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- PDB-2b3o: Crystal structure of human tyrosine phosphatase SHP-1 -

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Basic information

Entry
Database: PDB / ID: 2b3o
TitleCrystal structure of human tyrosine phosphatase SHP-1
ComponentsTyrosine-protein phosphatase, non-receptor type 6
KeywordsHYDROLASE / Protein Tyrosine Phosphatase / SHP-1 / Signaling
Function / homology
Function and homology information


negative regulation of humoral immune response mediated by circulating immunoglobulin / negative regulation of mast cell activation involved in immune response / negative regulation of B cell receptor signaling pathway / regulation of B cell differentiation / epididymis development / CD27 signaling pathway / negative regulation of inflammatory response to wounding / transmembrane receptor protein tyrosine phosphatase activity / phosphorylation-dependent protein binding / Co-inhibition by BTLA ...negative regulation of humoral immune response mediated by circulating immunoglobulin / negative regulation of mast cell activation involved in immune response / negative regulation of B cell receptor signaling pathway / regulation of B cell differentiation / epididymis development / CD27 signaling pathway / negative regulation of inflammatory response to wounding / transmembrane receptor protein tyrosine phosphatase activity / phosphorylation-dependent protein binding / Co-inhibition by BTLA / negative regulation of lipopolysaccharide-mediated signaling pathway / negative regulation of neutrophil activation / CD22 mediated BCR regulation / alpha-beta T cell receptor complex / regulation of release of sequestered calcium ion into cytosol / Interleukin-37 signaling / positive regulation of cell adhesion mediated by integrin / Signal regulatory protein family interactions / platelet formation / Regulation of KIT signaling / megakaryocyte development / Signaling by ALK / negative regulation of T cell receptor signaling pathway / natural killer cell mediated cytotoxicity / Platelet sensitization by LDL / regulation of type I interferon-mediated signaling pathway / PECAM1 interactions / regulation of G1/S transition of mitotic cell cycle / negative regulation of interleukin-6 production / Regulation of IFNA/IFNB signaling / peptidyl-tyrosine dephosphorylation / negative regulation of tumor necrosis factor production / Interleukin-3, Interleukin-5 and GM-CSF signaling / Co-inhibition by PD-1 / Interleukin receptor SHC signaling / negative regulation of MAPK cascade / Regulation of IFNG signaling / hematopoietic progenitor cell differentiation / Growth hormone receptor signaling / regulation of ERK1 and ERK2 cascade / T cell proliferation / Nuclear events stimulated by ALK signaling in cancer / protein dephosphorylation / GPVI-mediated activation cascade / cell adhesion molecule binding / negative regulation of T cell proliferation / T cell costimulation / phosphotyrosine residue binding / protein tyrosine phosphatase activity / protein-tyrosine-phosphatase / protein tyrosine phosphatase activity, metal-dependent / histone H2AXY142 phosphatase activity / non-membrane spanning protein tyrosine phosphatase activity / SH2 domain binding / negative regulation of innate immune response / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / T cell activation / negative regulation of angiogenesis / B cell receptor signaling pathway / cytokine-mediated signaling pathway / peptidyl-tyrosine phosphorylation / SH3 domain binding / platelet aggregation / specific granule lumen / Interferon gamma signaling / Interferon alpha/beta signaling / MAPK cascade / tertiary granule lumen / cell-cell junction / mitotic cell cycle / T cell receptor signaling pathway / regulation of apoptotic process / cell differentiation / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / G protein-coupled receptor signaling pathway / negative regulation of cell population proliferation / positive regulation of cell population proliferation / Neutrophil degranulation / protein kinase binding / nucleolus / SARS-CoV-2 activates/modulates innate and adaptive immune responses / protein-containing complex / extracellular exosome / extracellular region / nucleoplasm / nucleus / membrane / plasma membrane / cytosol / cytoplasm
Similarity search - Function
: / Protein-tyrosine phosphatase, non-receptor type-6, -11 / SH2 domain / SHC Adaptor Protein / Protein tyrosine phosphatase superfamily / Protein-Tyrosine Phosphatase; Chain A / Protein tyrosine phosphatase, catalytic domain / PTP type protein phosphatase domain profile. / Protein-tyrosine phosphatase / Tyrosine-specific protein phosphatase, PTPase domain ...: / Protein-tyrosine phosphatase, non-receptor type-6, -11 / SH2 domain / SHC Adaptor Protein / Protein tyrosine phosphatase superfamily / Protein-Tyrosine Phosphatase; Chain A / Protein tyrosine phosphatase, catalytic domain / PTP type protein phosphatase domain profile. / Protein-tyrosine phosphatase / Tyrosine-specific protein phosphatase, PTPase domain / Protein-tyrosine phosphatase, catalytic / Protein tyrosine phosphatase, catalytic domain motif / Tyrosine specific protein phosphatases active site. / Protein-tyrosine phosphatase, active site / Tyrosine-specific protein phosphatases domain / Tyrosine specific protein phosphatases domain profile. / Protein-tyrosine phosphatase-like / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / SH2 domain superfamily / Alpha-Beta Complex / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Tyrosine-protein phosphatase non-receptor type 6
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å
AuthorsYang, J. / Liu, L. / He, D. / Song, X. / Liang, X. / Zhao, Z.J. / Zhou, G.W.
CitationJournal: J.Biol.Chem. / Year: 2003
Title: Crystal structure of human protein-tyrosine phosphatase SHP-1.
Authors: Yang, J. / Liu, L. / He, D. / Song, X. / Liang, X. / Zhao, Z.J. / Zhou, G.W.
History
DepositionSep 20, 2005Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 25, 2005Provider: repository / Type: Initial release
Revision 1.1May 1, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 14, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Tyrosine-protein phosphatase, non-receptor type 6


Theoretical massNumber of molelcules
Total (without water)60,3511
Polymers60,3511
Non-polymers00
Water34219
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)44.736, 100.404, 149.475
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein Tyrosine-protein phosphatase, non-receptor type 6 / Protein-tyrosine phosphatase 1C / PTP-1C / Hematopoietic cell protein-tyrosine phosphatase / SH- ...Protein-tyrosine phosphatase 1C / PTP-1C / Hematopoietic cell protein-tyrosine phosphatase / SH-PTP1 / Protein-tyrosine phosphatase SHP-1


Mass: 60350.727 Da / Num. of mol.: 1 / Fragment: SHP-1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PTPN6, HCP, PTP1C / Production host: Escherichia coli (E. coli) / References: UniProt: P29350, protein-tyrosine-phosphatase
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 19 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.78 Å3/Da / Density % sol: 55.49 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7
Details: PEG 800, DTT, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.978 Å
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.978 Å / Relative weight: 1
ReflectionResolution: 2.8→40 Å / Num. obs: 17214 / % possible obs: 94.8 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.8 % / Rmerge(I) obs: 0.099 / Rsym value: 0.099 / Net I/σ(I): 5.1
Reflection shellResolution: 2.8→2.95 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.315 / Mean I/σ(I) obs: 2 / Num. unique all: 2210 / Rsym value: 0.315 / % possible all: 89.9

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Processing

Software
NameVersionClassification
DENZOdata reduction
SCALAdata scaling
AMoREphasing
CNSrefinement
CCP4(SCALA)data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→20 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
RfactorNum. reflectionSelection details
Rfree0.296 1375 10% of observed reflections
Rwork0.233 --
all0.25 --
obs0.241 16788 -
Refinement stepCycle: LAST / Resolution: 2.8→20 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4043 0 0 19 4062

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