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- PDB-4cij: Structure of Rolling Circle Replication Initiator Protein from Ge... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4cij | ||||||
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Title | Structure of Rolling Circle Replication Initiator Protein from Geobacillus stearothermophilus. | ||||||
![]() | GST REP | ||||||
![]() | ISOMERASE / CELL CYCLE / ROLLING CIRCLE REPLICATION / ANTIBIOTIC RESISTANCE / TYPE I TOPOISOMERASE | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Carr, S.B. / Phillips, S.E.V. / Thomas, C.D. | ||||||
![]() | ![]() Title: Structures of Replication Initiation Proteins from Staphylococcal Antibiotic Resistance Plasmids Reveal Protein Asymmetry and Flexibility are Necessary for Replication. Authors: Carr, S.B. / Phillips, S.E. / Thomas, C.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 437.1 KB | Display | ![]() |
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PDB format | ![]() | 365.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 445.1 KB | Display | ![]() |
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Full document | ![]() | 452.1 KB | Display | |
Data in XML | ![]() | 39.1 KB | Display | |
Data in CIF | ![]() | 54.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 31378.123 Da / Num. of mol.: 4 / Fragment: DNA RELAXASE DOMAIN, RESIDUES 2-271 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: TK015 / Description: NARUMI ET AL. BIOTECH LETT 15, 815-820 / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | Sequence details | PROTEIN CORRESPOND | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.3 % / Description: NONE |
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Crystal grow | pH: 7.5 Details: 0.2 M PROLINE, 0.1 M HEPES PH 7.5, 10 % (V/V) PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Jul 30, 2012 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9163 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→40.2 Å / Num. obs: 60795 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 7 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 16.9 |
Reflection shell | Resolution: 2.3→2.36 Å / Redundancy: 7.1 % / Rmerge(I) obs: 0.86 / Mean I/σ(I) obs: 2.2 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 2.3→40.25 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.944 / SU B: 13.812 / SU ML: 0.161 / Cross valid method: THROUGHOUT / ESU R: 0.289 / ESU R Free: 0.216 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 67.222 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→40.25 Å
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Refine LS restraints |
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