MEMBRANE PROTEIN / CHROMOPHORE / CHLORIDE PUMP / ION TRANSPORT / MEMBRANE / CHLORIDE / RECEPTOR / ION PUMP / TRANSPORT / SENSORY TRANSDUCTION / PHOTORECEPTOR PROTEIN / TRANSMEMBRANE / RETINAL PROTEIN
Function / homology
Function and homology information
photoreceptor activity / phototransduction / monoatomic ion channel activity / plasma membrane Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 67 / Source method: isolated from a natural source / Formula: H2O
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Details
Compound details
ENGINEERED RESIDUE IN CHAIN A, THR 203 TO VAL ENGINEERED RESIDUE IN CHAIN A, VAL 229 TO ALA
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 2.34 Å3/Da / Density % sol: 47.4 %
Crystal grow
Method: lipidic cubic phase Details: CRYSTALLIZED IN A CUBIC LIPID PHASE MADE OF 58-62 W/V % 1-MONOOLEIN, 3.3 M KCL, 3.5 MG/ML HR AND 25 MM TRIS/HCL, PH 7, R.T.
Resolution: 1.7→20 Å / Data cutoff high absF: 10000 / Cross valid method: THROUGHOUT / σ(F): 2.4 Details: 3-10 HELIX DISTORTION BETWEEN LEU A 110 AND ALA A 113. PI-BULGE BETWEEN ALA A 178 AND TRP A 183 DISRUPTS HELIX E
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2636
1754
5 %
RANDOM
Rwork
0.238
-
-
-
obs
0.238
35082
99.8 %
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Solvent computation
Bsol: 76.7206 Å2 / ksol: 0.388698 e/Å3
Displacement parameters
Baniso -1
Baniso -2
Baniso -3
1-
1.349 Å2
0.034 Å2
0 Å2
2-
-
1.349 Å2
0 Å2
3-
-
-
-2.699 Å2
Refinement step
Cycle: LAST / Resolution: 1.7→20 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
1822
0
78
67
1967
Refine LS restraints
Refine-ID
Type
Dev ideal
X-RAY DIFFRACTION
c_bond_d
0.01
X-RAY DIFFRACTION
c_bond_d_na
X-RAY DIFFRACTION
c_bond_d_prot
X-RAY DIFFRACTION
c_angle_d
X-RAY DIFFRACTION
c_angle_d_na
X-RAY DIFFRACTION
c_angle_d_prot
X-RAY DIFFRACTION
c_angle_deg
1.2
X-RAY DIFFRACTION
c_angle_deg_na
X-RAY DIFFRACTION
c_angle_deg_prot
X-RAY DIFFRACTION
c_dihedral_angle_d
X-RAY DIFFRACTION
c_dihedral_angle_d_na
X-RAY DIFFRACTION
c_dihedral_angle_d_prot
X-RAY DIFFRACTION
c_improper_angle_d
X-RAY DIFFRACTION
c_improper_angle_d_na
X-RAY DIFFRACTION
c_improper_angle_d_prot
X-RAY DIFFRACTION
c_mcbond_it
X-RAY DIFFRACTION
c_mcangle_it
X-RAY DIFFRACTION
c_scbond_it
X-RAY DIFFRACTION
c_scangle_it
+
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