+Open data
-Basic information
Entry | Database: PDB / ID: 4j8b | ||||||
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Title | Crystal structure of alpha-COP/Emp47p complex | ||||||
Components |
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Keywords | PROTEIN TRANSPORT / beta propeller domain / Dilysine motif / ER retrieval | ||||||
Function / homology | Function and homology information COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / COPI vesicle coat / intra-Golgi vesicle-mediated transport / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / endoplasmic reticulum to Golgi vesicle-mediated transport / intracellular protein transport / Golgi membrane / structural molecule activity / Golgi apparatus / cytoplasm Similarity search - Function | ||||||
Biological species | Schizosaccharomyces pombe (fission yeast) Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.878 Å | ||||||
Authors | Ma, W. / Goldberg, J. | ||||||
Citation | Journal: Embo J. / Year: 2013 Title: Rules for the recognition of dilysine retrieval motifs by coatomer. Authors: Ma, W. / Goldberg, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4j8b.cif.gz | 76.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4j8b.ent.gz | 57.2 KB | Display | PDB format |
PDBx/mmJSON format | 4j8b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j8/4j8b ftp://data.pdbj.org/pub/pdb/validation_reports/j8/4j8b | HTTPS FTP |
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-Related structure data
Related structure data | 4j73C 4j77C 4j78C 4j79C 4j81C 4j82C 4j84C 4j86C 4j87C 4j8gC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 37525.863 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Schizosaccharomyces pombe (fission yeast) / References: UniProt: Q96WV5 |
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#2: Protein/peptide | Mass: 716.952 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Saccharomyces cerevisiae (brewer's yeast) |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.09 % |
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-E |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD |
Radiation | Monochromator: Cryogenically-cooled single crystal Si(220) side bounce Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.878→77.105 Å / Num. all: 33643 / Num. obs: 29390 / % possible obs: 98.8 % / Observed criterion σ(I): 2.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.878→59.729 Å / SU ML: 0.18 / σ(F): 1.35 / Phase error: 20.59 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.878→59.729 Å
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Refine LS restraints |
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LS refinement shell |
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