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- PDB-2x9h: CRYSTAL STRUCTURE OF MYOSIN-2 MOTOR DOMAIN IN COMPLEX WITH ADP- M... -
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Basic information
Entry | Database: PDB / ID: 2x9h | ||||||
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Title | CRYSTAL STRUCTURE OF MYOSIN-2 MOTOR DOMAIN IN COMPLEX WITH ADP- METAVANADATE AND PENTACHLOROCARBAZOLE | ||||||
![]() | MYOSIN-2 HEAVY CHAIN | ||||||
![]() | CONTRACTILE PROTEIN / NUCLEOTIDE-BINDING / MOTOR PROTEIN / ACTIN-BINDING | ||||||
Function / homology | ![]() calcium-dependent ATPase activity / pseudopodium retraction / uropod retraction / cytoplasmic actin-based contraction involved in forward cell motility / phagocytic cup base / pathogen-containing vacuole / equatorial cell cortex / contractile actin filament bundle assembly / cell trailing edge / contractile vacuole organization ...calcium-dependent ATPase activity / pseudopodium retraction / uropod retraction / cytoplasmic actin-based contraction involved in forward cell motility / phagocytic cup base / pathogen-containing vacuole / equatorial cell cortex / contractile actin filament bundle assembly / cell trailing edge / contractile vacuole organization / myosin filament assembly / aggregation involved in sorocarp development / culmination involved in sorocarp development / RHO GTPases activate PAKs / adenyl nucleotide binding / response to differentiation-inducing factor 1 / hypotonic response / uropod / mitotic actomyosin contractile ring / actomyosin contractile ring / mitotic actomyosin contractile ring contraction / actin-myosin filament sliding / detection of mechanical stimulus / apical cortex / negative regulation of actin filament polymerization / bleb assembly / actomyosin / substrate-dependent cell migration, cell extension / myosin filament / filopodium assembly / early phagosome / cortical actin cytoskeleton organization / myosin II complex / cortical actin cytoskeleton / microfilament motor activity / pseudopodium / cytoskeletal motor activity / cleavage furrow / mitotic cytokinesis / response to mechanical stimulus / 14-3-3 protein binding / response to cAMP / extracellular matrix / muscle contraction / cell motility / response to hydrogen peroxide / protein localization / chemotaxis / actin filament binding / cell cortex / regulation of cell shape / cytoplasmic vesicle / cytoskeleton / calmodulin binding / ATP binding / identical protein binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Selvadurai, J. / Kirst, J. / Knoelker, H.J. / Manstein, D.J. | ||||||
![]() | ![]() Title: Crystal Structure of Myosin-2 Motor Domain in Complex with Adp-Metavanadate and Pentachlorocarbazole Authors: Fedrov, R. / Boehl, M. / Tsiavaliaris, G. / Hartmann, F.K. / Baruch, P. / Brenner, B. / Martin, R. / Knoelker, H.J. / Gutzeit, H.O. / Manstein, D.J. #1: ![]() Title: The Mechanism of Pentabromopseudilin Inhibition of Myosin Motor Activity. Authors: Fedorov, R. / Bohl, M. / Tsiavaliaris, G. / Hartmann, F.K. / Taft, M.H. / Baruch, P. / Brenner, B. / Martin, R. / Knolker, H. / Gutzeit, H.O. / Manstein, D.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 156.7 KB | Display | ![]() |
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PDB format | ![]() | 122.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 991.3 KB | Display | ![]() |
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Full document | ![]() | 1006.3 KB | Display | |
Data in XML | ![]() | 28.8 KB | Display | |
Data in CIF | ![]() | 40.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2jhrS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 78941.242 Da / Num. of mol.: 1 / Fragment: MYOSIN MOTOR DOMAIN, RESIDUES 2-696 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-AD9 / |
#3: Chemical | ChemComp-MG / |
#4: Chemical | ChemComp-KI9 / |
#5: Water | ChemComp-HOH / |
Nonpolymer details | ADP METAVANADA |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.21 Å3/Da / Density % sol: 61 % / Description: NONE |
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Crystal grow | pH: 7.4 Details: 50 MM TRIS-HCL PH 7.4, 140 MM NACL, 9% W/V PEG8000, 2% (V/V) MPD, 5 MM MGCL2, 5 MM DTT, 1 MM EGTA |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Feb 24, 2010 Details: SINGLE SILICON (111) MONOCHROMATOR AND TOROIDAL FOCUSING MIRROR |
Radiation | Monochromator: SINGLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.939 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→19.77 Å / Num. obs: 26158 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 5 % / Biso Wilson estimate: 38 Å2 / Rmerge(I) obs: 0.4 / Net I/σ(I): 2.4 |
Reflection shell | Resolution: 2.7→2.769 Å / % possible all: 100 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2JHR Resolution: 2.7→19.77 Å / Cor.coef. Fo:Fc: 0.907 / Cor.coef. Fo:Fc free: 0.826 / SU B: 0.001 / SU ML: 0 / Cross valid method: THROUGHOUT / ESU R: 0.263 / ESU R Free: 0.373 Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.RESIDUES 202-207 ARE DISORDERED. DISORDERED REGIONS COULD NOT BE MODELED DUE TO POOR DENSITY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||
Displacement parameters | Biso mean: 20.946 Å2
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Refinement step | Cycle: LAST / Resolution: 2.7→19.77 Å
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LS refinement shell | Resolution: 2.7→2.769 Å / Total num. of bins used: 20
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