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- PDB-1rtq: The 0.95 Angstrom Resolution Crystal Structure of the Aminopeptid... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1rtq | ||||||
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Title | The 0.95 Angstrom Resolution Crystal Structure of the Aminopeptidase from Aeromonas proteolytica | ||||||
![]() | Bacterial leucyl aminopeptidase | ||||||
![]() | HYDROLASE / Bimetallic / Zinc / High Resolution / Aminopeptidase | ||||||
Function / homology | ![]() bacterial leucyl aminopeptidase / metalloexopeptidase activity / aminopeptidase activity / proteolysis / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Desmarais, W. / Bienvenue, D.L. / Krzysztof, B.P. / Holz, R.C. / Petsko, G.A. / Ringe, D. | ||||||
![]() | ![]() Title: The high-resolution structures of the neutral and the low pH crystals of aminopeptidase from Aeromonas proteolytica. Authors: Desmarais, W. / Bienvenue, D.L. / Bzymek, K.P. / Petsko, G.A. / Ringe, D. / Holz, R.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 175.9 KB | Display | ![]() |
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PDB format | ![]() | 149.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 32238.150 Da / Num. of mol.: 1 / Fragment: Aminopeptidase Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q01693, bacterial leucyl aminopeptidase | ||||||
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#2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.22 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: HEPES, Potassium Thiocyanate, Sodium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: Schalk, C., (1992) Arch. Biochem. Biophys., 294, 91. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 1, 1998 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 0.95→30 Å / Num. all: 191127 / Num. obs: 191127 / % possible obs: 95 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 0.95→0.98 Å / % possible all: 59 |
Reflection | *PLUS Num. measured all: 3348266 / Rmerge(I) obs: 0.064 |
Reflection shell | *PLUS % possible obs: 59 % / Rmerge(I) obs: 0.23 / Mean I/σ(I) obs: 2.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 0.95→30 Å
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Software | *PLUS Name: SHELXL / Version: 97 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 27 Å | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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