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- PDB-4v4i: Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Function... -

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Basic information

Entry
Database: PDB / ID: 4v4i
TitleCrystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements.
Components
  • (30S ribosomal protein ...) x 20
  • (50S ribosomal protein ...) x 28
  • 16S SMALL SUBUNIT RIBOSOMAL RNA
  • 23S LARGE SUBUNIT RIBOSOMAL RNA
  • 5S LARGE SUBUNIT RIBOSOMAL RNA
  • E-TRNA
  • MRNA
  • P-site PHE-tRNA
KeywordsRIBOSOME / FUNCTIONAL COMPLEX / TRNA / E site / P site / PEPTIDYL TRANSFERASE CENTER
Function / homology
Function and homology information


large ribosomal subunit / regulation of translation / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit ...large ribosomal subunit / regulation of translation / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / tRNA binding / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / zinc ion binding / metal ion binding / cytoplasm / cytosol
Similarity search - Function
Ribosomal protein L1, bacterial-type / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site ...Ribosomal protein L1, bacterial-type / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S19, bacterial-type / Ribosomal protein L20 signature. / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein S13, bacterial-type / Ribosomal protein L27 signature. / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / : / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L16 / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein L20 / Ribosomal protein S16 domain superfamily / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein S15, bacterial-type / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein S6
Similarity search - Domain/homology
: / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bTHX ...: / : / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bTHX / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein uL1 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35
Similarity search - Component
Biological speciesThermus thermophilus (bacteria)
Escherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.71 Å
AuthorsKorostelev, A. / Trakhanov, S. / Laurberg, M. / Noller, H.F.
CitationJournal: Cell(Cambridge,Mass.) / Year: 2006
Title: Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements
Authors: Korostelev, A. / Trakhanov, S. / Laurberg, M. / Noller, H.F.
History
DepositionFeb 15, 2007Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 9, 2014Provider: repository / Type: Initial release
SupersessionDec 10, 2014ID: 1VSA, 2OW8
Revision 1.1Dec 10, 2014Group: Other
Revision 2.0Jul 3, 2019Group: Data collection / Derived calculations ...Data collection / Derived calculations / Non-polymer description / Structure summary
Category: chem_comp / entity / struct_conn
Item: _chem_comp.formula / _chem_comp.formula_weight ..._chem_comp.formula / _chem_comp.formula_weight / _entity.formula_weight / _struct_conn.pdbx_leaving_atom_flag
Revision 2.1Apr 3, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq_dif.details
Remark 400COMPOUND THE COMPLETE ASYMMETRIC UNIT CONTAINS 30S AND 50S RIBOSOMAL SUBUNITS. THIS ENTRY 1VS9 ...COMPOUND THE COMPLETE ASYMMETRIC UNIT CONTAINS 30S AND 50S RIBOSOMAL SUBUNITS. THIS ENTRY 1VS9 CONTAINS 50S RIBOSOMAL SUBUNIT. THE 30S RIBOSOMAL SUBUNIT CAN BE FOUND IN PDB ENTRY 2I1C.
Remark 999SEQUENCE The sequence for 23S LARGE SUBUNIT RIBOSOMAL RNA corresponds to the sequence from GB entry ...SEQUENCE The sequence for 23S LARGE SUBUNIT RIBOSOMAL RNA corresponds to the sequence from GB entry AE017221, residues 1534489-1537381. Residue (w C 41 ) and Residue (w G 43 ) are not linked Distance of O3*-P bond is 1.86 Residue (w U 99 ) and Residue (w G 101 ) are not linked Distance of O3*-P bond is 1.85 Residue (w C 155 ) and Residue (w U 161 ) are not linked Distance of O3*-P bond is 1.82 Residue (w U 165 ) and Residue (w G 171 ) are not linked Distance of O3*-P bond is 1.79 Residue (w C 366 B) and Residue (w G 370 ) are not linked Distance of O3*-P bond is 1.80 Residue (w C 436 ) and Residue (w G 438 ) are not linked Distance of O3*-P bond is 1.77 Residue (w G 489 ) and Residue (w G 491 ) are not linked Distance of O3*-P bond is 1.82 Residue (w C 537 ) and Residue (w G 539 ) are not linked Distance of O3*-P bond is 1.76 Residue (w A 890 ) and Residue (w G 892 ) are not linked Distance of O3*-P bond is 1.94 Residue (w A 926 ) and Residue (w G 928 ) are not linked Distance of O3*-P bond is 1.97 Residue (w U 1133 ) and Residue (w C 1135 ) are not linked Distance of O3*-P bond is 1.86 Residue (w A 114 B) and Residue (w A 1143 ) are not linked Distance of O3*-P bond is 1.81 Residue (w G 1171 ) and Residue (w G 1173 ) are not linked Distance of O3*-P bond is 1.94 Residue (w C 122 A) and Residue (w C 1222 ) are not linked Distance of O3*-P bond is 1.80 Residue (w A 144 B) and Residue (w C 1445 ) are not linked Distance of O3*-P bond is 1.77 Residue (w A 149 B) and Residue (w G 1449 ) are not linked Distance of O3*-P bond is 1.85 Residue (w C 1451 ) and Residue (w A 1453 ) are not linked Distance of O3*-P bond is 1.78 Residue (w U 1481 ) and Residue (w G 1483 ) are not linked Distance of O3*-P bond is 1.90 Residue (w C 1506 ) and Residue (w A 1508 ) are not linked Distance of O3*-P bond is 1.96 Residue (w C 154 B) and Residue (w C 1547 ) are not linked Distance of O3*-P bond is 1.90 Residue (w A 1583 ) and Residue (w C 1585 ) are not linked Distance of O3*-P bond is 1.77 Residue (w C 163 B) and Residue (w A 1631 ) are not linked Distance of O3*-P bond is 1.79 Residue (w C 1712 ) and Residue (w U 1716 ) are not linked Distance of O3*-P bond is 1.86 Residue (w U 1735 ) and Residue (w C 1741 ) are not linked Distance of O3*-P bond is 1.82 Residue (w G 1743 ) and Residue (w G 1746 ) are not linked Distance of O3*-P bond is 1.89 Residue (w U 1864 ) and Residue (w G 1869 ) are not linked Distance of O3*-P bond is 1.83 Residue (w A 1872 ) and Residue (w G 1878 ) are not linked Distance of O3*-P bond is 1.79 Residue (w A 2199 ) and Residue (w C 2205 ) are not linked Distance of O3*-P bond is 1.82 Residue (w U 2213 ) and Residue (w G 2215 ) are not linked Distance of O3*-P bond is 1.81 Residue (w G 2219 ) and Residue (w G 2224 ) are not linked Distance of O3*-P bond is 1.83 Residue (w A 712 B) and Residue (w A 2713 ) are not linked Distance of O3*-P bond is 1.75
Remark 300BIOMOLECULE: 1 THIS ENTRY CONTAINS PART OF THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT. THE BIOLOGICAL ...BIOMOLECULE: 1 THIS ENTRY CONTAINS PART OF THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT. THE BIOLOGICAL UNIT CONSISTS OF TWO SUBUNITS. THERE IS 1 BIOLOGICAL UNIT IN THE ASYMMETRIC UNIT.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
w: 23S LARGE SUBUNIT RIBOSOMAL RNA
x: 5S LARGE SUBUNIT RIBOSOMAL RNA
A: 50S ribosomal protein L1
B: 50S ribosomal protein L2
C: 50S ribosomal protein L3
D: 50S ribosomal protein L4
E: 50S ribosomal protein L5
F: 50S ribosomal protein L6
G: 50S ribosomal protein L9
H: 50S ribosomal protein L13
I: 50S ribosomal protein L14
J: 50S ribosomal protein L15
K: 50S ribosomal protein L16
L: 50S ribosomal protein L17
M: 50S ribosomal protein L18
N: 50S ribosomal protein L19
O: 50S ribosomal protein L20
P: 50S ribosomal protein L21
Q: 50S ribosomal protein L22
R: 50S ribosomal protein L23
S: 50S ribosomal protein L24
T: 50S ribosomal protein L25
U: 50S ribosomal protein L27
V: 50S ribosomal protein L28
W: 50S ribosomal protein L29
X: 50S ribosomal protein L30
Y: 50S ribosomal protein L32
Z: 50S ribosomal protein L34
a: 50S ribosomal protein L35
b: 50S ribosomal protein L36
y: 16S SMALL SUBUNIT RIBOSOMAL RNA
z: P-site PHE-tRNA
0: E-TRNA
1: MRNA
c: 30S ribosomal protein S2
d: 30S ribosomal protein S3
e: 30S ribosomal protein S4
f: 30S ribosomal protein S5
g: 30S ribosomal protein S6
h: 30S ribosomal protein S7
i: 30S ribosomal protein S8
j: 30S ribosomal protein S9
k: 30S ribosomal protein S10
l: 30S ribosomal protein S11
m: 30S ribosomal protein S12
n: 30S ribosomal protein S13
o: 30S ribosomal protein S14
p: 30S ribosomal protein S15
q: 30S ribosomal protein S16
r: 30S ribosomal protein S17
s: 30S ribosomal protein S18
t: 30S ribosomal protein S19
u: 30S ribosomal protein S20
v: 30S ribosomal protein Thx


Theoretical massNumber of molelcules
Total (without water)2,224,69954
Polymers2,224,69954
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)507.810, 507.810, 689.520
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number97
Space group name H-MI422

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Components

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RNA chain , 6 types, 6 molecules wxyz01

#1: RNA chain 23S LARGE SUBUNIT RIBOSOMAL RNA


Mass: 939562.188 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27
#2: RNA chain 5S LARGE SUBUNIT RIBOSOMAL RNA


Mass: 39211.414 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: GenBank: 48271
#31: RNA chain 16S SMALL SUBUNIT RIBOSOMAL RNA


Mass: 494210.781 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: GenBank: 155076
#32: RNA chain P-site PHE-tRNA


Mass: 24649.912 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: GenBank: 174422
#33: RNA chain E-TRNA


Mass: 24502.570 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27
#34: RNA chain MRNA


Mass: 3146.917 Da / Num. of mol.: 1 / Source method: obtained synthetically
Details: 10-NUCLEOTIDE SYNTHETIC MRNA SEQUENCE 'AUGUUCUAAA' (DHARMACON RNA TECHNOLOGIES)

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50S ribosomal protein ... , 28 types, 28 molecules ABCDEFGHIJKLMNOPQRSTUVWXYZab

#3: Protein 50S ribosomal protein L1


Mass: 24867.699 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72GV9
#4: Protein 50S ribosomal protein L2


Mass: 30532.551 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I07
#5: Protein 50S ribosomal protein L3


Mass: 22450.213 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I04
#6: Protein 50S ribosomal protein L4


Mass: 22744.279 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I05
#7: Protein 50S ribosomal protein L5


Mass: 21075.623 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I16
#8: Protein 50S ribosomal protein L6


Mass: 19568.926 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I19
#9: Protein 50S ribosomal protein L9


Mass: 16436.205 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72GV5
#10: Protein 50S ribosomal protein L13


Mass: 18534.008 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72IN1
#11: Protein 50S ribosomal protein L14


Mass: 13309.586 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I14
#12: Protein 50S ribosomal protein L15


Mass: 16319.142 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I23
#13: Protein 50S ribosomal protein L16


Mass: 15977.909 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I11
#14: Protein 50S ribosomal protein L17


Mass: 13750.148 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I33
#15: Protein 50S ribosomal protein L18


Mass: 12639.845 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I20
#16: Protein 50S ribosomal protein L19


Mass: 17217.867 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72JU9
#17: Protein 50S ribosomal protein L20


Mass: 13779.275 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72L76
#18: Protein 50S ribosomal protein L21


Mass: 11069.153 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72HR2
#19: Protein 50S ribosomal protein L22


Mass: 12808.055 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I09
#20: Protein 50S ribosomal protein L23


Mass: 10759.808 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I06
#21: Protein 50S ribosomal protein L24


Mass: 12085.611 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I15
#22: Protein 50S ribosomal protein L25


Mass: 23238.572 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72IA7
#23: Protein 50S ribosomal protein L27


Mass: 9501.061 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72HR3
#24: Protein 50S ribosomal protein L28


Mass: 11032.174 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72G84
#25: Protein 50S ribosomal protein L29


Mass: 8670.258 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I12
#26: Protein 50S ribosomal protein L30


Mass: 6799.126 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I22
#27: Protein 50S ribosomal protein L32


Mass: 6734.104 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62652
#28: Protein/peptide 50S ribosomal protein L34


Mass: 6132.449 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P80340
#29: Protein 50S ribosomal protein L35


Mass: 7506.178 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72L77
#30: Protein/peptide 50S ribosomal protein L36


Mass: 4435.411 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I28

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30S ribosomal protein ... , 20 types, 20 molecules cdefghijklmnopqrstuv

#35: Protein 30S ribosomal protein S2


Mass: 29317.703 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62662
#36: Protein 30S ribosomal protein S3


Mass: 26751.076 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62663
#37: Protein 30S ribosomal protein S4


Mass: 24373.447 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62664
#38: Protein 30S ribosomal protein S5


Mass: 17583.416 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62665
#39: Protein 30S ribosomal protein S6


Mass: 11988.753 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62666
#40: Protein 30S ribosomal protein S7


Mass: 18050.973 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62667
#41: Protein 30S ribosomal protein S8


Mass: 15868.570 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62668
#42: Protein 30S ribosomal protein S9


Mass: 14429.661 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62669
#43: Protein 30S ribosomal protein S10


Mass: 11954.968 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62653
#44: Protein 30S ribosomal protein S11


Mass: 13737.868 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62654
#45: Protein 30S ribosomal protein S12


Mass: 14637.384 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P61941
#46: Protein 30S ribosomal protein S13


Mass: 14338.861 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62655
#47: Protein 30S ribosomal protein S14


Mass: 7158.725 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62656
#48: Protein 30S ribosomal protein S15


Mass: 10578.407 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62657
#49: Protein 30S ribosomal protein S16


Mass: 10409.983 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62238
#50: Protein 30S ribosomal protein S17


Mass: 12324.670 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62658
#51: Protein 30S ribosomal protein S18


Mass: 10258.299 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62659
#52: Protein 30S ribosomal protein S19


Mass: 10605.464 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62660
#53: Protein 30S ribosomal protein S20


Mass: 11722.116 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62661
#54: Protein/peptide 30S ribosomal protein Thx


Mass: 3350.030 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62613

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 5 Å3/Da / Density % sol: 75.38 %
Crystal growTemperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: 24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Components of the solutions
IDNameCrystal-IDSol-ID
1MPD11
2H2O11
3MPD12
4H2O12

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 12.3.1
DetectorType: ADSC QUANTUM 315 / Detector: CCD
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthRelative weight: 1
ReflectionResolution: 3.7→75 Å / Num. obs: 465894 / % possible obs: 99.3 % / Observed criterion σ(I): 1.5 / Redundancy: 5.4 % / Rmerge(I) obs: 0.149
Reflection shellResolution: 3.7→3.8 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.467 / % possible all: 96.6

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Processing

Software
NameVersionClassificationNB
REFMAC5.2.0019refinement
PDB_EXTRACT2data extraction
ADSCQUANTUMdata collection
CNSrefinement
d*TREKdata reduction
CCP4(SCALA)data scaling
CNSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: pdb entries 1TWT AND 1TWV

1twt

1twv


Resolution: 3.71→30 Å / Cor.coef. Fo:Fc: 0.835 / Cor.coef. Fo:Fc free: 0.856 / SU B: 200.673 / SU ML: 1.295 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.759 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: This file represents the structure 1VS9 re-refined with strong secondary-structure restraints. Structures of proteins L15, L19, L21, L28 and L29 from PDB entry 2J03 (Selmer et al., 2006) ...Details: This file represents the structure 1VS9 re-refined with strong secondary-structure restraints. Structures of proteins L15, L19, L21, L28 and L29 from PDB entry 2J03 (Selmer et al., 2006) were used as starting models in refinement.
RfactorNum. reflection% reflectionSelection details
Rfree0.353 11345 2.5 %RANDOM
Rwork0.348 ---
all0.349 ---
obs0.349 455685 100 %-
Solvent computationIon probe radii: 1.94 Å / Shrinkage radii: 1.1 Å / VDW probe radii: 1.92 Å / Solvent model: MASK
Refinement stepCycle: LAST / Resolution: 3.71→30 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms26862 64786 0 0 91648
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0110.021159290
X-RAY DIFFRACTIONr_angle_refined_deg1.3932.731238258
X-RAY DIFFRACTIONr_dihedral_angle_1_deg2.79555738
X-RAY DIFFRACTIONr_dihedral_angle_2_deg41.00921.2182061
X-RAY DIFFRACTIONr_dihedral_angle_3_deg17.715159016
X-RAY DIFFRACTIONr_dihedral_angle_4_deg16.42115666
X-RAY DIFFRACTIONr_chiral_restr0.1090.230224
X-RAY DIFFRACTIONr_gen_planes_refined0.0150.0283941
X-RAY DIFFRACTIONr_nbd_refined0.4170.289280
X-RAY DIFFRACTIONr_nbtor_refined0.3220.266600
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.3260.25400
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.4880.268
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.3660.210
X-RAY DIFFRACTIONr_mcbond_it25.3571.529656
X-RAY DIFFRACTIONr_mcangle_it35.427246261
X-RAY DIFFRACTIONr_scbond_it5.2083181307
X-RAY DIFFRACTIONr_scangle_it10.1254.5191997
LS refinement shellResolution: 3.71→3.805 Å / Total num. of bins used: 20
Num. reflection% reflection
Rfree630 -
Rwork31881 -
obs32511 100 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.3544-0.6603-0.21461.23040.39990.130.22740.08440.21610.1777-0.3107-0.04340.0876-0.06910.0833000-0.0001-0.00010.0001-103.26791.8061-143.0996
20.66240.5669-0.05320.4851-0.04550.00430.0032-0.26330.1722-0.1843-0.0463-0.0243-0.0173-0.00950.0431000000-111.303789.6303-146.2364
30.0226-0.10690.00910.5053-0.04310.00370.0578-0.01550.05070.0759-0.122-0.0824-0.19160.34060.0642000000-122.766441.6755-59.6841
4000000-0.0299-0.0342-0.1511-0.01810.0406-0.0067-0.0334-0.1056-0.0107000000-113.313150.5275-69.8557
50.73310.2112-0.05930.3816-0.07610.0157-0.05970.0002-0.0753-0.05240.13010.0417-0.1084-0.0817-0.07040000-0.00010-203.6106-15.3518-60.7766
60.11310.048-0.0280.0204-0.01190.0069-0.0493-0.1043-0.06550.01080.09730.0551-0.0027-0.043-0.048000000-205.446992.1684-95.5842
70.00110.01270.00220.15260.02650.00460.06390.0040.153-0.0253-0.03720.061-0.02340.0349-0.0267000000-138.59519.9307-185.9234
80.03830.1129-0.01510.3329-0.04450.00590.0370.1292-0.18490.0472-0.04170.0282-0.02670.07620.0047000000-211.3408-43.7213-109.4818
90.2821-0.08850.00280.0278-0.000900.13220.2023-0.03580.1059-0.0086-0.06920.03840.024-0.1236000.0001-0.000100-92.671122.487-83.8152
100.57810.1688-0.14630.0493-0.04270.037-0.00650.0745-0.1014-0.0131-0.00850.0358-0.10430.06020.015000000-227.5694-0.3169-95.53
11000000-0.02490.07940.1396-0.03230.0120.1399-0.1781-0.09130.013000000-160.8441-27.6102-67.4687
120.46330.3298-0.20030.2347-0.14250.08660.0197-0.15670.04830.0632-0.01050.02920.02-0.0347-0.0093000000-182.853485.9409-124.4711
130.1537-0.11370.04350.0841-0.03220.01230.1167-0.0416-0.00380.0419-0.0991-0.11550.0049-0.0169-0.0176000000-176.8074-1.3562-138.4532
140000000.0293-0.0379-0.054-0.1153-0.0163-0.17720.08510.0394-0.0129000000-192.43688.9255-26.1245
150.09570.08480.01920.07520.0170.00380.0278-0.0363-0.05770.0641-0.1786-0.00150.0677-0.11770.1508000000-170.158751.1147-187.1672
160.6399-0.72270.38780.8162-0.43790.2350.0499-0.1988-0.11060.29490.0212-0.0624-0.1921-0.221-0.071100.00010-0.000100-173.1533-30.0552-38.989
170.1649-0.07080.0010.0304-0.00040-0.0972-0.12360.0296-0.08790.04850.0274-0.1969-0.08140.0487000000-228.07530.783-102.2491
180.1837-0.2695-0.03260.39530.04780.00580.0595-0.1151-0.03380.1679-0.12620.11910.13930.03970.0667-0.000100.00010-0.00010-240.859841.1482-108.1994
191.0589-0.4140.22470.1619-0.08790.0477-0.1545-0.18390.0171-0.01360.1294-0.0669-0.09280.1290.0252000000-212.073638.633-50.3349
202.03760.18720.18070.3422-0.01460.0190.01710.1039-0.0453-0.08880.15910.01660.0531-0.013-0.1762000000-171.145773.7041-24.3737
210.06630.4353-0.15642.8592-1.02710.3689-0.00160.0047-0.0760.3108-0.22850.1661-0.25770.26880.2301-0.00010.00010.0001000.0001-214.449694.7914-47.159
220.03880.06430.01310.10630.02170.00440.0048-0.0573-0.03240.07330.0674-0.00180.0255-0.0265-0.0722000000-191.1791-6.3166-163.9043
230.26330.33220.20340.4190.25660.1571-0.0555-0.21990.121-0.27580.1132-0.06540.26310.0277-0.057700-0.0001000-181.254447.3272-155.0396
240.05420.0024-0.00780.0001-0.00040.0011-0.20130.23660.1902-0.15030.15790.0932-0.14310.35570.0434-0.00010.0001-0.0001-0.000100-134.336484.4061-86.9231
251.1056-0.61670.02550.344-0.01420.00060.0302-0.04690.2412-0.06750.16220.06050.1502-0.1199-0.1924000000-177.540696.1864-29.4197
26000000-0.02070.10560.0936-0.07960.0378-0.10030.16620.1377-0.0171000000-216.785735.3309-137.5719
270.435-0.1679-0.86470.07180.40542.4523-0.0119-0.1561-0.067-0.07640.0615-0.1467-0.0147-0.0286-0.0496000000-211.135524.2089-45.053
281.71951.60060.42861.51420.43110.14950.0121-0.0443-0.01260.0758-0.1193-0.05920.00210.02280.1072000000-163.841763.5747-60.1538
290.27720.08580.10850.02650.03360.04240.0320.2095-0.0453-0.1372-0.10.15230.1274-0.0230.068000000.0001-170.842968.8386-129.851
302.0767-1.2002-0.26090.69360.15080.0328-0.00240.0198-0.0341-0.0303-0.0331-0.06840.04250.16830.03550-0.00010000-192.8783-25.5438-119.5695
31000000-0.0680.02510.00190.01070.04430.1023-0.04070.02390.0237000000-36.6983-18.2404-98.8584
32000000-0.0570.07390.00930.0108-0.0311-0.1307-0.1966-0.08150.0881000000-69.4548-46.7528-140.8621
330.1073-0.03860.00530.0139-0.00190.0003-0.001-0.0557-0.0189-0.0401-0.0561-0.01530.10370.03360.0572000000-82.8222-73.1081-86.3789
340.0174-0.0636-0.02420.23290.08880.03380.0371-0.007-0.0050.1001-0.01850.1103-0.04420.0599-0.0186000000-68.2852-39.5826-89.2215
350.26970.4491-0.37970.7479-0.63230.53460.0160.0222-0.14320.0164-0.01020.19940.11230.1998-0.00580-0.00010000-71.754149.6531-69.769
360.23970.17030.04820.12090.03420.0097-0.0203-0.0959-0.1464-0.00410.08980.08930.16530.1487-0.0694000000-77.270623.0509-151.5108
370.5756-0.1633-0.17920.04630.05090.0558-0.0394-0.0203-0.0331-0.08020.0714-0.0179-0.02310.0086-0.032000000-61.2994-25.5639-60.7521
380.139-0.14010.01980.1412-0.020.00280.0538-0.15680.17050.04420.0679-0.003-0.1391-0.2071-0.1216000000-60.3862.1384-169.6574
39000000-0.03050.1772-0.1702-0.092-0.20350.2029-0.1762-0.11720.234-0.00010000-0.0001-60.5287-35.3715-167.3943
40000000-0.0246-0.06920.0580.268-0.0373-0.08780.0978-0.28410.0619-0.000100000-79.671342.5065-113.1098
410.4933-0.28020.1950.1592-0.11080.07710.0312-0.0278-0.0641-0.1454-0.06050.0476-0.12490.18370.029300.0001-0.0001000-116.4404-39.0827-91.5152
420.08360.2273-0.24050.6183-0.65410.6918-0.0212-0.0558-0.15410.1273-0.1029-0.0057-0.01540.13430.1241000000-114.6906-0.2901-176.7415
431.78540.32671.34710.05990.24651.01640.00290.4592-0.1202-0.12250.1225-0.0735-0.11610.2352-0.125400-0.0001-0.00020.00010-86.1233-38.5105-156.6972
440.00070.0273-0.00171.0859-0.06930.00440.15910.042-0.09970.1657-0.0335-0.0878-0.0161-0.0269-0.125600-0.0001000-81.14815.1051-45.9683
450.83690.39920.25480.19040.12150.0776-0.1930.1101-0.2679-0.01890.18290.0879-0.44190.32560.010200.00020-0.00010-0.0001-102.4784-69.5385-40.3601
460.19990.02540.25810.0040.02390.44020.09040.0069-0.0628-0.0820.17580.1923-0.1294-0.0791-0.266200-0.0001000-97.9849-15.4751-37.3998
470.6089-0.70220.30320.8099-0.34970.1510.00220.4515-0.00670.02650.0810.18510.2666-0.1398-0.0833-0.000100.0001-0.00010.00010-61.895335.4598-87.3048
480.4275-0.1889-0.16540.08350.07310.064-0.03750.00550.1440.00770.0293-0.12310.22810.15040.00820-0.00010000-123.8417-33.0779-173.76
490.01280.0611-0.00640.2918-0.03060.0032-0.09940.09-0.0434-0.09760.0920.03210.05320.210.007400.00010000-132.0952-38.0054-15.0598
503.0864-2.78980.53252.5218-0.48140.09190.00790.3763-0.1137-0.12530.0218-0.03810.07830.124-0.0297-0.000100-0.00010.00010-93.7656-8.426-177.7247
510.1218-0.03140.06460.1881-0.06160.04550.03330.07020.005-0.05910.02590.02640.0165-0.0396-0.0592000000-178.973426.269-177.4783
520.0707-0.0261-0.0190.0268-0.01560.0350.0024-0.0147-0.01160.00490.0097-0.01210.028-0.0099-0.01210.0010.0035-0.00290.0004-0.00690.001-117.1086-55.896-57.2147
530.0291-0.0333-0.01750.0784-0.08330.27560.02240.01990.05680.0050.02630.00880.0159-0.0818-0.0487000000-121.0991-14.0268-74.3052
540.1779-0.09230.01020.1432-0.04360.0160.0140.01180.0274-0.0231-0.01330.0048-0.00460.0044-0.00070000-0.00010.0001-84.2258-21.4099-153.9137
550.1404-0.0420.01790.1662-0.06850.02830.0046-0.0010.01910.0233-0.0093-0.00190.00480.02020.004700000.00010-85.72681.0431-75.0807
561.0816-0.1735-0.68130.33080.24370.4888-0.01220.0075-0.1826-0.0533-0.12160.04-0.10780.06560.1338000000-141.648917.7788-130.351
573.9176-1.0147-1.45513.6640.56970.55140.0165-0.44880.1484-0.19490.12750.0124-0.0119-0.2668-0.1440-0.000100.0001-0.00020-108.41061.2524-133.3145
580.9603-0.0303-0.03360.9657-0.06110.00520.03490.03720.0014-0.11050.05430.0543-0.04480.0669-0.0892000000-117.720151.3728-134.8397
591.52910.8776-0.90860.5036-0.52150.540.01820.49690.10380.3709-0.1325-0.00290.1569-0.10020.1143000.0001-0.000100-97.64221.9063-135.974
601.9414-2.4691.41043.14-1.79371.02470.0252-0.2452-0.1826-0.0210.09870.0795-0.0251-0.1067-0.1239000000-97.77423.047-118.3364
611.38240.0696-0.91051.36580.67573.3946-0.0078-0.08680.26540.057-0.01810.02340.0163-0.1620.0259000000-243.82220.6255-74.5807
620.0929-0.02050.00010.07360.00330.0002-0.0059-0.01520.02640.01710.00360.0112-0.021-0.00720.00230.0068-0.0172-0.00040.00260.01470.0023-175.217574.1092-79.5122
630.8146-0.3524-0.31480.68880.28420.2675-0.0339-0.0305-0.0821-0.09420.0636-0.05070.01140.0593-0.0297000000-175.59216.5672-153.092
640.83210.1997-0.01180.1618-0.03610.00990.0478-0.0256-0.01840.0074-0.03220.0366-0.0539-0.0106-0.0155000000-201.580631.8772-119.179
650.3663-0.073-0.03250.45950.25720.2751-0.02110.0566-0.0485-0.00830.0560.04160.08410.0727-0.0349000000-198.8776-26.5237-129.5166
660.08050.00150.00410.0484-0.0040.00060.0119-0.0096-0.00530.0168-0.00580.0041-0.00280.0027-0.0060.00040.0017-0.0042-0.00020.0010-157.75138.0867-61.1182
670.14480.011-0.07780.17980.09230.0956-0.0221-0.00480.0139-0.064-0.00150.0227-0.04690.04060.0237000000-94.092775.4993-123.8168
680.5354-0.1367-0.05180.0987-0.05960.0882-0.0060.0196-0.1066-0.0215-0.0048-0.1030.00210.0280.0108000000-147.738849.8493-103.892
690.2002-0.09260.03570.11110.06110.09460.0010.0121-0.0051-0.03380.0048-0.001-0.04720.004-0.0058000000-159.433948.3785-149.2929
700.0912-0.01650.0350.0129-0.00980.01460.0031-0.0109-0.01040.005-0.0059-0.00320.0027-0.03040.0028-0.0013-0.00360.01810.00210.02230.0014-187.8327-3.668-74.7106
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION / Selection: ALL

IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11AC6 - 646 - 64
22AC162 - 229162 - 229
33BD2 - 552 - 55
44BD56 - 22156 - 221
54BD222 - 273222 - 273
65CE3 - 1053 - 105
75CE106 - 160106 - 160
85CE161 - 203161 - 203
96DF4 - 1974 - 197
107EG3 - 1823 - 182
118FH7 - 817 - 81
128FH82 - 17982 - 179
139GI1 - 331 - 33
149GI34 - 7934 - 79
159GI80 - 14880 - 148
1610HJ24 - 16124 - 161
1711IK1 - 1221 - 122
1812JL5 - 685 - 68
1912JL69 - 15069 - 150
2013KM1 - 1371 - 137
2114LN1 - 1181 - 118
2215MO7 - 597 - 59
2315MO60 - 11260 - 112
2416NP1 - 1371 - 137
2517OQ2 - 722 - 72
2617OQ73 - 11873 - 118
2718PR1 - 1011 - 101
2819QS2 - 1102 - 110
2920RT3 - 943 - 94
3021SU1 - 1031 - 103
3122TV1 - 941 - 94
3222TV95 - 18595 - 185
3323UW10 - 3310 - 33
3423UW34 - 8534 - 85
3524VX8 - 958 - 95
3625WY1 - 361 - 36
3725WY37 - 6237 - 62
3826XZ1 - 601 - 60
3927YAA5 - 605 - 60
4028ZBA1 - 481 - 48
4129aCA2 - 642 - 64
4230bDA2 - 362 - 36
4331cIA7 - 2407 - 240
4432dJA2 - 2072 - 207
4533eKA2 - 2092 - 209
4634fLA5 - 1545 - 154
4735gMA1 - 1011 - 101
4836hNA2 - 1562 - 156
4937iOA1 - 1381 - 138
5038jPA2 - 1282 - 128
5139kQA3 - 1003 - 100
5240lRA11 - 12611 - 126
5341mSA4 - 1272 - 125
5442nTA2 - 1262 - 126
5543oUA2 - 612 - 61
5644pVA2 - 892 - 89
5745qWA1 - 831 - 83
5846rXA2 - 1052 - 105
5947sYA16 - 8816 - 88
6048tZA2 - 812 - 81
6149uAB8 - 1068 - 106
6250vBB2 - 252 - 25
6351xB1 - 1192 - 121
6452yEA5 - 5606 - 544
6553yEA1391 - 15291374 - 1507
6654yEA927 - 1390905 - 1373
6755yEA561 - 926545 - 904
6856zFA1 - 251 - 25
6956zFA45 - 7645 - 76
7057zFA26 - 4426 - 44
71580GA1 - 251 - 25
72580GA45 - 7645 - 76
73590GA26 - 4426 - 44
74601HA-3 - 31 - 6
7561wA1 - 101 - 10
7661wA2895 - 29022882 - 2889
7762wA11 - 84611 - 872
7863wA847 - 932873 - 956
7964wA933 - 1005957 - 1030
8065wA1006 - 11491031 - 1174
8166wA1150 - 20921175 - 2092
8267wA2093 - 22252093 - 2215
8368wA2226 - 22582216 - 2248
8469wA2259 - 24322249 - 2422
8570wA2433 - 28942423 - 2881

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