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- PDB-4fqq: Crystal Structure of Germline Antibody PGT121-GL Fab -

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Open data


ID or keywords:

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Basic information

Entry
Database: PDB / ID: 4fqq
TitleCrystal Structure of Germline Antibody PGT121-GL Fab
Components
  • Fab heavy chainFragment antigen-binding
  • Fab light chainFragment antigen-binding
KeywordsIMMUNE SYSTEM / IG FOLD / ANTI HIV / ANTIBODY
Function / homology
Function and homology information


Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain ...Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
IGH@ protein / IGL@ protein
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.42 Å
AuthorsScharf, L. / Bjorkman, P.J.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2012
Title: Complex-type N-glycan recognition by potent broadly neutralizing HIV antibodies.
Authors: Mouquet, H. / Scharf, L. / Euler, Z. / Liu, Y. / Eden, C. / Scheid, J.F. / Halper-Stromberg, A. / Gnanapragasam, P.N. / Spencer, D.I. / Seaman, M.S. / Schuitemaker, H. / Feizi, T. / ...Authors: Mouquet, H. / Scharf, L. / Euler, Z. / Liu, Y. / Eden, C. / Scheid, J.F. / Halper-Stromberg, A. / Gnanapragasam, P.N. / Spencer, D.I. / Seaman, M.S. / Schuitemaker, H. / Feizi, T. / Nussenzweig, M.C. / Bjorkman, P.J.
History
DepositionJun 25, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 14, 2012Provider: repository / Type: Initial release
Revision 1.1Dec 12, 2012Group: Database references
Revision 1.2May 19, 2021Group: Advisory / Derived calculations / Source and taxonomy
Category: entity_src_gen / pdbx_unobs_or_zero_occ_atoms / struct_site
Item: _entity_src_gen.gene_src_common_name / _entity_src_gen.host_org_common_name ..._entity_src_gen.gene_src_common_name / _entity_src_gen.host_org_common_name / _entity_src_gen.pdbx_host_org_cell_line / _entity_src_gen.pdbx_host_org_strain / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
L: Fab light chain
H: Fab heavy chain
A: Fab light chain
B: Fab heavy chain
C: Fab light chain
D: Fab heavy chain
E: Fab light chain
F: Fab heavy chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)197,59710
Polymers197,5518
Non-polymers462
Water8,827490
1
A: Fab light chain
B: Fab heavy chain


Theoretical massNumber of molelcules
Total (without water)49,3882
Polymers49,3882
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3470 Å2
ΔGint-26 kcal/mol
Surface area20190 Å2
MethodPISA
2
C: Fab light chain
D: Fab heavy chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,4113
Polymers49,3882
Non-polymers231
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3460 Å2
ΔGint-23 kcal/mol
Surface area20190 Å2
MethodPISA
3
E: Fab light chain
F: Fab heavy chain


Theoretical massNumber of molelcules
Total (without water)49,3882
Polymers49,3882
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3500 Å2
ΔGint-26 kcal/mol
Surface area19950 Å2
MethodPISA
4
L: Fab light chain
H: Fab heavy chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,4113
Polymers49,3882
Non-polymers231
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4100 Å2
ΔGint-49 kcal/mol
Surface area20130 Å2
MethodPISA
Unit cell
Length a, b, c (Å)54.934, 344.743, 55.230
Angle α, β, γ (deg.)90.00, 91.95, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Antibody
Fab light chain / Fragment antigen-binding


Mass: 22915.217 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293-6E / Production host: Homo sapiens (human) / References: UniProt: Q8N355*PLUS
#2: Antibody
Fab heavy chain / Fragment antigen-binding


Mass: 26472.586 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293-6E / Production host: homo sapiens (human) / References: UniProt: Q6GMX6*PLUS
#3: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Na
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 490 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.65 Å3/Da / Density % sol: 53.51 %
Crystal growTemperature: 298 K / Method: vapor diffusion / pH: 7
Details: PEG 3350, sodium malonate, MnCl2, pH 7.0, vapor diffusion, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 1.033 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 9, 2012
RadiationMonochromator: LIQUID NITROGEN-COOLED DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.033 Å / Relative weight: 1
ReflectionResolution: 2.419→38.305 Å / Num. all: 74318 / Num. obs: 74318 / % possible obs: 95.5 % / Observed criterion σ(I): -3 / Redundancy: 3.1 % / Rsym value: 0.072 / Net I/σ(I): 11
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsRsym valueDiffraction-ID% possible all
2.42-2.552.60.4821.60.482180.1
2.55-2.73.20.3512.10.351199
2.7-2.893.10.2243.20.224198.7
2.89-3.123.10.14450.144198.1
3.12-3.423.10.0967.40.096198.4
3.42-3.8230.06510.80.065197.5
3.82-4.423.20.04814.50.048198.3
4.42-5.413.10.03917.40.039197.7
5.41-7.653.10.04116.40.041197.7
7.65-38.3053.10.028220.028196.2

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Processing

Software
NameVersionClassificationNB
SCALA3.3.16data scaling
PHENIX1.8_1069refinement
PDB_EXTRACT3.11data extraction
XDSdata scaling
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.42→37.44 Å / Occupancy max: 1 / Occupancy min: 0 / SU ML: 0.33 / σ(F): 1.36 / Phase error: 28.02 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.237 1992 2.68 %
Rwork0.194 --
obs0.195 74237 95.5 %
all-77751 -
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 48 Å2
Refinement stepCycle: LAST / Resolution: 2.42→37.44 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13261 0 2 490 13753
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00513647
X-RAY DIFFRACTIONf_angle_d0.95118637
X-RAY DIFFRACTIONf_dihedral_angle_d12.9164802
X-RAY DIFFRACTIONf_chiral_restr0.0672098
X-RAY DIFFRACTIONf_plane_restr0.0062363
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.4188-2.47930.3562950.29663474X-RAY DIFFRACTION64
2.4793-2.54630.35581370.28425131X-RAY DIFFRACTION96
2.5463-2.62130.32671450.26535382X-RAY DIFFRACTION99
2.6213-2.70580.31391400.25795290X-RAY DIFFRACTION99
2.7058-2.80250.29151570.24975282X-RAY DIFFRACTION99
2.8025-2.91470.30321420.24465418X-RAY DIFFRACTION98
2.9147-3.04730.31071470.24245306X-RAY DIFFRACTION98
3.0473-3.20790.31961400.22515278X-RAY DIFFRACTION99
3.2079-3.40870.27991570.21045259X-RAY DIFFRACTION98
3.4087-3.67170.24671510.19375287X-RAY DIFFRACTION97
3.6717-4.04080.21411380.17055285X-RAY DIFFRACTION99
4.0408-4.62460.17521470.13965320X-RAY DIFFRACTION98
4.6246-5.8230.14721490.14375256X-RAY DIFFRACTION98
5.823-37.44820.20281470.17695277X-RAY DIFFRACTION97
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
13.7219-0.85790.16023.6514-0.51820.92340.02720.2354-0.1601-0.0694-0.09090.27820.3674-0.15310.06810.4414-0.0205-0.02410.3196-0.03880.165854.0945-19.187312.8731
25.8233.1548-3.39223.0459-2.73954.4353-0.16760.1584-0.1084-0.38870.1397-0.2177-0.1766-0.0441-0.08340.32820.0466-0.02650.2757-0.040.263855.381620.388916.1739
31.88850.482-1.00157.1366-0.222.56540.17910.07750.2406-0.6019-0.15050.3093-0.2575-0.299-0.04720.25850.0862-0.07560.3402-0.050.255448.305519.978315.3477
43.3435-0.47210.87673.65690.11691.7495-0.01410.01960.2043-0.1252-0.0651-0.3667-0.11480.130.06010.44630.03790.02790.28230.02450.189181.6909-29.3479-12.2902
55.0746-2.05213.37125.8862-3.58725.4259-0.00120.41790.1221-0.48030.041-0.3436-0.11480.2184-0.02920.47110.05790.14570.316-0.02020.284280.2563-31.7774-16.5169
60.7352-0.4585-0.13384.0784-1.16921.50880.01090.0324-0.2121-0.5650.0263-0.23260.36120.0229-0.03650.45570.1020.06310.32120.02820.273483.2362-60.8983-11.523
77.0704-1.3941-0.61044.82842.03254.10360.24670.4716-1.2161-0.6664-0.1454-0.6780.3225-0.3064-0.09511.00850.14980.1820.48490.04490.712890.8991-75.0669-17.7286
82.40640.41510.76161.7422-0.63321.13380.06880.2228-0.4831-0.6461-0.1326-0.00170.64440.21730.06360.78720.1219-0.0250.3432-0.01670.442982.9944-68.8008-12.9874
97.60135.20961.79092.14322.87345.28270.29450.1877-1.1264-0.5846-0.222-1.41990.90440.4949-0.09980.57760.17410.08140.34420.0650.563389.9508-71.0624-6.6093
102.0628-1.0288-0.03173.37530.21392.3845-0.1353-0.44670.23420.29080.04590.0192-0.05050.04570.12110.20580.0211-0.01770.4071-0.11290.536193.672353.4194-2.3776
116.91851.59070.14383.7974-1.7080.8815-0.221-0.42850.52690.67180.6481-0.0645-0.20030.7976-0.46360.33490.04790.02660.5266-0.26820.644292.090758.7791.9333
123.26971.1339-0.27583.9385-0.95473.1838-0.2856-0.1836-0.00050.39320.50140.30740.20350.019-0.27020.33930.07470.06010.4058-0.06570.574190.784847.4592-2.0486
138.10153.4382-0.77819.8694-0.99926.1088-0.2239-0.74990.9097-0.0477-0.6734-0.9709-0.641.66720.85630.36470.0127-0.19530.6992-0.00860.7021108.500757.5719-5.6747
142.33953.08841.33651.9972-2.52123.8982-1.4646-0.2854-0.66660.9365-0.03090.0906-0.4424-0.88750.76730.7771-0.06560.63930.4897-0.05740.700385.988534.94854.3956
152.7483-1.4235-1.51035.9492.49314.8623-0.0727-0.11760.01180.745-0.18090.46590.0921-0.22340.21970.3832-0.02690.12710.3525-0.0110.304393.795314.1209-8.3773
163.62930.2613-2.11384.4913-0.11953.46560.1298-0.06130.08060.4139-0.3989-0.01910.4288-0.03910.16420.4676-0.06910.03770.4275-0.01870.259394.89319.5454-2.2675
174.76780.3438-0.76272.6463-2.51054.5557-0.1871-0.244-0.87550.9337-0.29160.25691.12780.27740.41850.66720.08310.08110.34580.00410.357799.01474.8441-8.2852
183.961-0.8974-0.43992.6711-0.98841.3272-0.4175-0.234-0.41251.68920.02040.95420.6018-0.36550.31260.8969-0.04260.30860.44440.00130.616889.425312.14271.004
192.6494-0.86760.64774.3811-0.93535.5432-0.0952-0.278-0.01920.66310.04740.1049-0.2542-0.0440.04860.28810.00680.00440.30550.03510.4277101.6594-103.163-28.5298
202.09326.32394.01585.89931.05397.0507-0.456-0.22050.22250.39660.01331.03080.6543-1.22350.55030.3951-0.03330.17620.5919-0.11990.91185.8846-107.9573-32.3564
216.38236.51372.54046.58782.55370.9457-0.3456-0.07890.23771.3909-0.5683-0.3661-1.05020.31250.73551.2073-0.3543-0.32160.4970.1050.9436108.9765-85.5791-22.8072
226.9332-5.35375.17539.2871-5.15228.69750.01670.2860.31161.082-0.2890.7002-0.99090.24640.12350.6999-0.0844-0.06410.36440.00821.077899.1738-58.9333-41.5513
232.05871.15210.85410.69030.20833.5422-0.1525-0.42620.47272.29260.1048-0.2687-0.5458-0.0997-0.0841.591-0.1739-0.00730.5098-0.0510.6993103.3326-68.6196-28.7584
240.6604-0.38040.39396.01012.72012.05380.2955-0.65050.88620.8952-0.16010.0349-1.0304-0.0052-0.08941.0747-0.08980.22160.4319-0.0680.6914101.0249-71.7503-30.5873
252.2853-1.19792.13481.1606-1.64212.74870.151-0.72820.76291.3348-0.08330.4239-0.7867-0.359-0.07461.6029-0.09970.0710.5679-0.04611.0326102.7253-57.6115-29.2663
264.907-2.2792.94276.3551-2.19333.67650.0882-0.1870.30620.2687-0.0081-0.1566-0.56650.0723-0.01650.43710.0018-0.01860.3247-0.05960.215271.4688-2.51631.0454
272.1936-0.2545-1.96742.45460.64551.80440.1262-0.048-0.0622-0.3289-0.0599-0.07520.00140.1146-0.04970.44780.0442-0.05560.2969-0.00340.167371.3355-12.9968-1.0659
281.5838-0.3202-0.92536.5258-3.84942.5972-0.2147-0.0143-0.3846-0.85920.0229-0.03460.8582-0.01360.20360.72230.01790.01620.3778-0.01210.23866.9992-27.35486.8267
291.1139-0.3804-0.28663.6711.24972.64770.0651-0.02540.0947-0.42280.1475-0.1522-0.3351-0.0646-0.20140.2475-0.01250.02960.3231-0.01390.209363.941713.38811.7434
302.65460.82710.73882.23340.86877.29460.133-0.3532-0.0954-0.11990.3718-0.5418-0.14820.6994-0.47650.17250.0188-0.00430.3457-0.06650.380868.070915.995918.7926
318.9416-2.9424-4.59269.78588.46192.30480.1158-0.30540.6613-0.78021.1026-1.3974-0.6021.1451-1.15150.4346-0.04690.05240.42680.00050.449772.150120.808914.951
321.3559-0.12530.65242.53270.75133.84680.0630.0394-0.1285-0.167-0.03750.01780.3475-0.1547-0.01470.62590.03470.03070.39210.01270.191266.6934-35.592-26.4418
335.0227-1.047-0.67566.687-0.48794.68970.1397-0.1236-0.2682-1.05470.26230.46640.758-0.5276-0.37040.5782-0.0204-0.13930.34370.04060.353969.1016-65.381-11.9587
344.7005-1.90433.65066.5438-2.14085.0897-0.043-0.5039-0.9565-0.29220.19590.4613-0.0724-0.3067-0.08330.20050.00670.00060.3381-0.10580.4259106.955339.851-22.0324
352.14140.5345-1.37193.9429-0.23781.3297-0.0556-0.0095-0.08390.01130.0934-0.1321-0.13740.0577-0.04080.16160.024-0.07340.3356-0.06050.4011108.183951.4404-18.8429
361.52790.5839-0.37673.78270.00120.3204-0.11-0.04020.19040.01750.0618-0.0431-0.09310.00750.05480.18830.0283-0.06260.3936-0.03330.4201107.052249.3711-19.2319
371.4224-0.8399-1.20745.0666-0.05791.3708-0.076-0.39850.807-0.19840.4611-0.3081-0.51740.1345-0.22270.2204-0.06120.03730.391-0.23821.2221100.787568.0856-11.4854
384.5519-0.29521.09152.24220.63722.0282-0.0072-0.1914-0.18730.1314-0.1201-0.05670.0801-0.28010.13650.13090.0044-0.01320.3326-0.02210.182498.64921.7944-18.2346
393.83021.3370.18472.0306-0.31522.91610.05410.02920.07790.0022-0.09790.4584-0.0351-0.48240.0320.20680.0538-0.03560.3464-0.06980.35793.609121.6044-20.6544
401.9084-0.20250.89142.567-0.27581.53260.09630.15510.6207-0.00610.0680.4698-0.0846-0.0228-0.1470.2071-0.01760.06880.38130.08640.669986.0683-98.9855-46.2015
412.5545-0.6450.78241.4660.21480.91650.10450.0157-0.04390.06180.03270.5499-0.0184-0.1088-0.15030.2109-0.00660.07150.39030.07060.828989.5175-106.7793-42.9624
421.97451.7608-1.47925.4122-1.06982.13640.13110.10460.21350.42580.00320.8579-0.40470.0089-0.15830.34220.00530.03710.42380.10620.787794.8008-72.3227-44.7751
435.84322.49680.6964.6221-0.48834.60920.32020.14320.49170.7431-0.34640.704-0.32230.0941-0.03920.45410.03830.14930.32070.13090.761599.534-73.0905-43.5329
443.9559-1.0499-0.22050.92520.23563.086-0.0450.34340.32250.0037-0.06220.2869-0.02820.39380.10270.44420.00840.00240.44050.20070.6331101.2425-68.7167-52.7195
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN L AND RESID 3:107 )L3 - 107
2X-RAY DIFFRACTION2( CHAIN L AND RESID 108:138 )L108 - 138
3X-RAY DIFFRACTION3( CHAIN L AND RESID 139:212 )L139 - 212
4X-RAY DIFFRACTION4( CHAIN A AND RESID 3:75 )A3 - 75
5X-RAY DIFFRACTION5( CHAIN A AND RESID 76:95 )A76 - 95
6X-RAY DIFFRACTION6( CHAIN A AND RESID 96:151 )A96 - 151
7X-RAY DIFFRACTION7( CHAIN A AND RESID 152:162 )A152 - 162
8X-RAY DIFFRACTION8( CHAIN A AND RESID 163:191 )A163 - 191
9X-RAY DIFFRACTION9( CHAIN A AND RESID 192:210 )A192 - 210
10X-RAY DIFFRACTION10( CHAIN C AND RESID 3:61 )C3 - 61
11X-RAY DIFFRACTION11( CHAIN C AND RESID 62:75 )C62 - 75
12X-RAY DIFFRACTION12( CHAIN C AND RESID 76:91 )C76 - 91
13X-RAY DIFFRACTION13( CHAIN C AND RESID 92:102 )C92 - 102
14X-RAY DIFFRACTION14( CHAIN C AND RESID 103:114 )C103 - 114
15X-RAY DIFFRACTION15( CHAIN C AND RESID 115:150 )C115 - 150
16X-RAY DIFFRACTION16( CHAIN C AND RESID 151:174 )C151 - 174
17X-RAY DIFFRACTION17( CHAIN C AND RESID 175:191 )C175 - 191
18X-RAY DIFFRACTION18( CHAIN C AND RESID 192:209 )C192 - 209
19X-RAY DIFFRACTION19( CHAIN E AND RESID 3:91 )E3 - 91
20X-RAY DIFFRACTION20( CHAIN E AND RESID 92:102 )E92 - 102
21X-RAY DIFFRACTION21( CHAIN E AND RESID 103:114 )E103 - 114
22X-RAY DIFFRACTION22( CHAIN E AND RESID 115:130 )E115 - 130
23X-RAY DIFFRACTION23( CHAIN E AND RESID 131:151 )E131 - 151
24X-RAY DIFFRACTION24( CHAIN E AND RESID 152:181 )E152 - 181
25X-RAY DIFFRACTION25( CHAIN E AND RESID 182:209 )E182 - 209
26X-RAY DIFFRACTION26( CHAIN H AND RESID 1:17 )H1 - 17
27X-RAY DIFFRACTION27( CHAIN H AND RESID 18:87 )H18 - 87
28X-RAY DIFFRACTION28( CHAIN H AND RESID 88:103 )H88 - 103
29X-RAY DIFFRACTION29( CHAIN H AND RESID 104:175 )H104 - 175
30X-RAY DIFFRACTION30( CHAIN H AND RESID 176:203 )H176 - 203
31X-RAY DIFFRACTION31( CHAIN H AND RESID 204:215 )H204 - 215
32X-RAY DIFFRACTION32( CHAIN B AND RESID 1:119 )B1 - 119
33X-RAY DIFFRACTION33( CHAIN B AND RESID 120:213 )B120 - 213
34X-RAY DIFFRACTION34( CHAIN D AND RESID 1:17 )D1 - 17
35X-RAY DIFFRACTION35( CHAIN D AND RESID 18:72 )D18 - 72
36X-RAY DIFFRACTION36( CHAIN D AND RESID 73:100A )D73 - 100
37X-RAY DIFFRACTION37( CHAIN D AND RESID 100B:103 )D101 - 103
38X-RAY DIFFRACTION38( CHAIN D AND RESID 104:143 )D104 - 143
39X-RAY DIFFRACTION39( CHAIN D AND RESID 144:214 )D144 - 214
40X-RAY DIFFRACTION40( CHAIN F AND RESID 1:72 )F1 - 72
41X-RAY DIFFRACTION41( CHAIN F AND RESID 73:103 )F73 - 103
42X-RAY DIFFRACTION42( CHAIN F AND RESID 104:143 )F104 - 143
43X-RAY DIFFRACTION43( CHAIN F AND RESID 144:188 )F144 - 188
44X-RAY DIFFRACTION44( CHAIN F AND RESID 189:215 )F189 - 215

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