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Open data
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Basic information
Entry | Database: PDB / ID: 3eot | ||||||
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Title | Crystal structure of LAC031, an engineered anti-VLA1 Fab | ||||||
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![]() | IMMUNE SYSTEM / antibody / Fab / VLA-1 / domain swap / proten engineering | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Boriack-Sjodin, P.A. / Clark, L.A. | ||||||
![]() | ![]() Title: An antibody loop replacement design feasibility study and a loop-swapped dimer structure. Authors: Clark, L.A. / Boriack-Sjodin, P.A. / Day, E. / Eldredge, J. / Fitch, C. / Jarpe, M. / Miller, S. / Li, Y. / Simon, K. / van Vlijmen, H.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 93.2 KB | Display | ![]() |
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PDB format | ![]() | 74.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436.7 KB | Display | ![]() |
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Full document | ![]() | 445.1 KB | Display | |
Data in XML | ![]() | 19.2 KB | Display | |
Data in CIF | ![]() | 27.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 24224.074 Da / Num. of mol.: 1 / Mutation: W92I Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Antibody | Mass: 23514.824 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 49.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 16-20% Peg 3350, 100 mM Na Cacodylate, 200 mM MgCl2 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 1, 2006 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.9→50 Å / Num. obs: 36217 / % possible obs: 96.3 % / Redundancy: 6.7 % / Rmerge(I) obs: 0.06 / Χ2: 1.169 / Net I/σ(I): 35.365 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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Processing
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Refinement | Resolution: 1.9→35 Å / Occupancy max: 1 / Occupancy min: 1 / FOM work R set: 0.792 / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso max: 66.08 Å2 / Biso mean: 34.008 Å2 / Biso min: 14.28 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.9→35 Å
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Refine LS restraints |
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Xplor file |
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