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- PDB-4w72: Crystal Structure of Full-Length Split GFP Mutant E115C/T118H Dis... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4w72 | ||||||
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Title | Crystal Structure of Full-Length Split GFP Mutant E115C/T118H Disulfide Dimer With Copper Mediated Crystal Contacts, P 21 21 21, Form 1 | ||||||
![]() | fluorescent protein E115C/T118H | ||||||
![]() | FLUORESCENT PROTEIN / dimer / disulfide | ||||||
Function / homology | Green Fluorescent Protein / Green fluorescent protein / Beta Barrel / Mainly Beta / COPPER (II) ION![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Leibly, D.J. / Waldo, G.S. / Yeates, T.O. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A Suite of Engineered GFP Molecules for Oligomeric Scaffolding. Authors: Leibly, D.J. / Arbing, M.A. / Pashkov, I. / DeVore, N. / Waldo, G.S. / Terwilliger, T.C. / Yeates, T.O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 199.5 KB | Display | ![]() |
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PDB format | ![]() | 159.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4w69C ![]() 4w6aC ![]() 4w6bC ![]() 4w6cC ![]() 4w6dC ![]() 4w6fC ![]() 4w6gC ![]() 4w6hC ![]() 4w6iC ![]() 4w6jC ![]() 4w6kC ![]() 4w6lC ![]() 4w6mC ![]() 4w6nC ![]() 4w6oC ![]() 4w6pC ![]() 4w6rC ![]() 4w6sC ![]() 4w6tC ![]() 4w6uC ![]() 4w73C ![]() 4w74C ![]() 4w75C ![]() 4w76C ![]() 4w77C ![]() 4w7aC ![]() 4w7cC ![]() 4w7dC ![]() 4w7eC ![]() 4w7fC ![]() 4w7rC ![]() 4w7xC ![]() 2b3pS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26067.383 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() #2: Chemical | ChemComp-CU / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.69 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.5 / Details: 20% PEG3000, 0.1M Acetate pH 4.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Dec 8, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.996→57.12 Å / Num. obs: 33399 / % possible obs: 99.7 % / Redundancy: 7.1 % / Rsym value: 0.05998 / Net I/σ(I): 16.59 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 2B3P Resolution: 2→57.12 Å / SU ML: 0.19 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 25.58 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→57.12 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -18.6337 Å / Origin y: -15.4416 Å / Origin z: 10.8069 Å
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Refinement TLS group | Selection details: ALL |