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- PDB-1r4c: N-Truncated Human Cystatin C; Dimeric Form With 3D Domain Swapping -
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Open data
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Basic information
Entry | Database: PDB / ID: 1r4c | ||||||
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Title | N-Truncated Human Cystatin C; Dimeric Form With 3D Domain Swapping | ||||||
![]() | Cystatin C | ||||||
![]() | HYDROLASE INHIBITOR / HUMAN CYSTATIN C / N-TRUNCATION / 3D DOMAIN SWAPPING / AMYLOID FORMATION / INHIBITOR OF C1 AND C13 CYSTEINE PROTEASES / AMYLOID ANGIOPATHY AND CEREBRAL HEMORRHAGE | ||||||
Function / homology | ![]() negative regulation of collagen catabolic process / negative regulation of elastin catabolic process / negative regulation of blood vessel remodeling / negative regulation of peptidase activity / peptidase inhibitor activity / negative regulation of extracellular matrix disassembly / regulation of tissue remodeling / cysteine-type endopeptidase inhibitor activity / endopeptidase inhibitor activity / supramolecular fiber organization ...negative regulation of collagen catabolic process / negative regulation of elastin catabolic process / negative regulation of blood vessel remodeling / negative regulation of peptidase activity / peptidase inhibitor activity / negative regulation of extracellular matrix disassembly / regulation of tissue remodeling / cysteine-type endopeptidase inhibitor activity / endopeptidase inhibitor activity / supramolecular fiber organization / negative regulation of proteolysis / Post-translational protein phosphorylation / defense response / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / tertiary granule lumen / amyloid-beta binding / protease binding / vesicle / ficolin-1-rich granule lumen / Amyloid fiber formation / endoplasmic reticulum lumen / Neutrophil degranulation / Golgi apparatus / endoplasmic reticulum / extracellular space / extracellular exosome / extracellular region / identical protein binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Janowski, R. / Abrahamson, M. / Grubb, A. / Jaskolski, M. | ||||||
![]() | ![]() Title: Domain swapping in N-truncated human cystatin C. Authors: Janowski, R. / Abrahamson, M. / Grubb, A. / Jaskolski, M. #1: ![]() Title: Human cystatin C, an amyloidogenic protein, dimerizes through three-dimensional domain swapping Authors: Janowski, R. / Kozak, M. / Jankowska, E. / Grzonka, Z. / Grubb, A. / Abrahamson, M. / Jaskolski, M. #2: ![]() Title: Expression of a selenomethionyl derivative and preliminary crystallographic studies of human cystatin C Authors: Kozak, M. / Jankowska, E. / Janowski, R. / Grzonka, Z. / Grubb, A. / Alvarez Fernandez, M. / Abrahamson, M. / Jaskolski, M. #3: ![]() Title: NMR structural studies of human cystatin C dimers and monomers Authors: Ekiel, I. / Abrahamson, M. / Fulton, D.B. / Lindahl, P. / Storer, A.C. / Levadoux, W. / Lafrance, M. / Labelle, S. / Pomerleau, Y. / Groleau, D. / LeSauteur, L. / Gehring, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 182.6 KB | Display | ![]() |
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PDB format | ![]() | 148.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 486.9 KB | Display | ![]() |
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Full document | ![]() | 518.2 KB | Display | |
Data in XML | ![]() | 36.5 KB | Display | |
Data in CIF | ![]() | 50.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1g96S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | THE EIGHT POLYPEPTIDE CHAINS ARE ASSEMBLED INTO 3D DOMAIN SWAPPED DIMERS IN THE FOLLOWING WAY: AB, CB, EF, GH |
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Components
#1: Protein | Mass: 12343.925 Da / Num. of mol.: 8 / Fragment: human cystatin C without 10 N-terminal residues Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.28 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 8.1 Details: 0.4M (NH4)H2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 292K, pH 8.1 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Mar 24, 1999 |
Radiation | Monochromator: SINGLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.104 Å / Relative weight: 1 |
Reflection | Resolution: 2.18→25 Å / Num. obs: 52404 / % possible obs: 99.2 % / Observed criterion σ(I): 0 / Redundancy: 9.3 % / Biso Wilson estimate: 36.9 Å2 / Rmerge(I) obs: 0.041 / Net I/σ(I): 33.6 |
Reflection shell | Resolution: 2.18→2.26 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.126 / Mean I/σ(I) obs: 10.9 / % possible all: 98.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: HUMAN CYSTATIN C DIMER WITH SWAPPED DOMAINS (PDB ENTRY 1G96) Resolution: 2.18→10 Å / SU B: 8.514 / SU ML: 0.226 / TLS residual ADP flag: LIKELY RESIDUAL / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.284 / ESU R Free: 0.219 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: THE REFINEMENT INCLUDED TLS PARAMETERS, HYDROGENS HAVE BEEN ADDED IN THE RIGID POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.75 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.18→10 Å
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Refine LS restraints |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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