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Open data
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Basic information
Entry | Database: PDB / ID: 6f2r | ||||||
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Title | A heterotetramer of human HspB2 and HspB3 | ||||||
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![]() | CHAPERONE / sHSP / crystallin | ||||||
Function / homology | ![]() structural constituent of eye lens / response to unfolded protein / enzyme activator activity / nuclear speck / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Clark, A.R. / Cole, A.R. / Boelens, W.C. / Keep, N.H. / Slingsby, C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Terminal Regions Confer Plasticity to the Tetrameric Assembly of Human HspB2 and HspB3. Authors: Clark, A.R. / Vree Egberts, W. / Kondrat, F.D.L. / Hilton, G.R. / Ray, N.J. / Cole, A.R. / Carver, J.A. / Benesch, J.L.P. / Keep, N.H. / Boelens, W.C. / Slingsby, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 239.8 KB | Display | ![]() |
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PDB format | ![]() | 168.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 541.3 KB | Display | ![]() |
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Full document | ![]() | 551.5 KB | Display | |
Data in XML | ![]() | 40 KB | Display | |
Data in CIF | ![]() | 57.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
-HspB2,Heat shock protein beta-2,Heat shock protein beta-2,Heat shock protein beta-2,Heat shock ... , 2 types, 6 molecules AEICGK
#1: Protein | Mass: 22896.740 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 22045.689 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Heat shock protein beta- ... , 2 types, 6 molecules DFJTVQ
#3: Protein | Mass: 20258.480 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #4: Protein | Mass: 18183.744 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Unknown peptide from HspB2 or ... , 5 types, 9 molecules MNO123WXY
#5: Protein/peptide | Mass: 869.063 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#6: Protein/peptide | Mass: 613.749 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
#7: Protein/peptide | Mass: 528.644 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
#8: Protein/peptide | Mass: 443.539 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #9: Protein/peptide | Mass: 1039.273 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Details
Sequence details | In the chains QTV and JDF it is not certain if this entity maps to HspB3 or HspB2. Here it is ...In the chains QTV and JDF it is not certain if this entity maps to HspB3 or HspB2. Here it is mapped to the "most likely" protein |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.46 Å3/Da / Density % sol: 72.44 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 7.8 Details: 0.6M SODIUM FORMATE, 0.1 M TRIS HCL pH7.8, 7.8% PGA, 2mM GTP |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 25, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 |
Reflection | Resolution: 3.9→126.46 Å / Num. obs: 147958 / % possible obs: 99.8 % / Redundancy: 3.7 % / Biso Wilson estimate: 123.8 Å2 / Rmerge(I) obs: 0.131 / Net I/σ(I): 6.5 |
Reflection shell | Resolution: 3.9→4.11 Å / Rmerge(I) obs: 1.163 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: ENSEMBLE OF 3Q9P, 2WJ5, 2WJ7 Resolution: 3.9→78 Å / Cor.coef. Fo:Fc: 0.801 / Cor.coef. Fo:Fc free: 0.755 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 1.013 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.873 / SU Rfree Blow DPI: 0.502 / SU Rfree Cruickshank DPI: 0.53
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Displacement parameters | Biso mean: 144.7 Å2
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Refine analyze | Luzzati coordinate error obs: 1.01 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.9→78 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.9→4 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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