+Open data
-Basic information
Entry | Database: PDB / ID: 2ooe | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of HAT domain of murine CstF-77 | ||||||
Components | Cleavage stimulation factor 77 kDa subunit | ||||||
Keywords | STRUCTURAL PROTEIN / HAT domain | ||||||
Function / homology | Function and homology information Processing of Intronless Pre-mRNAs / Processing of Capped Intron-Containing Pre-mRNA / mRNA cleavage stimulating factor complex / mRNA 3'-end processing / RNA Polymerase II Transcription Termination / co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway / RNA 3'-end processing / mRNA binding / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3 Å | ||||||
Authors | Bai, Y. / Auperin, T.C. / Chou, C.-Y. / Chang, G.-G. / Manley, J.L. / Tong, L. | ||||||
Citation | Journal: Mol.Cell / Year: 2007 Title: Crystal Structure of Murine CstF-77: Dimeric Association and Implications for Polyadenylation of mRNA Precursors. Authors: Bai, Y. / Auperin, T.C. / Chou, C.Y. / Chang, G.G. / Manley, J.L. / Tong, L. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2ooe.cif.gz | 120.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2ooe.ent.gz | 94 KB | Display | PDB format |
PDBx/mmJSON format | 2ooe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oo/2ooe ftp://data.pdbj.org/pub/pdb/validation_reports/oo/2ooe | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Details | The biological assembly is a dimer generated from the monomer in the asymmetric unit. |
-Components
#1: Protein | Mass: 62635.914 Da / Num. of mol.: 1 / Fragment: residues 21-549 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Cstf3 / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta / References: UniProt: Q99LI7 |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 3.07 Å3/Da / Density % sol: 59.95 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 50mM Bis-tris, 50mM ammonium sulfate, 30%(v/v) pentaerythritol ethoxylate(15/4EO/OH), pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 0.9798 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9798 Å / Relative weight: 1 |
Reflection | Resolution: 3→30 Å / Num. obs: 15612 / % possible obs: 98.1 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.071 / Net I/σ(I): 16.1463 |
Reflection shell | Resolution: 3→3.11 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.199 / Mean I/σ(I) obs: 5.691 / % possible all: 99.4 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: SAD / Resolution: 3→27.17 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 1270902.46 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL / Bsol: 22.246 Å2 / ksol: 0.29289 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 56.4 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→27.17 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 3→3.11 Å / Rfactor Rfree error: 0.036 / Total num. of bins used: 10
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
|