+Open data
-Basic information
Entry | Database: PDB / ID: 2xnd | ||||||
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Title | Crystal structure of bovine F1-c8 sub-complex of ATP Synthase | ||||||
Components |
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Keywords | HYDROLASE / ATP PHOSPHORYLASE (H+ TRANSPORTING) / ATP SYNTHESIS / F1FO ATP SYNTHASE / ION TRANSPORT / P-LOOP | ||||||
Function / homology | Function and homology information Mitochondrial protein import / Formation of ATP by chemiosmotic coupling / Cristae formation / mitochondrial proton-transporting ATP synthase complex assembly / mitochondrial envelope / mitochondrial proton-transporting ATP synthase, catalytic core / proton-transporting ATP synthase complex / mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) / mitochondrial proton-transporting ATP synthase complex / mitochondrial proton-transporting ATP synthase complex, catalytic sector F(1) ...Mitochondrial protein import / Formation of ATP by chemiosmotic coupling / Cristae formation / mitochondrial proton-transporting ATP synthase complex assembly / mitochondrial envelope / mitochondrial proton-transporting ATP synthase, catalytic core / proton-transporting ATP synthase complex / mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) / mitochondrial proton-transporting ATP synthase complex / mitochondrial proton-transporting ATP synthase complex, catalytic sector F(1) / proton motive force-driven mitochondrial ATP synthesis / proton motive force-driven ATP synthesis / proton transmembrane transporter activity / proton-transporting ATP synthase complex, catalytic core F(1) / aerobic respiration / H+-transporting two-sector ATPase / proton-transporting ATPase activity, rotational mechanism / proton transmembrane transport / proton-transporting ATP synthase activity, rotational mechanism / ADP binding / mitochondrial inner membrane / lipid binding / ATP hydrolysis activity / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | BOS TAURUS (cattle) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.5 Å | ||||||
Authors | Watt, I.N. / Montgomery, M.G. / Runswick, M.J. / Leslie, A.G.W. / Walker, J.E. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2010 Title: Bioenergetic Cost of Making an Adenosine Triphosphate Molecule in Animal Mitochondria. Authors: Watt, I.N. / Montgomery, M.G. / Runswick, M.J. / Leslie, A.G.W. / Walker, J.E. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 10-STRANDED BARREL THIS IS REPRESENTED BY A 11-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "DA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 6-STRANDED BARREL THIS IS REPRESENTED BY A 7-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2xnd.cif.gz | 689.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2xnd.ent.gz | 556.2 KB | Display | PDB format |
PDBx/mmJSON format | 2xnd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xn/2xnd ftp://data.pdbj.org/pub/pdb/validation_reports/xn/2xnd | HTTPS FTP |
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-Related structure data
Related structure data | 1e79S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-ATP SYNTHASE SUBUNIT ... , 5 types, 9 molecules ABCDEFGHI
#1: Protein | Mass: 53386.066 Da / Num. of mol.: 3 / Fragment: RESIDUES 62-553 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART / Tissue: MUSCLESkeletal muscle References: UniProt: P19483, H+-transporting two-sector ATPase #2: Protein | Mass: 50365.371 Da / Num. of mol.: 3 / Fragment: RESIDUES 59-525 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART / Tissue: MUSCLESkeletal muscle References: UniProt: P00829, H+-transporting two-sector ATPase #3: Protein | | Mass: 30185.674 Da / Num. of mol.: 1 / Fragment: RESIDUES 26-297 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART / Tissue: MUSCLESkeletal muscle References: UniProt: P05631, H+-transporting two-sector ATPase #4: Protein | | Mass: 13811.496 Da / Num. of mol.: 1 / Fragment: RESIDUES 37-167 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART / Tissue: MUSCLESkeletal muscle References: UniProt: P05630, H+-transporting two-sector ATPase #5: Protein/peptide | | Mass: 5275.220 Da / Num. of mol.: 1 / Fragment: RESIDUES 2-48 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART / Tissue: MUSCLESkeletal muscle References: UniProt: P05632, H+-transporting two-sector ATPase |
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-Protein , 1 types, 8 molecules JKLMNOPQ
#6: Protein | Mass: 7293.593 Da / Num. of mol.: 8 / Fragment: RESIDUES 63-134 / Source method: isolated from a natural source / Source: (natural) BOS TAURUS (cattle) / Organ: HEART / Tissue: MUSCLESkeletal muscle References: UniProt: P32876, H+-transporting two-sector ATPase |
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-Non-polymers , 4 types, 12 molecules
#7: Chemical | ChemComp-ANP / #8: Chemical | ChemComp-MG / #9: Chemical | ChemComp-GOL / | #10: Chemical | ChemComp-SO4 / | |
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-Details
Sequence details | DIFFERENT RESIDUE: GLY A 481, GLY B 481, GLY C 481 THIS RESIDUE WAS IDENTIFIED AS A GLY FROM THE ...DIFFERENT RESIDUE: GLY A 481, GLY B 481, GLY C 481 THIS RESIDUE WAS IDENTIFIED |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.17 Å3/Da / Density % sol: 61.16 % / Description: NONE |
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Crystal grow | pH: 7 Details: CRYSTALS WERE GROWN UNDER OIL BY MIXING EQUAL VOLUMES OF PROTEIN (10MG/ML IN 20MM TRIS PH 8.0, 10% GLYCEROL, 1MM ADP, 1MM AMP-PNP, 2MM MGSO4, 0.02% NAN3, 5.7MM TDM) AND PRECIPITANT SOLUTION ...Details: CRYSTALS WERE GROWN UNDER OIL BY MIXING EQUAL VOLUMES OF PROTEIN (10MG/ML IN 20MM TRIS PH 8.0, 10% GLYCEROL, 1MM ADP, 1MM AMP-PNP, 2MM MGSO4, 0.02% NAN3, 5.7MM TDM) AND PRECIPITANT SOLUTION (50MM HEPES PH 7.0, 14% PEG4600, 50MM K2HPO4) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1.0007 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: May 2, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0007 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→132.6 Å / Num. obs: 72959 / % possible obs: 99.6 % / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Biso Wilson estimate: 71.804 Å2 / Rmerge(I) obs: 0.26 / Net I/σ(I): 3.7 |
Reflection shell | Resolution: 3.5→3.59 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.82 / Mean I/σ(I) obs: 1.6 / % possible all: 99.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1E79 Resolution: 3.5→132.6 Å / Cor.coef. Fo:Fc: 0.851 / Cor.coef. Fo:Fc free: 0.819 / SU B: 39.542 / SU ML: 0.602 / Cross valid method: THROUGHOUT / ESU R: 0 / ESU R Free: 0.674 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 80.15 Å2
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Refinement step | Cycle: LAST / Resolution: 3.5→132.6 Å
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