+Open data
-Basic information
Entry | Database: PDB / ID: 2ond | ||||||
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Title | Crystal Structure of the HAT-C domain of murine CstF-77 | ||||||
Components | Cleavage stimulation factor 77 kDa subunit | ||||||
Keywords | STRUCTURAL PROTEIN / HAT domain | ||||||
Function / homology | Function and homology information Processing of Intronless Pre-mRNAs / Processing of Capped Intron-Containing Pre-mRNA / mRNA cleavage stimulating factor complex / mRNA 3'-end processing / RNA Polymerase II Transcription Termination / co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway / RNA 3'-end processing / mRNA binding / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.8 Å | ||||||
Authors | Bai, Y. / Auperin, T.C. / Chou, C.-Y. / Chang, G.-G. / Manley, J.L. / Tong, L. | ||||||
Citation | Journal: Mol.Cell / Year: 2007 Title: Crystal Structure of Murine CstF-77: Dimeric Association and Implications for Polyadenylation of mRNA Precursors. Authors: Bai, Y. / Auperin, T.C. / Chou, C.Y. / Chang, G.G. / Manley, J.L. / Tong, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ond.cif.gz | 133.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ond.ent.gz | 107.6 KB | Display | PDB format |
PDBx/mmJSON format | 2ond.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/on/2ond ftp://data.pdbj.org/pub/pdb/validation_reports/on/2ond | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36632.531 Da / Num. of mol.: 2 / Fragment: residues 242-549 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Cstf3 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta / References: UniProt: Q99LI7 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.84 Å3/Da / Density % sol: 68 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: with protease (subtilisin) in the drop, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 0.9798 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Oct 15, 2005 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9798 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→30 Å / Num. obs: 53540 / % possible obs: 99.5 % / Biso Wilson estimate: 40.3 Å2 / Rmerge(I) obs: 0.069 / Net I/σ(I): 35.5688 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 9.6 % / Rmerge(I) obs: 0.413 / Mean I/σ(I) obs: 6.3 / % possible all: 99.7 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.8→29.84 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 360403.58 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 35.3067 Å2 / ksol: 0.350414 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 56.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.8→29.84 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.9 Å / Rfactor Rfree error: 0.022 / Total num. of bins used: 10
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Xplor file |
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