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- PDB-1cew: THE 2.0 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF CHICKEN EGG WHITE CY... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1cew | ||||||
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Title | THE 2.0 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF CHICKEN EGG WHITE CYSTATIN AND ITS POSSIBLE MODE OF INTERACTION WITH CYSTEINE PROTEINASES | ||||||
![]() | CYSTATIN | ||||||
![]() | PROTEINASE INHIBITOR(CYSTEINE) | ||||||
Function / homology | ![]() cysteine-type endopeptidase inhibitor activity / vesicle / extracellular space / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Bode, W. / Musil, D. / Huber, R. | ||||||
![]() | ![]() Title: The 2.0 A X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases. Authors: Bode, W. / Engh, R. / Musil, D. / Thiele, U. / Huber, R. / Karshikov, A. / Brzin, J. / Kos, J. / Turk, V. #1: ![]() Title: Conformational Variability of Chicken Cystatin: Comparison of Structures Determined by X-Ray Diffraction and NMR-Spectroscopy Authors: Engh, R.A. / Dieckmann, T. / Bode, W. / Auerswald, E.A. / Turk, V. / Huber, R. / Oschkinat, H. #2: ![]() Title: The Structures of Native Phosphorylated Chicken Cystatin and of a Recombinant Unphosphorylated Variant in Solution Authors: Dieckmann, T. / Mitschang, L. / Hofmann, M. / Kos, J. / Turk, V. / Auerswald, E.A. / Jaenicke, R. / Oschkinat, H. #3: ![]() Title: The Cystatins: Protein Inhibitors of Cysteine Proteinases Authors: Turk, V. / Bode, W. #4: ![]() Title: Mechanism of Interaction of Cysteine Proteinases and Their Protein Inhibitors as Compared to the Serine Proteinase-Inhibitor Interaction Authors: Bode, W. / Engh, R. / Musil, D. / Laber, B. / Stubbs, M. / Huber, R. / Turk, V. #5: ![]() Title: Mechanism of Inhibition of Papain by Chicken Egg White Cystatin: Inhibition Constants of N-Terminally Truncated Forms and Cyanogen Bromide Fragments of the Inhibitor Authors: Machleidt, W. / Thiele, U. / Laber, B. / Assfalg-Machleidt, I. / Esterl, A. / Wiegand, G. / Kos, J. / Turk, V. / Bode, W. #6: ![]() Title: The Cysteine Proteinase Inhibitor Chicken Cystatin is a Phophoprotein Authors: Laber, B. / Krieglstein, K. / Henschen, A. / Kos, J. / Turk, V. / Huber, R. / Bode, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 37.2 KB | Display | ![]() |
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PDB format | ![]() | 25.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 367.4 KB | Display | ![]() |
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Full document | ![]() | 374.7 KB | Display | |
Data in XML | ![]() | 4.8 KB | Display | |
Data in CIF | ![]() | 7.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: GLU I 88 - MET I 89 OMEGA =121.89 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION |
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Components
#1: Protein | Mass: 12204.868 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.17 % | ||||||||||||||||||
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Crystal grow | *PLUS pH: 8.2 / Method: unknown | ||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2 Å / Num. obs: 6715 / % possible obs: 75 % / Rmerge(I) obs: 0.105 |
Reflection shell | *PLUS Highest resolution: 2 Å / Lowest resolution: 2.2 Å / % possible obs: 30 % |
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Processing
Software | Name: EREF / Classification: refinement | ||||||||||||
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Refinement | Rfactor Rwork: 0.198 / Highest resolution: 2 Å | ||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Software | *PLUS Name: EREF / Classification: refinement | ||||||||||||
Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 6 Å / Num. reflection obs: 6181 / Rfactor obs: 0.198 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS Biso mean: 20 Å2 | ||||||||||||
Refine LS restraints | *PLUS
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