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- PDB-3uvl: Crystal structure of WDR5 in complex with the WDR5-interacting mo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3uvl | ||||||
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Title | Crystal structure of WDR5 in complex with the WDR5-interacting motif of MLL3 | ||||||
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![]() | TRANSCRIPTION / trithorax / chromatin biology / beta-propeller / scaffolding / histone H3 / nucleus | ||||||
Function / homology | ![]() [histone H3]-lysine4 N-methyltransferase / histone H3K4 monomethyltransferase activity / histone H3K4 trimethyltransferase activity / MLL3/4 complex / Set1C/COMPASS complex / MLL1/2 complex / ATAC complex / NSL complex / histone H3K4 methyltransferase activity / Cardiogenesis ...[histone H3]-lysine4 N-methyltransferase / histone H3K4 monomethyltransferase activity / histone H3K4 trimethyltransferase activity / MLL3/4 complex / Set1C/COMPASS complex / MLL1/2 complex / ATAC complex / NSL complex / histone H3K4 methyltransferase activity / Cardiogenesis / histone methyltransferase complex / regulation of tubulin deacetylation / acyltransferase activity / histone methyltransferase activity / Formation of WDR5-containing histone-modifying complexes / regulation of cell division / regulation of embryonic development / MLL1 complex / transcription factor TFIID complex / RNA polymerase II general transcription initiation factor activity / histone acetyltransferase complex / response to electrical stimulus / positive regulation of gluconeogenesis / transcription initiation-coupled chromatin remodeling / methylated histone binding / skeletal system development / gluconeogenesis / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / mitotic spindle / PKMTs methylate histone lysines / RMTs methylate histone arginines / Activation of anterior HOX genes in hindbrain development during early embryogenesis / Neddylation / HATs acetylate histones / histone binding / methylation / transcription coactivator activity / regulation of cell cycle / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / RNA binding / nucleoplasm / nucleus / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, P. / Lee, H. / Brunzelle, J.S. / Couture, J.-F. | ||||||
![]() | ![]() Title: The plasticity of WDR5 peptide-binding cleft enables the binding of the SET1 family of histone methyltransferases. Authors: Zhang, P. / Lee, H. / Brunzelle, J.S. / Couture, J.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 134.3 KB | Display | ![]() |
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PDB format | ![]() | 103.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 422 KB | Display | ![]() |
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Full document | ![]() | 423.8 KB | Display | |
Data in XML | ![]() | 14.7 KB | Display | |
Data in CIF | ![]() | 20.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3uvkC ![]() 3uvmC ![]() 3uvnC ![]() 3uvoC ![]() 2h13S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34794.449 Da / Num. of mol.: 1 / Fragment: UNP residues 21-334 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1106.254 Da / Num. of mol.: 1 / Fragment: WDR5-interacting motif (UNP residues 4707-4717) / Mutation: C4708S,S4711A / Source method: obtained synthetically / Source: (synth.) ![]() References: UniProt: Q8NEZ4, histone-lysine N-methyltransferase |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 37.79 % |
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Crystal grow | Temperature: 323 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 0.1 M ammonium acetate, 25% PEG3350, 0.1 M Bis-Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 323K |
-Data collection
Diffraction | Mean temperature: 200 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jun 10, 2011 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.12718 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→54 Å / Num. all: 15066 / Num. obs: 14659 / % possible obs: 97.3 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 7.1 % / Biso Wilson estimate: 41.96 Å2 / Rsym value: 0.075 / Net I/σ(I): 41 |
Reflection shell | Resolution: 2.2→2.25 Å / Redundancy: 5.3 % / Mean I/σ(I) obs: 8.5 / Rsym value: 0.165 / % possible all: 81.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2H13 Resolution: 2.2→40.49 Å / Cor.coef. Fo:Fc: 0.9594 / Cor.coef. Fo:Fc free: 0.9275 / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 39.03 Å2
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Refine analyze | Luzzati coordinate error obs: 0.232 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→40.49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.38 Å / Total num. of bins used: 7
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Refinement TLS params. | Method: refined / Origin x: -12.5935 Å / Origin y: 23.3525 Å / Origin z: -8.3531 Å
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Refinement TLS group | Selection details: { A|31 - A|334 } |