#1: Journal: Curr.Opin.Struct.Biol. / Year: 1998 Title: The Structure and Mechanism of the Family of Retinal Proteins from Halophilic Archaea Authors: Oesterhelt, D.
#2: Journal: J.Mol.Biol. / Year: 1995 Title: Three-Dimensional Structure of Halorhodopsin at 7 Angstrom Resolution Authors: Havelka, W. / Henderson, R. / Oesterhelt, D.
History
Deposition
Apr 14, 2000
Deposition site: PDBE / Processing site: PDBE
Revision 1.0
Jun 2, 2000
Provider: repository / Type: Initial release
Revision 1.1
Nov 14, 2012
Group: Atomic model / Database references ...Atomic model / Database references / Derived calculations / Non-polymer description / Other / Refinement description / Version format compliance
Revision 1.2
Feb 3, 2016
Group: Atomic model / Database references ...Atomic model / Database references / Derived calculations / Non-polymer description / Other / Refinement description / Source and taxonomy / Structure summary
Mass: 18.015 Da / Num. of mol.: 96 / Source method: isolated from a natural source / Formula: H2O
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Details
Compound details
CHAIN A ENGINEERED MUTATION VAL229ALA
Sequence details
ALA A 229, MUTATION IN D2 STRAIN VERIFIED BY DIDEOXY-SEQUENCING C-TERMINUS NOT DEFINED IN ELECTRON ...ALA A 229, MUTATION IN D2 STRAIN VERIFIED BY DIDEOXY-SEQUENCING C-TERMINUS NOT DEFINED IN ELECTRON DENSITY FROM A263 - D274. IN THE CRYSTALS, HR ASSEMBLES SIMILARLY TO HOMOTRIMERS AS BACTERIORHODOPSIN.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.54 Å3/Da / Density % sol: 41.5 %
Crystal grow
Method: lipidic cubic phase / pH: 7 Details: CRYSTALLIZED IN A CUBIC LIPID PHASE MADE OF 58-62 W/V % 1-MONOOLEIN, 4 M KCL, 3.3-4.0 MG/ML HR AND 50 MM TRIS/HCL, PH 7.
Resolution: 1.8→25 Å / Data cutoff high absF: 10000 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 Details: LIPID PATCH BETWEEN HR TRIMERS MODELLED WITH 1-MONOOLEIN MOLECULES. NOTE THAT THESE OLC MOLECULES ARE MOSTLY ONLY PARTIALLY DEFINED BY ELECTRON DENSITY.
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