 4w6gC ![]() 4w6hC ![]() 4w6iC ![]() 4w6jC ![]() 4w6kC ![]() 4w6lC ![]() 4w6mC ![]() 4w6nC ![]() 4w6oC ![]() 4w6pC ![]() 4w6rC ![]() 4w6tC ![]() 4w6uC ![]() 4w72C ![]() 4w73C ![]() 4w74C ![]() 4w75C ![]() 4w76C ![]() 4w77C ![]() 4w7aC ![]() 4w7cC ![]() 4w7dC ![]() 4w7eC ![]() 4w7fC ![]() 4w7rC ![]() 4w7xC ![]() 2b3pS C: citing same article ( S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 26039.307 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-GOL / |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 4.12 Å3/Da / Density % sol: 70.16 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.2 / Details: 40% PEG300, 0.1M Phosphate-citrate pH 4.2 |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Dec 8, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9789 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→68.28 Å / Num. obs: 16549 / % possible obs: 100 % / Redundancy: 25.3 % / Rsym value: 0.257 / Net I/σ(I): 18.03 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 2B3P Resolution: 3.1→68.28 Å / SU ML: 0.43 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 28.6 / Stereochemistry target values: ML
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→68.28 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group |
|