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- PDB-1sbh: SUBTILISIN BPN' 8397+1 (E.C. 3.4.21.14) MUTANT (M50F, N76D, G169A... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1sbh | ||||||
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Title | SUBTILISIN BPN' 8397+1 (E.C. 3.4.21.14) MUTANT (M50F, N76D, G169A, Q206C, N218S, K256Y) | ||||||
![]() | SUBTILISIN 8397+1 | ||||||
![]() | HYDROLASE (SERINE PROTEASE) | ||||||
Function / homology | ![]() subtilisin / sporulation resulting in formation of a cellular spore / fibrinolysis / serine-type endopeptidase activity / proteolysis / extracellular space / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Kidd, R.D. / Farber, G.K. | ||||||
![]() | Journal: J.Am.Chem.Soc. / Year: 1996 Title: A weak calcium binding site in subtilisin BPN' has a dramatic effect on protein stability. Authors: Kidd, R.D. / Yennawar, H.P. / Sears, P. / Wong, C.H. / Farber, G.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 58.8 KB | Display | ![]() |
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PDB format | ![]() | 45.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 411.5 KB | Display | ![]() |
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Full document | ![]() | 413.3 KB | Display | |
Data in XML | ![]() | 12.1 KB | Display | |
Data in CIF | ![]() | 16.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 168 |
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Components
#1: Protein | Mass: 27581.498 Da / Num. of mol.: 1 / Mutation: M50F, N76D, G169A, Q206C, N218S, K256Y Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.45 % | |||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 39.5 % | |||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.6 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.54 |
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Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jul 10, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→17 Å / Num. obs: 21228 / % possible obs: 100 % / Redundancy: 1 % / Rmerge(I) obs: 0.113 |
Reflection | *PLUS Rmerge(I) obs: 0.113 |
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Processing
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Refinement | Resolution: 1.8→17 Å / σ(F): 2
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Displacement parameters | Biso mean: 20.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→17 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |