Hydrolases; Acting on peptide bonds (peptidases); Metalloendopeptidases / metalloendopeptidase activity / extracellular region / metal ion binding Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 277 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, GLU 163 TO ALA
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.15 Å3/Da / Density % sol: 42.8 % / Description: NONE
Crystal grow
Method: vapor diffusion, hanging drop / pH: 4.6 Details: THE HANGING-DROP VAPOR DIFFUSION METHOD AGAINST A PRECIPITANT SOLUTION OF 15% PEG MME 2000, 0.2M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE PH 4.6. DROPLETS CONTAINED 3 MKL OF 10 MG/ML PROTEIN ...Details: THE HANGING-DROP VAPOR DIFFUSION METHOD AGAINST A PRECIPITANT SOLUTION OF 15% PEG MME 2000, 0.2M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE PH 4.6. DROPLETS CONTAINED 3 MKL OF 10 MG/ML PROTEIN SOLUTION AND 1.5 MKL PRECIPITANT SOLUTION WITH 0.06% BETA-OCTIL-D-GLUCOPYRANOSIDE IN THE SAME BUFFER
Resolution: 1.821→52.63 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.933 / SU B: 2.09 / SU ML: 0.067 / Cross valid method: THROUGHOUT / ESU R: 0.135 / ESU R Free: 0.123 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.20205
1448
5.1 %
RANDOM
Rwork
0.16984
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obs
0.17141
27076
94.2 %
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Solvent computation
Ion probe radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT