+Open data
-Basic information
Entry | Database: PDB / ID: 2bsb | ||||||
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Title | E. coli F17e-G lectin domain complex with N-acetylglucosamine | ||||||
Components | F17G ADHESIN SUBUNIT | ||||||
Keywords | LECTIN / BACTERIAL ADHESION / PROTEIN-SUGAR COMPLEX / FIMBRIAE | ||||||
Function / homology | Function and homology information adhesion of symbiont to host / pilus / : / carbohydrate binding / cell adhesion Similarity search - Function | ||||||
Biological species | ESCHERICHIA COLI (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Buts, L. / Wellens, A. / Van Molle, I. / Wyns, L. / Loris, R. / Lahmann, M. / Oscarson, S. / De Greve, H. / Bouckaert, J. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2005 Title: Impact of Natural Variation in Bacterial F17G Adhesins on Crystallization Behaviour. Authors: Buts, L. / Wellens, A. / Van Molle, I. / Wyns, L. / Loris, R. / Lahmann, M. / Oscarson, S. / De Greve, H. / Bouckaert, J. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2bsb.cif.gz | 45.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2bsb.ent.gz | 30.5 KB | Display | PDB format |
PDBx/mmJSON format | 2bsb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bs/2bsb ftp://data.pdbj.org/pub/pdb/validation_reports/bs/2bsb | HTTPS FTP |
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-Related structure data
Related structure data | 1zk5C 1zplC 2bs7C 2bs8C 2bscC 1o9vS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18936.871 Da / Num. of mol.: 1 / Fragment: LECTIN DOMAIN, RESIDUES 23-198 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ESCHERICHIA COLI (E. coli) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q9RH92 |
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#2: Sugar | ChemComp-NAG / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 50 % |
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Crystal grow | pH: 4.6 / Details: 30% PEG 400, 0.1 M CDCL2, 100 MM NA ACETATE PH 4.6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: X11 / Wavelength: 0.8128 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jul 1, 2004 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8128 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. obs: 9212 / % possible obs: 99.7 % / Observed criterion σ(I): 0 / Redundancy: 14.5 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 22.64 |
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 14.7 % / Rmerge(I) obs: 0.79 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1O9V (CHAIN A) Resolution: 2.4→50 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.4→50 Å
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Refine LS restraints |
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