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Open data
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Basic information
Entry | Database: PDB / ID: 4h0g | ||||||
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Title | Crystal structure of mimicry-recognizing native 2D10 scFv | ||||||
![]() | 2D10 scFv | ||||||
![]() | IMMUNE SYSTEM / molecular mimicry / antigen binding / sugar | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Tapryal, S. / Gaur, V. / Kaur, K.J. / Salunke, D.M. | ||||||
![]() | ![]() Title: Structural evaluation of a mimicry-recognizing paratope: plasticity in antigen-antibody interactions manifests in molecular mimicry. Authors: Tapryal, S. / Gaur, V. / Kaur, K.J. / Salunke, D.M. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63.6 KB | Display | ![]() |
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PDB format | ![]() | 46.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.5 KB | Display | ![]() |
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Full document | ![]() | 449.4 KB | Display | |
Data in XML | ![]() | 14.7 KB | Display | |
Data in CIF | ![]() | 20.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4h0hSC ![]() 4h0iC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 26894.973 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-TRS / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.44 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 50 mM MES, pH 6.5, 1.6 M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Apr 9, 2009 / Details: mirrors |
Radiation | Monochromator: Ni filter CMF 12 38CU-6 (Osmic Inc.) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→35 Å / Num. all: 12529 / Num. obs: 12216 / % possible obs: 100 % / Redundancy: 14.1 % / Rmerge(I) obs: 0.077 / Net I/σ(I): 26.3 |
Reflection shell | Resolution: 2.2→2.32 Å / Redundancy: 14 % / Rmerge(I) obs: 0.597 / Mean I/σ(I) obs: 4.6 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4H0H Resolution: 2.3→35 Å / Occupancy max: 1 / Occupancy min: 1 / σ(F): 0
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Solvent computation | Bsol: 71.3332 Å2 | ||||||||||||||||||||||||
Displacement parameters | Biso max: 136.55 Å2 / Biso mean: 44.5573 Å2 / Biso min: 14.09 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→35 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.25 Å
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Xplor file |
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