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- PDB-4f4v: Human Insulin -

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Basic information

Entry
Database: PDB / ID: 4f4v
TitleHuman Insulin
Components
  • Insulin A chain
  • Insulin B chain
KeywordsHORMONE / pancreatic hormone
Function / homology
Function and homology information


Synthesis, secretion, and deacylation of Ghrelin / Signaling by Insulin receptor / COPI-mediated anterograde transport / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / IRS activation / Signal attenuation / Insulin receptor signalling cascade / Regulation of insulin secretion / Insulin receptor recycling / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes ...Synthesis, secretion, and deacylation of Ghrelin / Signaling by Insulin receptor / COPI-mediated anterograde transport / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / IRS activation / Signal attenuation / Insulin receptor signalling cascade / Regulation of insulin secretion / Insulin receptor recycling / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / Amyloid fiber formation / Insulin processing / Regulation of gene expression in beta cells / negative regulation of glycogen catabolic process / alpha-beta T cell activation / negative regulation of fatty acid metabolic process / negative regulation of NAD(P)H oxidase activity / negative regulation of feeding behavior / positive regulation of respiratory burst / regulation of protein secretion / positive regulation of peptide hormone secretion / negative regulation of respiratory burst involved in inflammatory response / negative regulation of blood vessel diameter / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of dendritic spine maintenance / positive regulation of lipid biosynthetic process / regulation of transmembrane transporter activity / negative regulation of gluconeogenesis / negative regulation of reactive oxygen species biosynthetic process / negative regulation of lipid catabolic process / negative regulation of protein secretion / positive regulation of cellular protein metabolic process / negative regulation of protein oligomerization / fatty acid homeostasis / regulation of protein localization to plasma membrane / positive regulation of glycogen biosynthetic process / positive regulation of nitric oxide mediated signal transduction / regulation of cellular amino acid metabolic process / transport vesicle / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of brown fat cell differentiation / regulation of synaptic plasticity / endosome lumen / cognition / neuron projection maintenance / positive regulation of insulin receptor signaling pathway / insulin-like growth factor receptor binding / positive regulation of cytokine secretion / positive regulation of protein autophosphorylation / positive regulation of mitotic nuclear division / negative regulation of acute inflammatory response / positive regulation of glycolytic process / positive regulation of cell differentiation / regulation of protein localization / positive regulation of long-term synaptic potentiation / activation of protein kinase B activity / positive regulation of glucose import / positive regulation of blood vessel diameter / negative regulation of protein catabolic process / acute-phase response / hormone activity / positive regulation of nitric-oxide synthase activity / negative regulation of proteolysis / positive regulation of protein localization to nucleus / insulin receptor binding / insulin receptor signaling pathway / glucose metabolic process / cell-cell signaling / Golgi lumen / wound healing / glucose homeostasis / endoplasmic reticulum to Golgi vesicle-mediated transport / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase signaling / positive regulation of cell growth / secretory granule lumen / positive regulation of NF-kappaB transcription factor activity / protease binding / positive regulation of cell migration / positive regulation of protein kinase B signaling / Golgi membrane / G protein-coupled receptor signaling pathway / endoplasmic reticulum lumen / regulation of transcription, DNA-templated / cellular protein metabolic process / positive regulation of gene expression / positive regulation of cell population proliferation / extracellular space / extracellular region / identical protein binding
Insulin / Insulin-like / Insulin family / Insulin, conserved site / Insulin-like superfamily / Insulin/IGF/Relaxin family / Insulin family signature.
Insulin
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.637 Å
AuthorsFavero-Retto, M.P. / Palmieri, L.C. / Lima, L.M.T.R.
CitationJournal: Eur J Pharm Biopharm / Year: 2013
Title: Structural meta-analysis of regular human insulin in pharmaceutical formulations.
Authors: Favero-Retto, M.P. / Palmieri, L.C. / Souza, T.A. / Almeida, F.C. / Lima, L.M.
Validation Report
SummaryFull reportAbout validation report
History
DepositionMay 11, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 15, 2013Provider: repository / Type: Initial release
Revision 1.1Jun 12, 2013Group: Database references
Revision 1.2Dec 18, 2013Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Insulin A chain
B: Insulin B chain
C: Insulin A chain
D: Insulin B chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,8378
Polymers11,6354
Non-polymers2024
Water1,820101
1
A: Insulin A chain
B: Insulin B chain
C: Insulin A chain
D: Insulin B chain
hetero molecules

A: Insulin A chain
B: Insulin B chain
C: Insulin A chain
D: Insulin B chain
hetero molecules

A: Insulin A chain
B: Insulin B chain
C: Insulin A chain
D: Insulin B chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)35,51124
Polymers34,90612
Non-polymers60512
Water21612
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_555-y,x-y,z1
crystal symmetry operation3_555-x+y,-x,z1
Buried area22710 Å2
ΔGint-713 kcal/mol
Surface area10150 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)81.970, 81.970, 33.690
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number146
Space group name H-MH3
Components on special symmetry positions
IDModelComponents
11B-101-

ZN

21B-102-

CL

31D-101-

ZN

41D-102-

CL

51D-228-

HOH

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Components

#1: Protein/peptide Insulin A chain


Mass: 2383.698 Da / Num. of mol.: 2 / Fragment: UNP residues 90-110 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P01308
#2: Protein/peptide Insulin B chain


Mass: 3433.953 Da / Num. of mol.: 2 / Fragment: UNP residues 25-54 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P01308
#3: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn / Zinc
#4: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl / Chloride
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 101 / Source method: isolated from a natural source / Formula: H2O / Water

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 1.87 Å3/Da / Density % sol: 34.3 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5
Details: 2 uL 0.1 M sodium phosphate, pH 5.5, 10% w/v PEG6000 + 2 uL 100 U/mL human insulin (Humulin R, lot # A 505073), cryoprotectant: mother liquor + 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: LNLS / Beamline: W01B-MX2 / Wavelength: 1.4586 Å
DetectorType: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 1, 2010
RadiationMonochromator: double flat crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.4586 Å / Relative weight: 1
ReflectionResolution: 1.637→24.435 Å / Num. all: 9124 / Num. obs: 9124 / % possible obs: 87.8 % / Redundancy: 2.8 % / Rmerge(I) obs: 0.151 / Rsym value: 0.151 / Net I/σ(I): 6.3
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured allNum. unique allRsym value% possible all
1.637-1.732.40.6820.5341414200.68294.7
1.73-1.832.70.4911.3362913690.49195.2
1.83-1.962.70.4930.526249790.49371.7
1.96-2.112.90.3850.4306310720.38584.6
2.11-2.322.90.2171.528909860.21786.5
2.32-2.5930.153.8311810270.1598.3
2.59-2.993.10.1443.924727940.14486.4
2.99-3.663.10.1343.121566900.13488.2
3.66-5.183.30.083715844870.08381.5
5.18-24.4353.40.0688.510083000.06889.6

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Phasing

PhasingMethod: molecular replacement
Phasing MRRfactor: 32.59 / Model details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation2.5 Å24.44 Å
Translation2.5 Å24.44 Å

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Processing

Software
NameVersionClassificationNB
MOSFLMdata reduction
SCALA3.2.25data scaling
PHASER2.1.4phasing
REFMAC5.5.0102refinement
PDB_EXTRACT3.11data extraction
MAR345dtbdata collection
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.637→24.435 Å / Cor.coef. Fo:Fc: 0.936 / Cor.coef. Fo:Fc free: 0.913 / Occupancy max: 1 / Occupancy min: 0.33 / SU B: 9.366 / SU ML: 0.129 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.157 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
RfactorNum. reflection% reflectionSelection details
Rfree0.2638 446 5 %RANDOM
Rwork0.209 ---
Obs0.2118 9005 86.6 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK
Displacement parametersBiso max: 79.21 Å2 / Biso mean: 20.8177 Å2 / Biso min: 7.33 Å2
Baniso -1Baniso -2Baniso -3
1--0.3 Å2-0.15 Å20 Å2
2---0.3 Å20 Å2
3---0.45 Å2
Refinement stepCycle: LAST / Resolution: 1.637→24.435 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms808 0 4 101 913
Refine LS restraints

Refinement-ID: X-RAY DIFFRACTION

TypeDev idealDev ideal targetNumber
r_bond_refined_d0.0260.022894
r_bond_other_d0.0040.02568
r_angle_refined_deg2.0541.9471224
r_angle_other_deg1.16831390
r_dihedral_angle_1_deg8.1415111
r_dihedral_angle_2_deg33.90124.04842
r_dihedral_angle_3_deg13.78215140
r_dihedral_angle_4_deg8.952153
r_chiral_restr0.1350.2135
r_gen_planes_refined0.010.021020
r_gen_planes_other0.0020.02195
r_mcbond_it1.9761.5543
r_mcbond_other0.7161.5220
r_mcangle_it2.7522885
r_scbond_it4.6863351
r_scangle_it6.1714.5339
r_rigid_bond_restr2.06231462
LS refinement shellResolution: 1.637→1.679 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.806 30 -
Rwork0.529 668 -
All-698 -
Obs--91.12 %

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