- PDB-2lf3: Solution NMR structure of HopPmaL_281_385 from Pseudomonas syring... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2lf3
Title
Solution NMR structure of HopPmaL_281_385 from Pseudomonas syringae pv. maculicola str. ES4326, Midwest Center for Structural Genomics target APC40104.5 and Northeast Structural Genomics Consortium target PsT2A
Components
Effector protein hopAB3
Keywords
SIGNALING PROTEIN / type III effector / Structural Genomics / PSI-Biology / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG / Midwest Center for Structural Genomics / MCSG / Ontario Centre for Structural Proteomics / OCSP
Function / homology
Function and homology information
symbiont-mediated perturbation of host defense-related programmed cell death / extracellular region Similarity search - Function
EffectorproteinhopAB3 / Avirulence protein hopPmaL
Mass: 11767.334 Da / Num. of mol.: 1 / Fragment: sequence database residues 281-385 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas syringae pv. maculicola (bacteria) Gene: hopAB3, hopPmaL / Production host: Escherichia coli (E. coli) / References: UniProt: Q8RP04
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
3D HNCO
1
2
1
3DCBCA(CO)NH
1
3
1
3DHBHA(CO)NH
1
4
1
3D HNCA
1
5
1
3D (H)CCH-TOCSY
1
6
1
3D CCH-TOCSY
1
7
1
3D 1H-15N NOESY
1
8
2
3D 1H-15N NOESY
1
9
1
3D 1H-13C NOESY
1
10
2
3D 1H-13C NOESY
1
11
1
3D 1H-13C NOESY aromatic
1
12
2
3D 1H-13C NOESY aromatic
1
13
3
2D 1H-13C HSQC
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.3 mM [U-100% 13C; U-100% 15N] HopPmaL_281_385, 10 mM [U-100% 2H] TRIS, 300 mM sodium chloride, 10 uM zinc sulphate, 10 mM [U-100% 2H] DTT, 0.01 % NaN3, 10 mM benzamidine, 1 x inhibitor cocktail, 90% H2O/10% D2O
90% H2O/10% D2O
2
1 mM [U-100% 13C; U-100% 15N] HopPmaL,_281_385, 10 mM [U-100% 2H] TRIS 300 mM sodium chloride, 10 uM zinc sulphate, 10 mM [U-100% 2H] DTT, 0.01 % NaN3, 10 mM benzamidine, 1 x inhibitor cocktail, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.2 mM [U-7% 13C; U-100% 15N] HopPmaL_281_385, 10 mM [U-100% 2H] TRIS, 300 mM sodium chloride, 10 uM zinc sulphate, 10 mM [U-100% 2H] DTT, 0.01 % NaN3, 10 mM benzamidine, 1 x inhibitor cocktail, 90% H2O/10% D2O
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