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- PDB-3vr4: Crystal structure of Enterococcus hirae V1-ATPase [eV1] -

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Basic information

Entry
Database: PDB / ID: 3vr4
TitleCrystal structure of Enterococcus hirae V1-ATPase [eV1]
Components
  • (V-type sodium ATPase subunit ...) x 3
  • V-type sodium ATPase catalytic subunit A
KeywordsHYDROLASE / V-ATPase / Enterococcus hirae / Rotary motor / P-loop / Na(+)-ATPase / ATP Binding
Function / homology
Function and homology information


Na+-transporting two-sector ATPase / sodium-transporting ATP synthase activity, rotational mechanism / sodium-transporting ATPase activity, rotational mechanism / sodium ion transport / proton motive force-driven plasma membrane ATP synthesis / proton-transporting ATP synthase complex / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism / ATP binding
Similarity search - Function
ATPase, V1 complex, subunit F / Rossmann fold - #12240 / ATPase, V1 complex, subunit F, bacterial/archaeal / Bovine Mitochondrial F1-ATPase, ATP Synthase Beta Chain; Chain D, domain3 / Bovine Mitochondrial F1-atpase; Atp Synthase Beta Chain; Chain D, domain 3 / ATPase, V1 complex, subunit D / ATPase, V1 complex, subunit F / ATPase, V1 complex, subunit F superfamily / ATP synthase subunit D / ATP synthase (F/14-kDa) subunit ...ATPase, V1 complex, subunit F / Rossmann fold - #12240 / ATPase, V1 complex, subunit F, bacterial/archaeal / Bovine Mitochondrial F1-ATPase, ATP Synthase Beta Chain; Chain D, domain3 / Bovine Mitochondrial F1-atpase; Atp Synthase Beta Chain; Chain D, domain 3 / ATPase, V1 complex, subunit D / ATPase, V1 complex, subunit F / ATPase, V1 complex, subunit F superfamily / ATP synthase subunit D / ATP synthase (F/14-kDa) subunit / V-type ATP synthase regulatory subunit B/beta / V-type ATP synthase catalytic alpha chain / ATPsynthase alpha/beta subunit, N-terminal extension / ATPsynthase alpha/beta subunit barrel-sandwich domain / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / : / ATPase, F1/V1 complex, beta/alpha subunit, C-terminal / C-terminal domain of V and A type ATP synthase / ATP synthase subunit alpha, N-terminal domain-like superfamily / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain / ATP synthase alpha/beta family, beta-barrel domain / ATPase, alpha/beta subunit, nucleotide-binding domain, active site / ATP synthase alpha and beta subunits signature. / ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain / ATP synthase alpha/beta family, nucleotide-binding domain / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / P-loop containing nucleotide triphosphate hydrolases / Beta Barrel / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
V-type sodium ATPase subunit D / V-type sodium ATPase subunit G / V-type sodium ATPase catalytic subunit A / V-type sodium ATPase subunit B
Similarity search - Component
Biological speciesEnterococcus hirae (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT, SAD / Resolution: 2.172 Å
AuthorsSaijo, S. / Arai, S. / Suzuki, K. / Mizutani, K. / Kakinuma, Y. / Ishizuka-Katsura, Y. / Ohsawa, N. / Terada, T. / Shirouzu, M. / Yokoyama, S. ...Saijo, S. / Arai, S. / Suzuki, K. / Mizutani, K. / Kakinuma, Y. / Ishizuka-Katsura, Y. / Ohsawa, N. / Terada, T. / Shirouzu, M. / Yokoyama, S. / Iwata, S. / Yamato, I. / Murata, T.
CitationJournal: Nature / Year: 2013
Title: Rotation mechanism of Enterococcus hirae V(1)-ATPase based on asymmetric crystal structures
Authors: Arai, S. / Saijo, S. / Suzuki, K. / Mizutani, K. / Kakinuma, Y. / Ishizuka-Katsura, Y. / Ohsawa, N. / Terada, T. / Shirouzu, M. / Yokoyama, S. / Iwata, S. / Yamato, I. / Murata, T.
History
DepositionApr 3, 2012Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jan 16, 2013Provider: repository / Type: Initial release
Revision 1.1Feb 6, 2013Group: Database references
Revision 1.2Nov 8, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / software / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _software.name / _struct_conn.pdbx_leaving_atom_flag / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Revision 1.3Dec 6, 2023Group: Data collection / Category: chem_comp_atom / chem_comp_bond / Item: _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_2
Revision 1.4Oct 9, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: V-type sodium ATPase catalytic subunit A
B: V-type sodium ATPase catalytic subunit A
C: V-type sodium ATPase catalytic subunit A
D: V-type sodium ATPase subunit B
E: V-type sodium ATPase subunit B
F: V-type sodium ATPase subunit B
G: V-type sodium ATPase subunit D
H: V-type sodium ATPase subunit G
hetero molecules


Theoretical massNumber of molelcules
Total (without water)399,40119
Polymers398,0648
Non-polymers1,33711
Water32,3371795
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area37740 Å2
ΔGint-148 kcal/mol
Surface area117040 Å2
MethodPISA
Unit cell
Length a, b, c (Å)127.980, 128.480, 225.930
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 1 types, 3 molecules ABC

#1: Protein V-type sodium ATPase catalytic subunit A / Na(+)-translocating ATPase subunit A / V-type sodium pump catalytic subunit A


Mass: 67473.273 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (bacteria) / Gene: ntpA / Production host: Cell free synthesis / References: UniProt: Q08636, EC: 3.6.3.15

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V-type sodium ATPase subunit ... , 3 types, 5 molecules DEFGH

#2: Protein V-type sodium ATPase subunit B / Na(+)-translocating ATPase subunit B / V-type sodium pump subunit B


Mass: 52424.312 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (bacteria) / Gene: ntpB / Production host: Cell free synthesis / References: UniProt: Q08637, EC: 3.6.3.15
#3: Protein V-type sodium ATPase subunit D / Na(+)-translocating ATPase subunit D / V-type sodium pump subunit D


Mass: 25587.996 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (bacteria) / Gene: ntpD / Production host: Cell free synthesis / References: UniProt: P43435*PLUS, EC: 3.6.3.15
#4: Protein V-type sodium ATPase subunit G / Na(+)-translocating ATPase subunit G / V-type sodium pump subunit G


Mass: 12783.129 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (bacteria) / Gene: ntpG / Production host: Cell free synthesis / References: UniProt: P43455, EC: 3.6.3.15

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Non-polymers , 4 types, 1806 molecules

#5: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C3H8O3
#6: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#7: Chemical ChemComp-B3P / 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL


Mass: 282.334 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C11H26N2O6 / Comment: pH buffer*YM
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1795 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY
Sequence detailsCURRENTLY THE SEQUENCE OF CHAIN G HAS NOT BEEN SUBMITTED TO THE SEQUENCE DATABASE. RESIDUES (-6)-0 ...CURRENTLY THE SEQUENCE OF CHAIN G HAS NOT BEEN SUBMITTED TO THE SEQUENCE DATABASE. RESIDUES (-6)-0 IN CHAIN G ARE EXPRESSION TAGS.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 2

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Sample preparation

CrystalDensity Matthews: 2.33 Å3/Da / Density % sol: 47.28 %
Crystal growTemperature: 296 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: 29.2% PEG3350, 0.1M NaF, 0.1M Bis-Tris propane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K

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Data collection

Diffraction
IDMean temperature (K)Crystal-ID
11001
21001
Diffraction source
SourceSiteBeamlineIDWavelength (Å)
SYNCHROTRONPhoton Factory AR-NW12A10.97919
SYNCHROTRONPhoton Factory AR-NW12A21
Detector
TypeIDDetectorDate
ADSC QUANTUM 210r1CCDDec 16, 2009
ADSC QUANTUM 210r2CCDDec 16, 2009
Radiation
IDMonochromatorProtocolMonochromatic (M) / Laue (L)Scattering typeWavelength-ID
1Double Crystal Si(111)SINGLE WAVELENGTHMx-ray1
2Double Crystal Si(111)SINGLE WAVELENGTHMx-ray1
Radiation wavelength
IDWavelength (Å)Relative weight
10.979191
211
Reflection twinOperator: k,h,-l / Fraction: 0.036
ReflectionResolution: 2.17→37.12 Å / Num. all: 195452 / Num. obs: 195452 / % possible obs: 99.7 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Redundancy: 7.4 % / Biso Wilson estimate: 29.349 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 16.8
Reflection shellResolution: 2.17→2.23 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.535 / Mean I/σ(I) obs: 3.7 / Num. unique all: 13905 / % possible all: 97.1

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Processing

Software
NameVersionClassification
SERGUIdata collection
PHASERphasing
PHENIX(phenix.refine: dev_934)refinement
XDSdata reduction
XDSdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT, SAD
Starting model: 3VR2, 3AON, 3A5C

3vr2

3aon

3a5c


Resolution: 2.172→34.042 Å / Occupancy max: 1 / Occupancy min: 0.5 / FOM work R set: 0.8539 / Cross valid method: THROUGHOUT / σ(F): 1.32 / Phase error: 22.26 / Stereochemistry target values: TWIN_LSQ_F
RfactorNum. reflection% reflectionSelection details
Rfree0.2108 9799 5.09 %RANDOM
Rwork0.1665 ---
all0.1685 ---
obs0.1685 194627 98.96 %-
Solvent computationShrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 40.996 Å2 / ksol: 0.36 e/Å3
Displacement parametersBiso max: 187.02 Å2 / Biso mean: 32.7787 Å2 / Biso min: 8.13 Å2
Baniso -1Baniso -2Baniso -3
1--0.5525 Å2-0 Å2-0 Å2
2---0.2784 Å20 Å2
3---0.8309 Å2
Refinement stepCycle: LAST / Resolution: 2.172→34.042 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms26189 0 87 1795 28071
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00326903
X-RAY DIFFRACTIONf_angle_d0.73836359
X-RAY DIFFRACTIONf_chiral_restr0.0514168
X-RAY DIFFRACTIONf_plane_restr0.0034728
X-RAY DIFFRACTIONf_dihedral_angle_d12.9710089
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.1731-2.21050.26699430.2303175021844592
2.2105-2.25070.27569460.2263178951884195
2.2507-2.2940.25489430.2226180311897495
2.294-2.34080.25739810.2117178471882895
2.3408-2.39170.25469860.2078178611884795
2.3917-2.44730.25119100.2066180571896795
2.4473-2.50840.2539620.2039179431890595
2.5084-2.57620.24758780.2011180061888495
2.5762-2.6520.23619730.1916179381891195
2.652-2.73750.242210080.1891179571896595
2.7375-2.83520.238210410.1819178941893594
2.8352-2.94860.22628760.1774179981887495
2.9486-3.08270.22699410.1699179631890495
3.0827-3.2450.2219590.1625179491890895
3.245-3.44790.21749410.1543180051894695
3.4479-3.71360.186710040.1397178381884294
3.7136-4.08630.17179890.1287177221871194
4.0863-4.67520.15359280.1164176351856393
4.6752-5.88140.16979050.144174821838792
5.8814-28.05170.20148350.1731169551779090
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.89120.42511.35533.98072.40493.2319-0.08550.13910.1111-0.25170.1706-0.0261-0.20940.0848-0.09340.2314-0.02210.0410.14410.02340.20945.291626.325556.8945
20.38960.0546-0.09761.40510.1820.731-0.02790.0080.071-0.17090.0508-0.1671-0.04690.1608-0.01740.1398-0.01320.010.1784-0.04630.210860.89392.369357.6053
31.7876-1.32331.43252.6541-1.37811.6947-0.2891-0.37740.13630.63390.19010.0902-0.0877-0.28320.03090.28050.02640.01430.2484-0.06130.255651.5823-8.486578.003
40.9957-0.51940.42851.9694-0.70581.01080.0519-0.0734-0.16270.28840.0384-0.12280.41220.1323-0.0390.33660.1162-0.05260.1334-0.05340.21763.8019-30.297280.5299
50.70110.4893-0.51720.7409-0.18580.46480.05060.05110.0607-0.1427-0.02950.0516-0.0837-0.1109-0.03080.21960.0238-0.03380.18710.01560.15031.622811.231553.4007
60.9144-0.06360.07450.5837-0.01711.19150.06460.2348-0.0322-0.15550.02550.2291-0.0225-0.3415-0.08210.1333-0.0226-0.05270.21940.00850.2455-16.9775-2.797363.7099
70.7821-0.2497-0.32790.4475-0.22670.71470.00290.0236-0.1481-0.0902-0.0060.13850.1602-0.05330.01050.1641-0.0107-0.02750.1528-0.02350.166-0.7048-9.205468.4242
81.1287-0.3710.10061.3946-0.3861.10330.0329-0.1616-0.31940.0675-0.02170.10010.2494-0.04250.01770.1002-0.0369-0.01430.01640.01810.11471.9854-19.040290.6489
90.73060.16120.59820.07210.41792.9392-0.03960.05430.23-0.0771-0.0074-0.0813-0.0470.0690.05570.24160.05080.00280.11580.01420.250918.017645.875886.0002
102.1011.03580.26711.57210.22030.4913-0.0715-0.13460.611-0.01750.01920.0903-0.12990.03630.03850.24060.0241-0.03160.132-0.07120.375625.545756.5803108.9934
111.4310.00850.48470.4751-0.33510.61310.0224-0.02770.02160.08320.00440.0364-0.058-0.029-0.03140.14420.00420.0030.1205-0.0570.176626.911233.6796107.2243
121.07830.04350.28960.9197-0.60310.78420.01330.082-0.1008-0.05080.0402-0.0480.1086-0.0353-0.05140.0922-0.00090.00730.1061-0.06610.182635.492724.6114103.4307
132.6545-0.4648-0.15142.8902-0.99722.23760.1711-0.312-0.23770.5135-0.03010.160.28350.0643-0.08770.3245-0.0426-0.05330.15990.00880.213136.74235.1405124.9289
144.371-3.2967-1.66582.62271.50311.1283-0.1977-0.13430.06290.12590.1271-0.0062-0.11220.02940.07040.29690.0020.03040.17480.04510.267640.235944.349173.9856
150.61680.0105-0.62190.9356-0.80281.35950.06390.04420.0130.0343-0.042-0.2242-0.15830.2608-0.01040.1473-0.0585-0.05840.2543-0.07170.272663.869126.016886.519
160.7814-0.35350.05881.9001-0.66681.75460.031-0.03930.06660.30540.0215-0.2793-0.23980.342-0.0570.2038-0.0165-0.05550.2111-0.07390.25257.427126.408197.4599
170.3096-0.10750.0360.6764-0.33131.1760.01880.0322-0.0562-0.02130.0508-0.13890.10270.1539-0.06290.1163-0.0029-0.03310.1636-0.07590.241954.470314.36888.8359
181.96290.01230.06850.4755-0.77131.68650.1066-0.081-0.57310.1746-0.0882-0.51170.34020.27830.03480.30470.0868-0.17010.2601-0.040.49663.9146-2.4053107.9464
194.67942.76630.80992.35860.17010.3022-0.06510.16160.0466-0.24040.11130.01660.02050.0156-0.04810.27420.02940.03780.17460.01950.151129.488212.41845.8493
201.62990.47240.55881.472-0.21841.6933-0.00510.183-0.1233-0.2479-0.0335-0.10660.15120.04720.04840.1710.02440.00360.1285-0.04370.152624.8038-20.13953.2266
211.70320.050.14461.0209-0.33720.6442-0.0310.08890.0535-0.06530.021-0.08640.01310.05220.0080.0926-0.0105-0.00780.0938-0.01860.097926.8734-12.251158.8326
220.90010.34410.17710.7929-0.14290.44840.2662-0.5848-0.46770.3186-0.1748-0.35860.52160.0013-0.03780.351-0.1214-0.19510.18920.16640.286123.0926-37.127175.9523
231.76270.60271.43762.71992.03473.9304-0.03280.016-0.01340.05490.02560.1580.013-0.08010.00690.21330.05060.04650.13530.05460.1982-0.946931.022970.6091
241.22810.1585-1.35725.87060.771.93290.04350.2724-0.0573-0.41020.0090.6206-0.0419-0.4124-0.06430.17570.0577-0.05250.24840.00760.2491-14.885124.692690.1758
250.2024-0.08280.10560.70070.09760.6992-0.0101-0.06320.02650.01350.02130.04930.0024-0.1276-0.01210.12340.0060.01690.1444-0.00440.1499-2.238617.139498.5215
262.00020.2057-1.40872.08750.05083.8759-0.16350.0073-0.1309-0.19730.0816-0.2040.03280.21790.01520.1219-0.0208-0.01550.1049-0.03830.208110.146124.033192.137
271.1923-0.3432-0.42590.8289-0.92381.91260.0071-0.0209-0.05240.0591-0.0039-0.06360.05040.0278-0.00430.1169-0.0171-0.01910.1299-0.00840.17869.79339.9829104.4706
282.1538-0.19220.90581.32960.30862.50840.0197-0.2233-0.34430.33560.0522-0.10540.46560.2085-0.04680.28490.03430.00040.18970.08790.183313.2535-0.7931119.7914
290.8786-0.18890.2123.4083-2.12152.63060.0555-0.2206-0.06840.1524-0.06910.0990.05460.16730.02310.16230.0001-0.02650.2145-0.0110.149635.2215-32.294115.7607
300.3401-0.550.26234.0791-3.30912.99320.0182-0.30170.0834-0.01660.13990.22660.04510.0614-0.05350.035-0.0573-0.0844-0.0406-0.00710.052933.5912-20.0024107.395
314.88430.38691.04914.63761.33125.2990.3353-0.9256-0.08160.162-0.09240.1094-0.7435-0.426-0.2150.43210.11330.16270.4750.13970.399328.1621-38.4723134.6169
322.64121.70912.33082.17982.13852.4270.5391-0.874-0.49560.7118-0.14070.74140.6499-1.2864-0.63360.3875-0.07660.04680.72880.31310.538227.4375-49.3926137.1967
335.61620.52360.89552.63320.10842.49620.6214-0.2820.25040.4303-0.35620.64110.3295-0.9137-0.29830.5246-0.01940.09520.73640.18080.668821.1806-43.0874135.2277
341.86342.61041.12153.8031.70581.54880.1620.17610.31580.1146-0.19340.7385-0.1911-0.77960.07940.38190.19470.27250.7470.19780.809717.6732-35.1955130.6311
354.39740.27920.94824.0974-0.62672.83680.1399-0.15310.2288-0.01210.07221.0823-0.3461-0.9612-0.11830.24140.10590.0680.44860.15850.471123.2729-38.4179126.1476
362.27580.309-1.0910.3590.1760.85540.1199-0.03990.6688-0.38070.15440.0391-0.28680.3189-0.00450.4570.175-0.01180.41190.07050.437828.6524-29.541124.4173
372.5411-1.3481-0.08021.2384-0.75364.71550.176-0.3008-0.48250.00930.1260.89750.8182-1.0434-0.26290.3422-0.1101-0.13450.49240.20440.512826.1365-48.0935123.7805
383.81765.0495-4.53976.6798-6.00495.3975-0.11610.8855-0.0352-1.03930.92570.94930.9599-1.2692-0.62750.4129-0.06-0.20490.36350.08070.405630.2013-40.2619110.651
391.1079-0.60220.76992.47671.56412.3640.06920.97820.1412-2.54070.14320.42430.9049-0.0717-0.24980.7302-0.0315-0.01770.42990.19250.540737.1992-40.4914104.5676
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain A and (resseq 0:63)A0 - 63
2X-RAY DIFFRACTION2chain A and (resseq 64:390)A64 - 390
3X-RAY DIFFRACTION3chain A and (resseq 391:426)A391 - 426
4X-RAY DIFFRACTION4chain A and (resseq 427:587)A427 - 587
5X-RAY DIFFRACTION5chain B and (resseq -6:90)B-6 - 90
6X-RAY DIFFRACTION6chain B and (resseq 91:226)B91 - 226
7X-RAY DIFFRACTION7chain B and (resseq 227:344)B227 - 344
8X-RAY DIFFRACTION8chain B and (resseq 345:586)B345 - 586
9X-RAY DIFFRACTION9chain C and (resseq 1:90)C1 - 90
10X-RAY DIFFRACTION10chain C and (resseq 91:185)C91 - 185
11X-RAY DIFFRACTION11chain C and (resseq 186:344)C186 - 344
12X-RAY DIFFRACTION12chain C and (resseq 345:452)C345 - 452
13X-RAY DIFFRACTION13chain C and (resseq 453:586)C453 - 586
14X-RAY DIFFRACTION14chain D and (resseq 4:77)D4 - 77
15X-RAY DIFFRACTION15chain D and (resseq 78:147)D78 - 147
16X-RAY DIFFRACTION16chain D and (resseq 148:217)D148 - 217
17X-RAY DIFFRACTION17chain D and (resseq 218:389)D218 - 389
18X-RAY DIFFRACTION18chain D and (resseq 390:455)D390 - 455
19X-RAY DIFFRACTION19chain E and (resseq 4:77)E4 - 77
20X-RAY DIFFRACTION20chain E and (resseq 78:187)E78 - 187
21X-RAY DIFFRACTION21chain E and (resseq 188:330)E188 - 330
22X-RAY DIFFRACTION22chain E and (resseq 331:455)E331 - 455
23X-RAY DIFFRACTION23chain F and (resseq 1:77)F1 - 77
24X-RAY DIFFRACTION24chain F and (resseq 78:106)F78 - 106
25X-RAY DIFFRACTION25chain F and (resseq 107:263)F107 - 263
26X-RAY DIFFRACTION26chain F and (resseq 264:296)F264 - 296
27X-RAY DIFFRACTION27chain F and (resseq 297:364)F297 - 364
28X-RAY DIFFRACTION28chain F and (resseq 365:455)F365 - 455
29X-RAY DIFFRACTION29chain G and (resseq 6:89)G6 - 89
30X-RAY DIFFRACTION30chain G and (resseq 90:206)G90 - 206
31X-RAY DIFFRACTION31chain H and (resseq 2:10)H2 - 10
32X-RAY DIFFRACTION32chain H and (resseq 11:20)H11 - 20
33X-RAY DIFFRACTION33chain H and (resseq 21:29)H21 - 29
34X-RAY DIFFRACTION34chain H and (resseq 30:42)H30 - 42
35X-RAY DIFFRACTION35chain H and (resseq 43:64)H43 - 64
36X-RAY DIFFRACTION36chain H and (resseq 65:71)H65 - 71
37X-RAY DIFFRACTION37chain H and (resseq 72:84)H72 - 84
38X-RAY DIFFRACTION38chain H and (resseq 85:97)H85 - 97
39X-RAY DIFFRACTION39chain H and (resseq 98:103)H98 - 103

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