+Open data
-Basic information
Entry | Database: PDB / ID: 4j79 | ||||||
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Title | Crystal structure of beta'-COP/PEDVspike complex | ||||||
Components |
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Keywords | PROTEIN TRANSPORT / SEC27 / Beta propeller domain / ER retrieval signal | ||||||
Function / homology | Function and homology information COPI vesicle coat / COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / intracellular mRNA localization / late endosome to vacuole transport via multivesicular body sorting pathway / intra-Golgi vesicle-mediated transport / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / endoplasmic reticulum to Golgi vesicle-mediated transport / ubiquitin binding / intracellular protein transport ...COPI vesicle coat / COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / intracellular mRNA localization / late endosome to vacuole transport via multivesicular body sorting pathway / intra-Golgi vesicle-mediated transport / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / endoplasmic reticulum to Golgi vesicle-mediated transport / ubiquitin binding / intracellular protein transport / endocytosis involved in viral entry into host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated virion attachment to host cell / fusion of virus membrane with host plasma membrane / Golgi membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion membrane / structural molecule activity / membrane Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) Porcine epidemic diarrhea virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.559 Å | ||||||
Authors | Ma, W. / Goldberg, J. | ||||||
Citation | Journal: Embo J. / Year: 2013 Title: Rules for the recognition of dilysine retrieval motifs by coatomer. Authors: Ma, W. / Goldberg, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4j79.cif.gz | 81.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4j79.ent.gz | 61 KB | Display | PDB format |
PDBx/mmJSON format | 4j79.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j7/4j79 ftp://data.pdbj.org/pub/pdb/validation_reports/j7/4j79 | HTTPS FTP |
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-Related structure data
Related structure data | 4j73C 4j77C 4j78C 4j81C 4j82C 4j84C 4j86C 4j87C 4j8bC 4j8gC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 34307.680 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P41811 |
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#2: Protein/peptide | Mass: 740.846 Da / Num. of mol.: 1 / Fragment: UNP residues 1378-1383 / Source method: obtained synthetically / Source: (synth.) Porcine epidemic diarrhea virus / References: UniProt: Q91AV1 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.25 % |
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-E |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD |
Radiation | Monochromator: Cryogenically-cooled single crystal Si(220) side bounce Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.559→83.465 Å / Num. obs: 43962 / % possible obs: 97.2 % / Observed criterion σ(I): 13.3 |
-Processing
Software | Name: PHENIX / Version: (phenix.refine: 1.8_1069) / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.559→42.006 Å / SU ML: 0.15 / σ(F): 1.35 / Phase error: 21.93 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.559→42.006 Å
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Refine LS restraints |
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LS refinement shell |
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