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Open data
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Basic information
Entry | Database: PDB / ID: 4j82 | ||||||
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Title | Crystal structure of beta'-COP/Insig-2 complex | ||||||
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![]() | PROTEIN TRANSPORT / beta propeller domain / dilysine motif / ER retrieval / vesicle trafficking | ||||||
Function / homology | ![]() SREBP-SCAP complex retention in endoplasmic reticulum / SREBP-SCAP-Insig complex / cranial suture morphogenesis / negative regulation of steroid biosynthetic process / COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / COPI vesicle coat / SREBP signaling pathway / late endosome to vacuole transport via multivesicular body sorting pathway / intracellular mRNA localization ...SREBP-SCAP complex retention in endoplasmic reticulum / SREBP-SCAP-Insig complex / cranial suture morphogenesis / negative regulation of steroid biosynthetic process / COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / COPI vesicle coat / SREBP signaling pathway / late endosome to vacuole transport via multivesicular body sorting pathway / intracellular mRNA localization / negative regulation of fatty acid biosynthetic process / intra-Golgi vesicle-mediated transport / Regulation of cholesterol biosynthesis by SREBP (SREBF) / oxysterol binding / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / middle ear morphogenesis / inner ear morphogenesis / triglyceride metabolic process / roof of mouth development / cholesterol biosynthetic process / endoplasmic reticulum to Golgi vesicle-mediated transport / protein sequestering activity / ubiquitin binding / intracellular protein transport / cellular response to insulin stimulus / Golgi membrane / endoplasmic reticulum membrane / structural molecule activity / endoplasmic reticulum Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ma, W. / Goldberg, J. | ||||||
![]() | ![]() Title: Rules for the recognition of dilysine retrieval motifs by coatomer. Authors: Ma, W. / Goldberg, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 258.7 KB | Display | ![]() |
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PDB format | ![]() | 210.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4j73C ![]() 4j77C ![]() 4j78C ![]() 4j79C ![]() 4j81C ![]() 4j84C ![]() 4j86C ![]() 4j87C ![]() 4j8bC ![]() 4j8gC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34293.652 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Protein/peptide | Mass: 629.662 Da / Num. of mol.: 2 / Fragment: UNP residues 22-225 / Source method: obtained synthetically / Source: (synth.) ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.07 % |
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD |
Radiation | Monochromator: Cryogenically-cooled single crystal Si(220) side bounce Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.461→76.01 Å / Num. all: 157300 / Num. obs: 102387 / % possible obs: 95.9 % / Observed criterion σ(I): 2.2 |
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Processing
Software | Name: PHENIX / Version: (phenix.refine: 1.7.3_928) / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.98 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 55.592 Å2 / ksol: 0.389 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.461→43.674 Å
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Refine LS restraints |
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LS refinement shell |
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