+Open data
-Basic information
Entry | Database: PDB / ID: 1a67 | ||||||
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Title | CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES | ||||||
Components | CYSTATIN | ||||||
Keywords | PROTEINASE INHIBITOR / THIOL PROTEINASE / STEFFINS / KININOGENS | ||||||
Function / homology | Function and homology information cysteine-type endopeptidase inhibitor activity / vesicle / extracellular space / cytoplasm Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
Authors | Dieckmann, T. / Mitschang, L. / Hofmann, M. / Kos, J. / Turk, V. / Auerswald, E.A. / Jaenicke, R. / Oschkinat, H. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1993 Title: The structures of native phosphorylated chicken cystatin and of a recombinant unphosphorylated variant in solution. Authors: Dieckmann, T. / Mitschang, L. / Hofmann, M. / Kos, J. / Turk, V. / Auerswald, E.A. / Jaenicke, R. / Oschkinat, H. #1: Journal: J.Mol.Biol. / Year: 1993 Title: Conformational Variability of Chicken Cystatin. Comparison of Structures Determined by X-Ray Diffraction and NMR Spectroscopy Authors: Engh, R.A. / Dieckmann, T. / Bode, W. / Auerswald, E.A. / Turk, V. / Huber, R. / Oschkinat, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a67.cif.gz | 529.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a67.ent.gz | 457.1 KB | Display | PDB format |
PDBx/mmJSON format | 1a67.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a6/1a67 ftp://data.pdbj.org/pub/pdb/validation_reports/a6/1a67 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12204.868 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: PHOSPHORYLATED / Source: (natural) Gallus gallus (chicken) / Cell: EGG / Cellular location: CYTOPLASM (WHITE) / Organ: EGG / References: UniProt: P01038 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 5.5 / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: DISTANCE GEOMETRY, SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST OVERALL ENERGY AND RESTRAINT VIOLATIONS Conformers calculated total number: 50 / Conformers submitted total number: 16 |