+Open data
-Basic information
Entry | Database: PDB / ID: 6lw4 | ||||||
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Title | Structure of Lpg2148/Lpg2149 complex | ||||||
Components |
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Keywords | TRANSFERASE / complex / deamidase / transglutaminase | ||||||
Function / homology | Uncharacterized protein / Uncharacterized protein Function and homology information | ||||||
Biological species | Legionella pneumophila subsp. pneumophila (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||
Authors | Feng, Y. / Wang, Y. / Huang, Y. / Li, D. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Structure of Lpg2148/Lpg2149 complex Authors: Feng, Y. / Wang, Y. / Huang, Y. / Li, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6lw4.cif.gz | 112.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6lw4.ent.gz | 84.9 KB | Display | PDB format |
PDBx/mmJSON format | 6lw4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lw/6lw4 ftp://data.pdbj.org/pub/pdb/validation_reports/lw/6lw4 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 43883.719 Da / Num. of mol.: 1 / Mutation: C83A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) (bacteria) Gene: lpg2148 / Production host: Escherichia coli (E. coli) / References: UniProt: Q5ZTL3 |
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#2: Protein | Mass: 12646.512 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) (bacteria) Gene: lpg2149 / Production host: Escherichia coli (E. coli) / References: UniProt: Q5ZTL2 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.55 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion / Details: PEG3350, Magnesium chloride hexahydrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.979 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jan 16, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. obs: 13640 / % possible obs: 99.8 % / Redundancy: 18.5 % / Rmerge(I) obs: 0.106 / Net I/σ(I): 31.3 |
Reflection shell | Resolution: 2.7→2.798 Å / Rmerge(I) obs: 0.78 / Num. unique obs: 623 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5SUJ, 5DPO Resolution: 2.7→34.23 Å / SU ML: 0.31 / Cross valid method: THROUGHOUT / σ(F): 1.37 / Phase error: 31.28
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 102.35 Å2 / Biso mean: 40.4164 Å2 / Biso min: 10.23 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.7→34.23 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 5
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