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- PDB-2mgz: Solution structure of RBFOX family ASD-1 RRM and SUP-12 RRM in te... -
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Basic information
Entry | Database: PDB / ID: 2mgz | ||||||
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Title | Solution structure of RBFOX family ASD-1 RRM and SUP-12 RRM in ternary complex with RNA | ||||||
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![]() | RNA BINDING PROTEIN/RNA / Solution Structure / Protein-RNA complex / Ternary Complex / RRM (RNA recognition motif) / RNA BINDING PROTEIN-RNA complex | ||||||
Function / homology | ![]() miRNA transport / alternative mRNA splicing, via spliceosome / pre-mRNA binding / regulation of locomotion / regulation of alternative mRNA splicing, via spliceosome / pre-mRNA intronic binding / mRNA 3'-UTR binding / regulation of actin cytoskeleton organization / nervous system development / single-stranded RNA binding ...miRNA transport / alternative mRNA splicing, via spliceosome / pre-mRNA binding / regulation of locomotion / regulation of alternative mRNA splicing, via spliceosome / pre-mRNA intronic binding / mRNA 3'-UTR binding / regulation of actin cytoskeleton organization / nervous system development / single-stranded RNA binding / nuclear speck / ribonucleoprotein complex / mRNA binding / mitochondrion / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | fewest violations, model1 | ||||||
![]() | Takahashi, M. / Kuwasako, K. / Unzai, S. / Tsuda, K. / Yoshikawa, S. / He, F. / Kobayashi, N. / Guntert, P. / Shirouzu, M. / Ito, T. ...Takahashi, M. / Kuwasako, K. / Unzai, S. / Tsuda, K. / Yoshikawa, S. / He, F. / Kobayashi, N. / Guntert, P. / Shirouzu, M. / Ito, T. / Tanaka, A. / Yokoyama, S. / Hagiwara, M. / Kuroyanagi, H. / Muto, Y. | ||||||
![]() | ![]() Title: RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing Authors: Kuwasako, K. / Takahashi, M. / Unzai, S. / Tsuda, K. / Yoshikawa, S. / He, F. / Kobayashi, N. / Guntert, P. / Shirouzu, M. / Ito, T. / Tanaka, A. / Yokoyama, S. / Hagiwara, M. / Kuroyanagi, H. / Muto, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.3 MB | Display | ![]() |
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PDB format | ![]() | 1.1 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 465.7 KB | Display | ![]() |
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Full document | ![]() | 815.1 KB | Display | |
Data in XML | ![]() | 86.6 KB | Display | |
Data in CIF | ![]() | 114.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10588.028 Da / Num. of mol.: 1 / Fragment: UNP residues 97-189 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 11611.009 Da / Num. of mol.: 1 / Fragment: UNP residues 20-123 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: RNA chain | Mass: 3845.303 Da / Num. of mol.: 1 / Source method: obtained synthetically |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 0.7 mM [U-99% 13C; U-99% 15N] ASD-1-1, 0.7 mM [U-99% 13C; U-99% 15N] SUP-12-2, 0.7 mM RNA-3, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.1 / pH: 5-7 / Pressure: ambient / Temperature: 288 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software | Name: ![]() Developer: Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: fewest violations |
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 400 / Conformers submitted total number: 20 / Representative conformer: 1 |