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- PDB-4ct4: CNOT1 MIF4G domain - DDX6 complex -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 4ct4
TitleCNOT1 MIF4G domain - DDX6 complex
Components
  • CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1
  • PROBABLE ATP-DEPENDENT RNA HELICASE DDX6
KeywordsRNA BINDING PROTEIN / DEADENYLATION / TRANSCRIPTION
Function / homology
Function and homology information


positive regulation of cytoplasmic mRNA processing body assembly / CCR4-NOT core complex / armadillo repeat domain binding / CCR4-NOT complex / regulation of stem cell population maintenance / mRNA decay by 5' to 3' exoribonuclease / negative regulation of retinoic acid receptor signaling pathway / positive regulation of mRNA catabolic process / nuclear-transcribed mRNA poly(A) tail shortening / negative regulation of intracellular estrogen receptor signaling pathway ...positive regulation of cytoplasmic mRNA processing body assembly / CCR4-NOT core complex / armadillo repeat domain binding / CCR4-NOT complex / regulation of stem cell population maintenance / mRNA decay by 5' to 3' exoribonuclease / negative regulation of retinoic acid receptor signaling pathway / positive regulation of mRNA catabolic process / nuclear-transcribed mRNA poly(A) tail shortening / negative regulation of intracellular estrogen receptor signaling pathway / trophectodermal cell differentiation / viral RNA genome packaging / miRNA-mediated post-transcriptional gene silencing / Deadenylation of mRNA / P-body assembly / miRNA-mediated gene silencing by inhibition of translation / nuclear retinoic acid receptor binding / M-decay: degradation of maternal mRNAs by maternally stored factors / RISC complex / peroxisomal membrane / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / stem cell population maintenance / negative regulation of neuron differentiation / stress granule assembly / helicase activity / nuclear estrogen receptor binding / P-body / cytoplasmic ribonucleoprotein granule / neuron differentiation / cytoplasmic stress granule / negative regulation of translation / RNA helicase activity / molecular adaptor activity / RNA helicase / cadherin binding / protein domain specific binding / mRNA binding / negative regulation of transcription by RNA polymerase II / ATP hydrolysis activity / extracellular space / RNA binding / ATP binding / membrane / nucleus / cytosol / cytoplasm
Similarity search - Function
CCR4-Not complex component, Not1, C-terminal / CCR4-NOT transcription complex subunit 1, domain 4 / CCR4-NOT transcription complex subunit 1, CAF1-binding domain / CCR4-NOT transcription complex subunit 1, TTP binding domain / CCR4-NOT transcription complex subunit 1, HEAT repeat / CCR4-NOT subunit 1, TTP binding domain superfamily / CCR4-NOT transcription complex subunit 1 / CCR4-Not complex component, Not1 / CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819 / CCR4-NOT transcription complex subunit 1 CAF1-binding domain ...CCR4-Not complex component, Not1, C-terminal / CCR4-NOT transcription complex subunit 1, domain 4 / CCR4-NOT transcription complex subunit 1, CAF1-binding domain / CCR4-NOT transcription complex subunit 1, TTP binding domain / CCR4-NOT transcription complex subunit 1, HEAT repeat / CCR4-NOT subunit 1, TTP binding domain superfamily / CCR4-NOT transcription complex subunit 1 / CCR4-Not complex component, Not1 / CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819 / CCR4-NOT transcription complex subunit 1 CAF1-binding domain / CCR4-NOT transcription complex subunit 1 TTP binding domain / CCR4-NOT transcription complex subunit 1 HEAT repeat / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat - #180 / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site / RNA helicase, DEAD-box type, Q motif / DEAD-box RNA helicase Q motif profile. / DEAD/DEAH box helicase / DEAD/DEAH box helicase domain / Helicase conserved C-terminal domain / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
CCR4-NOT transcription complex subunit 1 / Probable ATP-dependent RNA helicase DDX6
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å
AuthorsOzgur, S. / Basquin, J. / Conti, E.
CitationJournal: Mol.Cell / Year: 2014
Title: Structural and Biochemical Insights to the Role of the Ccr4- not Complex and Ddx6 ATPase in Microrna Repression.
Authors: Mathys, H. / Basquin, J. / Ozgur, S. / Czarnocki-Cieciura, M. / Bonneau, F. / Aartse, A. / Dziembowski, A. / Nowotny, M. / Conti, E. / Filipowicz, W.
History
DepositionMar 12, 2014Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 7, 2014Provider: repository / Type: Initial release
Revision 1.1Jun 18, 2014Group: Database references
Revision 1.2Dec 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Other / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_initial_refinement_model / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1
B: PROBABLE ATP-DEPENDENT RNA HELICASE DDX6
C: CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1
D: PROBABLE ATP-DEPENDENT RNA HELICASE DDX6
hetero molecules


Theoretical massNumber of molelcules
Total (without water)145,9029
Polymers145,7694
Non-polymers1335
Water4,828268
1
A: CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1
B: PROBABLE ATP-DEPENDENT RNA HELICASE DDX6
hetero molecules


Theoretical massNumber of molelcules
Total (without water)72,9575
Polymers72,8852
Non-polymers733
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2630 Å2
ΔGint-7.8 kcal/mol
Surface area33100 Å2
MethodPISA
2
C: CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1
D: PROBABLE ATP-DEPENDENT RNA HELICASE DDX6
hetero molecules


Theoretical massNumber of molelcules
Total (without water)72,9444
Polymers72,8852
Non-polymers602
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2920 Å2
ΔGint-25.7 kcal/mol
Surface area27270 Å2
MethodPISA
Unit cell
Length a, b, c (Å)71.531, 120.014, 78.469
Angle α, β, γ (deg.)90.00, 104.01, 90.00
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
22
/ NCS ensembles :
ID
1
2

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Components

#1: Protein CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1 / CCR4-ASSOCIATED FACTOR 1 / NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 1 HOMOLOG / NOT1H / HNOT1


Mass: 29695.352 Da / Num. of mol.: 2 / Fragment: MIF4G DOMAIN, RESIDUES 1063-1314
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PEC_HIS_SUMO / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): PRARE / References: UniProt: A5YKK6
#2: Protein PROBABLE ATP-DEPENDENT RNA HELICASE DDX6 / ATP-DEPENDENT RNA HELICASE P54 / DEAD BOX PROTEIN 6 / ONCOGENE RCK / DDX6


Mass: 43189.195 Da / Num. of mol.: 2 / Fragment: RECA1 AND RECA2, RESIDUES 95-469
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PEC_HIS_SUMO / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): PRARE / References: UniProt: P26196
#3: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#4: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 268 / Source method: isolated from a natural source / Formula: H2O
Sequence detailsFIRST 6 AMINO ACIDS (QGPDSM) COMING FROM REMAINING TAG CLEAVAGE FIRST 3 AMINO ACIDS (RSM) COMING ...FIRST 6 AMINO ACIDS (QGPDSM) COMING FROM REMAINING TAG CLEAVAGE FIRST 3 AMINO ACIDS (RSM) COMING FROM REMAINING TAG CLEAVAGE

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.3 Å3/Da / Density % sol: 46 % / Description: NONE
Crystal growpH: 6.5 / Details: 100 MM MES PH 6.5, 300 MM MGCL2

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 7, 2013 / Details: MIRRORS
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.29→76.15 Å / Num. obs: 66414 / % possible obs: 98.9 % / Observed criterion σ(I): 2.2 / Redundancy: 4.7 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 11.6
Reflection shellResolution: 2.29→2.41 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.66 / Mean I/σ(I) obs: 2.2 / % possible all: 93

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE)refinement
XDSdata reduction
SCALAdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 4GML AND PDB ENTRY 2WAX

4gml

2wax


Resolution: 2.3→64.29 Å / SU ML: 0.3 / σ(F): 1.28 / Phase error: 23.91 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2198 5578 5 %
Rwork0.166 --
obs0.1687 55957 99.48 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.3→64.29 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9653 0 5 268 9926
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0089822
X-RAY DIFFRACTIONf_angle_d1.18513296
X-RAY DIFFRACTIONf_dihedral_angle_d12.8493685
X-RAY DIFFRACTIONf_chiral_restr0.0771568
X-RAY DIFFRACTIONf_plane_restr0.0061688
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3-2.32620.31681850.26483556X-RAY DIFFRACTION100
2.3262-2.35350.33681830.27263507X-RAY DIFFRACTION100
2.3535-2.38220.33921910.25793650X-RAY DIFFRACTION100
2.3822-2.41240.33261800.24783439X-RAY DIFFRACTION100
2.4124-2.44410.27911900.23863599X-RAY DIFFRACTION100
2.4441-2.47760.29851840.23283566X-RAY DIFFRACTION99
2.4776-2.5130.27141880.22943527X-RAY DIFFRACTION100
2.513-2.55050.27111910.23263582X-RAY DIFFRACTION100
2.5505-2.59040.30181810.23273502X-RAY DIFFRACTION99
2.5904-2.63280.2791880.22583569X-RAY DIFFRACTION99
2.6328-2.67820.31211840.22813482X-RAY DIFFRACTION99
2.6782-2.72690.2751860.21723559X-RAY DIFFRACTION99
2.7269-2.77940.27171830.20033511X-RAY DIFFRACTION99
2.7794-2.83610.26651780.20053544X-RAY DIFFRACTION99
2.8361-2.89780.27191900.18163573X-RAY DIFFRACTION100
2.8978-2.96520.24781870.17933558X-RAY DIFFRACTION100
2.9652-3.03940.26331890.18573572X-RAY DIFFRACTION100
3.0394-3.12150.241880.18813591X-RAY DIFFRACTION100
3.1215-3.21340.20461850.19383483X-RAY DIFFRACTION100
3.2134-3.31710.24671890.18083591X-RAY DIFFRACTION100
3.3171-3.43560.25021860.17243576X-RAY DIFFRACTION100
3.4356-3.57320.20181900.16063555X-RAY DIFFRACTION100
3.5732-3.73580.1911870.14263541X-RAY DIFFRACTION100
3.7358-3.93270.15621820.13543519X-RAY DIFFRACTION100
3.9327-4.17910.1711870.1313547X-RAY DIFFRACTION100
4.1791-4.50170.18971870.12253527X-RAY DIFFRACTION99
4.5017-4.95460.17941850.12063533X-RAY DIFFRACTION99
4.9546-5.67110.19421810.14773528X-RAY DIFFRACTION99
5.6711-7.14350.20991870.17553555X-RAY DIFFRACTION99
7.1435-64.31530.17611860.12713494X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.3752-1.3766-1.70285.42646.69268.3064-0.07470.36931.7193-1.1825-0.48720.9146-0.8726-0.78990.47180.58880.0199-0.0130.50340.00661.251313.697323.36184.1328
24.39620.9720.92641.03332.03875.69090.566-0.80091.37131.2218-0.99360.91820.0934-0.2045-0.08960.6549-0.3080.3340.7078-0.49170.865425.179425.351321.0097
30.8294-1.0257-2.33262.03392.57556.44631.0323-1.33041.66381.0022-0.22460.75290.0667-0.77620.19030.5712-0.15570.44170.87-0.60461.465615.023726.700719.7041
41.7087-0.4997-1.96030.630.82742.82120.7366-0.98930.77880.7204-0.74870.9631-0.0428-0.1165-0.02350.5552-0.28050.31020.796-0.31630.704117.999716.092418.6623
54.66261.5751-1.59958.0467-2.71948.09390.1694-0.08480.5523-0.2214-0.10330.7295-0.1752-0.1743-0.00530.2778-0.06710.03350.4516-0.10910.379412.4998.05548.8251
68.49593.0423-0.3447.49743.37812.48880.09271.6946-0.2287-1.006-0.26190.4096-0.7075-0.79580.16480.46420.0531-0.1070.6285-0.13090.41310.3788-2.6618-0.7503
74.1705-1.7661-4.39354.44821.70017.9781-0.0182-0.873-1.04910.4847-0.26960.69180.24650.24980.28340.2705-0.09360.02230.58460.08930.516713.5015-5.95213.8738
82.6076-0.17291.36694.09853.30353.56020.1761.1076-1.7333-1.2289-0.63680.3781-0.3744-0.99780.41970.4227-0.07990.010.7114-0.17470.532720.6023-10.615-1.836
92.16361.7735-3.48667.64022.17239.5632-0.4155-0.4427-1.27620.67140.14370.27820.82210.03060.25890.32580.01440.02770.6030.1620.535819.4488-12.288712.4676
105.62193.8545-6.54287.6633-6.40738.3635-0.40371.619-1.543-0.7920.68182.21611.5514-2.456-0.32340.4841-0.21040.06990.7826-0.31881.50594.5458-14.9181.2087
119.092-2.0934-2.60415.94733.59548.57980.24131.14190.5283-0.7024-0.278-0.1807-0.5625-0.15010.01460.32270.02890.00670.42540.06310.349734.9754-6.5915-2.5097
123.0958-0.3065-0.01381.879-0.10455.4084-0.0632-0.3529-0.06820.20110.1217-0.08950.0303-0.1282-0.0450.27-0.00550.00610.3369-0.03110.261450.6904-8.321910.098
138.19425.46712.17636.6251-1.14986.3888-0.1382-0.5212-0.1951-0.12270.0202-0.5302-0.77241.00150.11670.3645-0.0343-0.00740.5616-0.0970.370565.1821-7.39646.9511
146.37-3.46172.34092.5230.03667.446-0.05890.53490.5274-0.0673-0.1973-0.529-0.10070.65970.26710.3061-0.02470.01830.4188-0.02540.28459.7805-2.9406-2.7135
157.3581-3.81647.19799.1898-6.63168.42670.04550.2711-0.22930.0539-0.2250.5344-0.02140.79430.16640.3978-0.04160.05930.3797-0.05150.27155.5823-3.487-7.8427
164.40922.50530.23456.3068-1.28662.54550.0759-0.16060.76020.0177-0.12880.8297-0.106-0.07010.03860.2818-0.01610.05360.3195-0.09060.403436.058129.61396.571
173.64991.1113-0.34956.3475-0.85471.95250.2878-0.8765-0.09060.7238-0.5507-0.3105-0.06580.66110.24720.4057-0.121-0.02550.7226-0.01670.307844.391921.61618.5084
182.77822.49993.48187.7911-1.66548.76560.22740.3605-1.0687-0.491-0.2333-1.30191.41961.201-0.02650.51880.1260.12070.6027-0.11650.648941.025215.67518.3638
195.2183-1.4335-0.92868.83380.90696.096-0.03470.20060.1092-0.8076-0.2121-0.03910.18160.1280.21710.34370.03450.02990.32060.02230.252442.442928.6068-0.8341
206.1263-3.54471.44456.645-0.16967.6277-0.95790.7171-0.32770.45920.63850.53540.7084-0.46680.39860.80570.04750.14860.84710.45211.251827.426839.88016.6557
213.9583-4.77690.72496.10740.13822.9105-0.4263-1.47190.5136-0.36830.21311.2792-1.2620.28-0.01480.71960.24930.09630.8496-0.080.76757.855513.1693-20.8177
222.4743-1.48260.60275.3917-0.15623.3701-0.0860.03331.15890.40420.1191-0.5403-0.72441.0666-0.06820.6527-0.25740.16670.77940.02680.717812.392921.7126-31.3431
236.10671.96033.53426.78644.50837.7926-0.27092.07522.9641-1.53410.0157-1.0359-1.0971-0.06340.26140.8945-0.13190.22660.76690.25370.75668.860926.7573-43.8307
241.67810.7302-0.57233.876-2.14481.92920.19680.12990.9325-0.3733-0.1517-0.7804-0.64510.88120.01780.6217-0.16470.24790.65330.06960.816113.408917.5861-37.2321
258.6189-0.4752-1.42764.4234-5.60388.47370.3653-0.71720.59180.93770.2054-0.3471-0.86830.532-0.32610.5381-0.10610.02170.8945-0.23220.692920.49757.9349-29.3453
266.671-2.691-0.77.26871.06392.8524-0.1232-0.40390.09350.15150.23-0.6276-0.26691.0564-0.07290.2843-0.06130.03180.71440.02240.411319.35242.0675-36.855
272.8625-1.33461.72198.0523-2.98196.4544-0.2254-0.82190.03860.5065-0.0302-0.6023-0.0258-0.03050.2370.27920.0967-0.02350.81240.0890.403118.2593-7.096-30.4452
283.3508-2.43530.06574.4305-3.73415.19960.3734-0.82230.0842-0.79280.01730.1750.3460.1859-0.37350.50160.0222-0.0130.83250.03970.377618.0595-4.1464-50.3573
294.7199-0.75530.90432.46120.16983.0825-0.3922-0.6701-0.77470.02290.0236-0.14560.88221.0740.30870.39790.21130.06710.71250.17740.449115.9551-14.7956-34.8975
306.2715-2.71596.16226.2815-1.72046.66270.2565-0.026-1.0289-0.2529-0.189-0.33630.94171.0921-0.18270.41280.19490.10040.7280.15910.457218.5735-17.035-38.4627
317.64191.1843-3.02472.1946-0.09333.52480.0894-0.9366-0.03640.1716-0.0944-0.13810.3630.72890.05670.4210.0553-0.02860.47930.07510.2447-7.3534-12.4568-25.1728
328.68252.08961.19416.95254.57894.24650.03250.3021-0.5085-0.2280.2266-0.48110.0186-0.1325-0.17720.48560.0180.1210.37410.12330.2405-14.1773-15.0541-34.3186
333.50512.11412.0690.85030.58436.7341-0.35931.00060.6833-0.4419-0.0180.1776-0.16630.29230.33180.4362-0.0436-0.01520.40190.12880.3667-15.5824-7.2875-42.1073
344.6196-3.42683.45893.9755-3.49423.1632-0.22021.5015-0.3398-1.52410.5815-0.8929-0.55441.3571-0.28110.7529-0.08070.1040.8817-0.08310.3729-20.3611-13.8516-52.9759
354.54910.54320.0112.51631.0258.4631-0.08630.07210.2021-0.0436-0.10530.4850.1841-0.72460.18110.2987-0.04030.01090.28820.00980.307-27.3806-11.2498-35.0616
363.9352.88064.47572.58043.35675.97030.2298-0.64530.27020.0229-0.37310.24610.0525-0.94190.21140.5466-0.06120.21810.5507-0.02610.4748-25.4988-9.0342-19.2452
372.46290.4894-0.13230.97330.55044.0710.07710.22130.6603-0.2536-0.06240.0279-0.93-0.07830.03140.66690.03890.09410.29820.08990.4751-9.808122.2353-30.505
389.1645-4.5999-1.22154.13563.27085.2420.16190.63320.2739-0.19020.1280.2550.0032-0.3856-0.2250.6387-0.01370.08850.43310.14470.3882-10.191213.1702-38.1414
399.5035-5.5782-6.5183.45144.15169.26580.0411-0.3447-0.06250.01520.12960.5347-0.53210.079-0.00370.4079-0.02930.02040.25930.04110.3862-10.161417.0941-25.9248
403.78422.1397-0.33517.4914-0.15098.0284-0.0264-0.61020.10640.55290.01920.2621-0.19890.02330.02220.40110.04220.08230.4372-0.01810.3666-11.75216.8809-16.8796
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(CHAIN A AND RESID 1066:1088)
2X-RAY DIFFRACTION2(CHAIN A AND RESID 1089:1110)
3X-RAY DIFFRACTION3(CHAIN A AND RESID 1111:1129)
4X-RAY DIFFRACTION4(CHAIN A AND RESID 1130:1161)
5X-RAY DIFFRACTION5(CHAIN A AND RESID 1162:1212)
6X-RAY DIFFRACTION6(CHAIN A AND RESID 1213:1229)
7X-RAY DIFFRACTION7(CHAIN A AND RESID 1230:1267)
8X-RAY DIFFRACTION8(CHAIN A AND RESID 1268:1275)
9X-RAY DIFFRACTION9(CHAIN A AND RESID 1276:1294)
10X-RAY DIFFRACTION10(CHAIN A AND RESID 1295:1301)
11X-RAY DIFFRACTION11(CHAIN B AND RESID 92:126)
12X-RAY DIFFRACTION12(CHAIN B AND RESID 127:253)
13X-RAY DIFFRACTION13(CHAIN B AND RESID 254:269)
14X-RAY DIFFRACTION14(CHAIN B AND RESID 270:288)
15X-RAY DIFFRACTION15(CHAIN B AND RESID 289:302)
16X-RAY DIFFRACTION16(CHAIN B AND RESID 303:340)
17X-RAY DIFFRACTION17(CHAIN B AND RESID 341:392)
18X-RAY DIFFRACTION18(CHAIN B AND RESID 393:406)
19X-RAY DIFFRACTION19(CHAIN B AND RESID 407:462)
20X-RAY DIFFRACTION20(CHAIN B AND RESID 463:469)
21X-RAY DIFFRACTION21(CHAIN C AND RESID 1064:1071)
22X-RAY DIFFRACTION22(CHAIN C AND RESID 1072:1107)
23X-RAY DIFFRACTION23(CHAIN C AND RESID 1108:1114)
24X-RAY DIFFRACTION24(CHAIN C AND RESID 1115:1154)
25X-RAY DIFFRACTION25(CHAIN C AND RESID 1155:1181)
26X-RAY DIFFRACTION26(CHAIN C AND RESID 1182:1215)
27X-RAY DIFFRACTION27(CHAIN C AND RESID 1216:1241)
28X-RAY DIFFRACTION28(CHAIN C AND RESID 1242:1251)
29X-RAY DIFFRACTION29(CHAIN C AND RESID 1252:1279)
30X-RAY DIFFRACTION30(CHAIN C AND RESID 1280:1303)
31X-RAY DIFFRACTION31(CHAIN D AND RESID 95:140)
32X-RAY DIFFRACTION32(CHAIN D AND RESID 141:167)
33X-RAY DIFFRACTION33(CHAIN D AND RESID 168:206)
34X-RAY DIFFRACTION34(CHAIN D AND RESID 207:214)
35X-RAY DIFFRACTION35(CHAIN D AND RESID 215:284)
36X-RAY DIFFRACTION36(CHAIN D AND RESID 285:303)
37X-RAY DIFFRACTION37(CHAIN D AND RESID 304:374)
38X-RAY DIFFRACTION38(CHAIN D AND RESID 375:398)
39X-RAY DIFFRACTION39(CHAIN D AND RESID 399:413)
40X-RAY DIFFRACTION40(CHAIN D AND RESID 414:462)

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