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Open data
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Basic information
Entry | Database: PDB / ID: 1sbt | |||||||||
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Title | ATOMIC COORDINATES FOR SUBTILISIN BPN (OR NOVO) | |||||||||
![]() | SUBTILISIN BPN' | |||||||||
![]() | HYDROLASE (SERINE PROTEINASE) | |||||||||
Function / homology | ![]() subtilisin / sporulation resulting in formation of a cellular spore / fibrinolysis / serine-type endopeptidase activity / proteolysis / extracellular space / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Alden, R.A. / Birktoft, J.J. / Kraut, J. / Robertus, J.D. / Wright, C.S. | |||||||||
![]() | ![]() Title: Atomic coordinates for subtilisin BPN' (or Novo). Authors: Alden, R.A. / Birktoft, J.J. / Kraut, J. / Robertus, J.D. / Wright, C.S. #1: ![]() Title: Polypeptide Halomethyl Ketones Bind to Serine Proteases as Analogs of the Tetrahedral Intermediate,X-Ray Crystallographic Comparison of Lysine-and Phenylalanine-Polypeptide Chloromethyl Ketone-Inhibited Subtilisin Authors: Poulos, T.L. / Alden, R.A. / Freer, S.T. / Birktoft, J.J. / Kraut, J. #2: ![]() Title: X-Ray Crystallographic Study of Boronic Acid Adducts with Subtilisin Bpn(Novo),A Model for the Catalytic Transition State Authors: Matthews, D.A. / Alden, R.A. / Birktoft, J.J. / Freer, S.T. / Kraut, J. #3: ![]() Title: Subtilisin,A Stereochemical Mechanism Involving Transition-State Stabilization Authors: Robertus, J.D. / Kraut, J. / Alden, R.A. / Birktoft, J.J. #4: ![]() Title: An X-Ray Crystallographic Study of the Binding of Peptide Chloromethyl Ketone Inhibitors to Subtilisin Bpn Authors: Robertus, J.D. / Alden, R.A. / Birktoft, J.J. / Kraut, J. / Powers, J.C. / Wilcox, P.E. #5: ![]() Title: The Aromatic Substrate Binding Site in Subtilisin Bpnand its Resemblance to Chymotrypsin Authors: Kraut, J. / Robertus, J.D. / Birktoft, J.J. / Alden, R.A. / Wilcox, P.E. / Powers, J.C. #6: ![]() Title: On the Identity of Subtilisins Bpnand Novo Authors: Robertus, J.D. / Alden, R.A. / Kraut, J. #7: ![]() Title: A Hydrogen-Bond Network at the Active Site of Subtilisin Bpn Authors: Alden, R.A. / Wright, C.S. / Kraut, J. #8: ![]() Title: Structure of Subtilisin Bpnat 2.5 Angstroms Resolution Authors: Wright, C.S. / Alden, R.A. / Kraut, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 55.1 KB | Display | ![]() |
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PDB format | ![]() | 32.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 373.6 KB | Display | ![]() |
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Full document | ![]() | 474.9 KB | Display | |
Data in XML | ![]() | 20.3 KB | Display | |
Data in CIF | ![]() | 26.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: THE FO-FC MAP INDICATES COORDINATE ERRORS OF MORE THAN 1 ANGSTROM. 2: THESE COORDINATES ARE THE OUTPUT OF A REFINEMENT BY DIAMOND'S MODEL-BUILDING PROGRAM. |
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Components
#1: Protein | Mass: 27552.525 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.54 % |
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Crystal grow | *PLUS Method: other / Details: Wright, C.S., (1969) Nature, 221, 235. |
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Processing
Refinement | Highest resolution: 2.5 Å Details: THE FO-FC MAP INDICATES COORDINATE ERRORS OF MORE THAN 1 ANGSTROM. THESE COORDINATES ARE THE OUTPUT OF A REFINEMENT BY DIAMOND'S MODEL-BUILDING PROGRAM | ||||||||||||
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Refinement step | Cycle: LAST / Highest resolution: 2.5 Å
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