+Open data
-Basic information
Entry | Database: PDB / ID: 1zpl | ||||||
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Title | E. coli F17a-G lectin domain complex with GlcNAc(beta1-O)Me | ||||||
Components | F17a-G | ||||||
Keywords | CELL ADHESION / FIMBRIAE / LECTINS / BACTERIAL ADHESION / PROTEIN-SUGAR COMPLEX | ||||||
Function / homology | Function and homology information adhesion of symbiont to host / pilus / carbohydrate binding / cell adhesion Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Buts, L. / Wellens, A. / Van Molle, I. / Wyns, L. / Loris, R. / Lahmann, M. / Oscarson, S. / De Greve, H. / Bouckaert, J. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2005 Title: Impact of natural variation in bacterial F17G adhesins on crystallization behaviour. Authors: Buts, L. / Wellens, A. / Van Molle, I. / Wyns, L. / Loris, R. / Lahmann, M. / Oscarson, S. / De Greve, H. / Bouckaert, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zpl.cif.gz | 89 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zpl.ent.gz | 66 KB | Display | PDB format |
PDBx/mmJSON format | 1zpl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zp/1zpl ftp://data.pdbj.org/pub/pdb/validation_reports/zp/1zpl | HTTPS FTP |
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-Related structure data
Related structure data | 1zk5C 2bs7C 2bs8C 2bsbC 2bscC 1o9vS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 19048.227 Da / Num. of mol.: 2 / Fragment: lectin domain (residues 23-199) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Description: expression vector with a T7 promotor / Gene: F17G AF022140 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-AI / References: UniProt: Q99003 #2: Sugar | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 10% (v/v) isopropanol, 20% (w/v) PEG 4000, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 1, 2004 |
Radiation | Monochromator: ESRF ID14-1 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.7→50 Å / Num. obs: 35422 / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 1.7→1.76 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.335 / % possible all: 70 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB 1O9V CHAIN A Resolution: 1.7→20 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.7→20 Å
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Refine LS restraints |
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