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- PDB-4eyn: Human Insulin -

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Basic information

Entry
Database: PDB / ID: 4eyn
TitleHuman Insulin
Components
  • Insulin A chain
  • Insulin B chain
KeywordsHORMONE
Function / homology
Function and homology information


negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / negative regulation of NAD(P)H oxidase activity / Insulin processing / nitric oxide-cGMP-mediated signaling pathway / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / regulation of protein secretion ...negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / negative regulation of NAD(P)H oxidase activity / Insulin processing / nitric oxide-cGMP-mediated signaling pathway / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / regulation of protein secretion / positive regulation of peptide hormone secretion / Insulin receptor recycling / Regulation of gene expression in beta cells / positive regulation of respiratory burst / alpha-beta T cell activation / negative regulation of respiratory burst involved in inflammatory response / negative regulation of acute inflammatory response / positive regulation of dendritic spine maintenance / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of glycogen biosynthetic process / negative regulation of reactive oxygen species biosynthetic process / negative regulation of protein secretion / regulation of protein localization to plasma membrane / COPI-mediated anterograde transport / negative regulation of gluconeogenesis / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of lipid catabolic process / positive regulation of nitric oxide mediated signal transduction / positive regulation of lipid biosynthetic process / fatty acid homeostasis / positive regulation of insulin receptor signaling pathway / transport vesicle / insulin-like growth factor receptor binding / neuron projection maintenance / positive regulation of protein autophosphorylation / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of protein metabolic process / Regulation of insulin secretion / NPAS4 regulates expression of target genes / Insulin receptor signalling cascade / positive regulation of glycolytic process / positive regulation of brown fat cell differentiation / positive regulation of mitotic nuclear division / positive regulation of long-term synaptic potentiation / endosome lumen / positive regulation of cytokine production / activation of protein kinase B activity / regulation of synaptic plasticity / regulation of transmembrane transporter activity / positive regulation of cell differentiation / positive regulation of protein secretion / acute-phase response / positive regulation of glucose import / cognition / negative regulation of proteolysis / positive regulation of nitric-oxide synthase activity / hormone activity / vasodilation / insulin receptor binding / negative regulation of protein catabolic process / positive regulation of protein localization to nucleus / Golgi lumen / wound healing / regulation of protein localization / glucose metabolic process / cell-cell signaling / insulin receptor signaling pathway / glucose homeostasis / positive regulation of phosphatidylinositol 3-kinase signaling / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / positive regulation of protein kinase B signaling / positive regulation of MAPK cascade / protease binding / positive regulation of NF-kappaB transcription factor activity / G protein-coupled receptor signaling pathway / positive regulation of cell migration / Amyloid fiber formation / Golgi membrane / endoplasmic reticulum lumen / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding
Similarity search - Function
Insulin / Insulin family / Insulin-like / Insulin/IGF/Relaxin family / Insulin / insulin-like growth factor / relaxin family. / Insulin, conserved site / Insulin-like superfamily / Insulin family signature.
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.532 Å
AuthorsFavero-Retto, M.P. / Palmieri, L.C. / Lima, L.M.T.R.
CitationJournal: Eur J Pharm Biopharm / Year: 2013
Title: Structural meta-analysis of regular human insulin in pharmaceutical formulations.
Authors: Favero-Retto, M.P. / Palmieri, L.C. / Souza, T.A. / Almeida, F.C. / Lima, L.M.
History
DepositionMay 1, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 1, 2013Provider: repository / Type: Initial release
Revision 1.1Jun 12, 2013Group: Database references
Revision 1.2Dec 18, 2013Group: Database references
Revision 1.3Nov 15, 2017Group: Refinement description / Category: software / Item: _software.name

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Insulin A chain
B: Insulin B chain
C: Insulin A chain
D: Insulin B chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,8378
Polymers11,6354
Non-polymers2024
Water2,252125
1
A: Insulin A chain
B: Insulin B chain
C: Insulin A chain
D: Insulin B chain
hetero molecules

A: Insulin A chain
B: Insulin B chain
C: Insulin A chain
D: Insulin B chain
hetero molecules

A: Insulin A chain
B: Insulin B chain
C: Insulin A chain
D: Insulin B chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)35,51124
Polymers34,90612
Non-polymers60512
Water21612
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_555-y,x-y,z1
crystal symmetry operation3_555-x+y,-x,z1
Buried area22180 Å2
ΔGint-636 kcal/mol
Surface area10220 Å2
MethodPISA
Unit cell
Length a, b, c (Å)80.960, 80.960, 33.170
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number146
Space group name H-MH3
Components on special symmetry positions
IDModelComponents
11B-101-

ZN

21B-102-

CL

31D-242-

HOH

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Components

#1: Protein/peptide Insulin A chain


Mass: 2383.698 Da / Num. of mol.: 2 / Fragment: UNP residues 90-110 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P01308
#2: Protein/peptide Insulin B chain


Mass: 3433.953 Da / Num. of mol.: 2 / Fragment: UNP residues 25-54 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P01308
#3: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#4: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 125 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 1.8 Å3/Da / Density % sol: 31.59 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 5.5
Details: 2 uL 0.1 M sodium phosphate, pH 5.5, 10% w/v PEG6000 + 2 uL 100 U/mL human insulin (Insunorm R, lot # I 550005), cryoprotectant: mother liquor + 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K

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Data collection

Diffraction sourceSource: SYNCHROTRON / Site: LNLS / Beamline: D03B-MX1
DetectorType: MAR CCD 165 mm / Detector: CCD
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthRelative weight: 1
ReflectionResolution: 1.532→40.48 Å / Num. all: 11111 / Num. obs: 11111 / % possible obs: 91.1 % / Redundancy: 3 % / Rsym value: 0.06 / Net I/σ(I): 12.4
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured allNum. unique allRsym value% possible all
1.532-1.612.90.3971.5512517830.397100
1.61-1.712.90.2272.7487416860.227100
1.71-1.8330.1085.3431814480.10891.7
1.83-1.9830.133333811060.1375.2
1.98-2.173.10.0688373112210.06889.8
2.17-2.423.10.1093.1329410670.10987
2.42-2.83.10.03816.630609750.03889.7
2.8-3.433.20.03516.228258930.03598
3.43-4.843.20.03416.618045610.03479.4
4.84-15.4983.20.02723.411823710.02796.8

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Phasing

PhasingMethod: molecular replacement
Phasing MRRfactor: 27.43 / Model details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation2.5 Å15.5 Å
Translation2.5 Å15.5 Å

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Processing

Software
NameVersionClassificationNB
MOSFLMdata reduction
SCALA3.3.15data scaling
PHASER2.1.4phasing
REFMAC5.6.0117refinement
PDB_EXTRACT3.11data extraction
MAR345data collection
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.532→15.5 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.908 / Occupancy max: 1 / Occupancy min: 0.25 / SU B: 5.499 / SU ML: 0.094 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.216 / ESU R Free: 0.133 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
RfactorNum. reflection% reflectionSelection details
Rfree0.2709 535 4.8 %RANDOM
Rwork0.173 ---
obs0.1777 11107 91.13 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK
Displacement parametersBiso max: 69.69 Å2 / Biso mean: 24.5619 Å2 / Biso min: 4.58 Å2
Baniso -1Baniso -2Baniso -3
1--0.03 Å2-0.01 Å20 Å2
2---0.03 Å20 Å2
3---0.04 Å2
Refinement stepCycle: LAST / Resolution: 1.532→15.5 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms809 0 4 125 938
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0190.02895
X-RAY DIFFRACTIONr_bond_other_d0.0020.02581
X-RAY DIFFRACTIONr_angle_refined_deg1.9061.9521227
X-RAY DIFFRACTIONr_angle_other_deg1.0533.0171405
X-RAY DIFFRACTIONr_dihedral_angle_1_deg7.235112
X-RAY DIFFRACTIONr_dihedral_angle_2_deg35.05224.04842
X-RAY DIFFRACTIONr_dihedral_angle_3_deg13.09615140
X-RAY DIFFRACTIONr_dihedral_angle_4_deg13.516153
X-RAY DIFFRACTIONr_chiral_restr0.1340.2134
X-RAY DIFFRACTIONr_gen_planes_refined0.0090.021031
X-RAY DIFFRACTIONr_gen_planes_other0.0020.02196
X-RAY DIFFRACTIONr_rigid_bond_restr5.18731472
X-RAY DIFFRACTIONr_sphericity_free34.647530
X-RAY DIFFRACTIONr_sphericity_bonded11.37851541
LS refinement shellResolution: 1.532→1.571 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.331 43 -
Rwork0.215 870 -
all-913 -
obs--100 %

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