+Open data
-Basic information
Entry | Database: PDB / ID: 2pe8 | ||||||
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Title | Crystal structure of the UHM domain of human SPF45 (free form) | ||||||
Components | Splicing factor 45 | ||||||
Keywords | PROTEIN BINDING / RRM | ||||||
Function / homology | Function and homology information alternative mRNA splicing, via spliceosome / mRNA cis splicing, via spliceosome / mRNA Splicing - Major Pathway / spliceosomal complex / RNA binding / nucleoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Corsini, L. / Basquin, J. / Hothorn, M. / Sattler, M. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2007 Title: U2AF-homology motif interactions are required for alternative splicing regulation by SPF45. Authors: Corsini, L. / Bonna, S. / Basquin, J. / Hothorn, M. / Scheffzek, K. / Valcarcel, J. / Sattler, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2pe8.cif.gz | 32.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2pe8.ent.gz | 21.3 KB | Display | PDB format |
PDBx/mmJSON format | 2pe8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pe/2pe8 ftp://data.pdbj.org/pub/pdb/validation_reports/pe/2pe8 | HTTPS FTP |
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-Related structure data
Related structure data | 2pehC 1o0pS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 11690.481 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RBM17, SPF45 / Plasmid: pETM30 / Production host: Escherichia coli (E. coli) / Strain (production host): K12 BL21(DE3) pLysS / References: UniProt: Q96I25 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.05 Å3/Da / Density % sol: 59.71 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 22% PEG 3,350, 0.2 M MgCl2, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.976 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Sep 1, 2005 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976 Å / Relative weight: 1 |
Reflection | Resolution: 2→15 Å / Num. all: 9819 / Num. obs: 9819 / % possible obs: 99.5 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Redundancy: 7.57 % / Biso Wilson estimate: 46.5 Å2 / Rmerge(I) obs: 0.057 / Rsym value: 0.053 / Net I/σ(I): 20.13 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.474 / Mean I/σ(I) obs: 4.67 / Num. unique all: 719 / Rsym value: 0.44 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: UHM domain homology model based on PDB-ID 1o0p Resolution: 2→19.58 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.956 / SU B: 12.328 / SU ML: 0.169 / TLS residual ADP flag: LIKELY RESIDUAL / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): -3 / σ(I): -3 / ESU R: 0.169 / ESU R Free: 0.145 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.965 Å2
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Refinement step | Cycle: LAST / Resolution: 2→19.58 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.052 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Origin x: 15.6378 Å / Origin y: -6.1278 Å / Origin z: -11.5434 Å
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