ENGINEERED MUTATION IN CHAIN A, SER 954 TO ALA ENGINEERED MUTATION IN CHAIN B, SER 954 TO ALA ...ENGINEERED MUTATION IN CHAIN A, SER 954 TO ALA ENGINEERED MUTATION IN CHAIN B, SER 954 TO ALA CATALYTIC ACTIVITY: ENDOHYDROLYSIS OF 1,4-BETA-D-XYLOSIDIC LINKAGES IN XYLANS.
配列の詳細
ONLY THE FERULOYL ESTERASE DOMAIN WAS SUBCLONED. S954A MUTANT. THE SEQUENCE CONFLICTS (RESIDUES ...ONLY THE FERULOYL ESTERASE DOMAIN WAS SUBCLONED. S954A MUTANT. THE SEQUENCE CONFLICTS (RESIDUES 1017 AND 1018) ARE DUE TO ERRORS IN THE UNIPROT ENTRY FROM THE ORIGINAL SEQUENCING OF THE PROTEIN.
-
実験情報
-
実験
実験
手法: X線回折 / 使用した結晶の数: 1
-
試料調製
結晶
マシュー密度: 2.95 Å3/Da / 溶媒含有率: 58 %
結晶化
pH: 7.5 詳細: NA ACETATE 1M CD ACETATE 50 MM GLYCEROL 5% HEPES PH 7.5 100 MM
解像度: 1.4→79.06 Å / Cor.coef. Fo:Fc: 0.981 / Cor.coef. Fo:Fc free: 0.974 / SU B: 0.477 / SU ML: 0.019 / 交差検証法: THROUGHOUT / ESU R: 0.039 / ESU R Free: 0.038 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
反射数
%反射
Selection details
Rfree
0.134
7700
5 %
RANDOM
Rwork
0.111
-
-
-
obs
0.113
146352
97.8 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK