+Open data
-Basic information
Entry | Database: PDB / ID: 3uit | ||||||
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Title | Overall structure of Patj/Pals1/Mals complex | ||||||
Components | InaD-like protein, MAGUK p55 subfamily member 5, Protein lin-7 homolog B | ||||||
Keywords | CELL ADHESION / L27 domain / CELL POLARIZATION | ||||||
Function / homology | Function and homology information RHO GTPases Activate Rhotekin and Rhophilins / protein localization to myelin sheath abaxonal region / protein localization to basolateral plasma membrane / SARS-CoV-1 targets PDZ proteins in cell-cell junction / establishment or maintenance of polarity of embryonic epithelium / myelin assembly / L27 domain binding / MPP7-DLG1-LIN7 complex / morphogenesis of an epithelial sheet / Tight junction interactions ...RHO GTPases Activate Rhotekin and Rhophilins / protein localization to myelin sheath abaxonal region / protein localization to basolateral plasma membrane / SARS-CoV-1 targets PDZ proteins in cell-cell junction / establishment or maintenance of polarity of embryonic epithelium / myelin assembly / L27 domain binding / MPP7-DLG1-LIN7 complex / morphogenesis of an epithelial sheet / Tight junction interactions / SARS-CoV-2 targets PDZ proteins in cell-cell junction / maintenance of epithelial cell apical/basal polarity / Dopamine Neurotransmitter Release Cycle / lateral loop / myelin sheath adaxonal region / regulation of transforming growth factor beta receptor signaling pathway / Schmidt-Lanterman incisure / neurotransmitter secretion / peripheral nervous system myelin maintenance / establishment or maintenance of epithelial cell apical/basal polarity / cell-cell junction assembly / apical junction complex / generation of neurons / central nervous system neuron development / exocytosis / centriolar satellite / bicellular tight junction / endoplasmic reticulum-Golgi intermediate compartment membrane / protein localization to plasma membrane / PDZ domain binding / adherens junction / postsynaptic density membrane / cerebral cortex development / cell-cell junction / protein transport / presynapse / apical part of cell / cell junction / gene expression / basolateral plasma membrane / perikaryon / apical plasma membrane / protein domain specific binding / axon / centrosome / synapse / perinuclear region of cytoplasm / Golgi apparatus / protein-containing complex / extracellular exosome / ATP binding / identical protein binding / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) Rattus norvegicus (Norway rat) Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.05 Å | ||||||
Authors | Zhang, J. / Yang, X. / Long, J. / Shen, Y. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2012 Title: Structure of an L27 domain heterotrimer from cell polarity complex Patj/Pals1/Mals2 reveals mutually independent L27 domain assembly mode Authors: Zhang, J. / Yang, X. / Wang, Z. / Zhou, H. / Xie, X. / Shen, Y. / Long, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3uit.cif.gz | 448.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3uit.ent.gz | 370.8 KB | Display | PDB format |
PDBx/mmJSON format | 3uit.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3uit_validation.pdf.gz | 483.6 KB | Display | wwPDB validaton report |
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Full document | 3uit_full_validation.pdf.gz | 511.1 KB | Display | |
Data in XML | 3uit_validation.xml.gz | 49.6 KB | Display | |
Data in CIF | 3uit_validation.cif.gz | 71 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ui/3uit ftp://data.pdbj.org/pub/pdb/validation_reports/ui/3uit | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 29856.209 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: Chimera of L27 domains of InaD-like protein (UNP residues 1-68) from Mouse, LINKER (LEVLFQGP), MAGUK p55 subfamily member 5 (UNP residues 119-232) from Human, LINKER (GGGLEVFQGP), Protein ...Details: Chimera of L27 domains of InaD-like protein (UNP residues 1-68) from Mouse, LINKER (LEVLFQGP), MAGUK p55 subfamily member 5 (UNP residues 119-232) from Human, LINKER (GGGLEVFQGP), Protein lin-7 homolog B (UNP residues 3-66) from Rat Source: (gene. exp.) Mus musculus (house mouse), (gene. exp.) Rattus norvegicus (Norway rat), (gene. exp.) Homo sapiens (human) Production host: Escherichia coli (E. coli) References: UniProt: Q63ZW7, UniProt: Q8N3R9, UniProt: Q9Z252, UniProt: F1MAD2*PLUS #2: Chemical | ChemComp-ACT / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.33 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 2.8M sodium acetate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 | ||||||||||||||||||
Reflection | Resolution: 2.05→50 Å / Num. obs: 79261 / % possible obs: 87.7 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 20.2 % / Biso Wilson estimate: 29.21 Å2 / Rmerge(I) obs: 0.063 / Rsym value: 0.081 / Net I/σ(I): 36.2 | ||||||||||||||||||
Reflection shell | Resolution: 2.05→2.12 Å / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.05→38.863 Å / Occupancy max: 1 / Occupancy min: 1 / FOM work R set: 0.8479 / SU ML: 0.22 / σ(F): 2 / Phase error: 21.86 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 49.246 Å2 / ksol: 0.343 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 224.64 Å2 / Biso mean: 41.2893 Å2 / Biso min: 13.37 Å2
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Refinement step | Cycle: LAST / Resolution: 2.05→38.863 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 10
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Refinement TLS params. | Method: refined / Origin x: -43.3383 Å / Origin y: 27.7285 Å / Origin z: 34.3325 Å
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Refinement TLS group |
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