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- PDB-1rqs: NMR structure of C-terminal domain of ribosomal protein L7 from E.coli -
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Open data
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Basic information
Entry | Database: PDB / ID: 1rqs | ||||||
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Title | NMR structure of C-terminal domain of ribosomal protein L7 from E.coli | ||||||
![]() | 50S ribosomal protein L7/L12 | ||||||
![]() | RIBOSOME / protein L7/L12 | ||||||
Function / homology | ![]() large ribosomal subunit / ribosome binding / cytosolic large ribosomal subunit / cytoplasmic translation / structural constituent of ribosome / translation / mRNA binding / protein homodimerization activity / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics in torsion angle space | ||||||
![]() | Bocharov, E.V. / Sobol, A.G. / Pavlov, K.V. / Korzhnev, D.M. / Jaravine, V.A. / Gudkov, A.T. / Arseniev, A.S. | ||||||
![]() | ![]() Title: From structure and dynamics of protein L7/L12 to molecular switching in ribosome. Authors: Bocharov, E.V. / Sobol, A.G. / Pavlov, K.V. / Korzhnev, D.M. / Jaravine, V.A. / Gudkov, A.T. / Arseniev, A.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 419.9 KB | Display | ![]() |
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PDB format | ![]() | 352.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 334.8 KB | Display | ![]() |
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Full document | ![]() | 437.4 KB | Display | |
Data in XML | ![]() | 21.1 KB | Display | |
Data in CIF | ![]() | 35.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1rqtC ![]() 1rquC ![]() 1rqvC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 7573.641 Da / Num. of mol.: 1 / Fragment: C-Terminal Domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli, Escherichia coli O6, Escherichia coli O157:H7, Shigella flexneri Genus: Escherichia, Escherichia, Escherichia, Shigella / Species: , Escherichia coli, Escherichia coli, / Strain: , O6, O157:H7, / Plasmid: pPR1 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
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Sample preparation
Details |
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Sample conditions | Ionic strength: 0.15 / pH: 6.9 / Pressure: ambient / Temperature: 303 K | ||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics in torsion angle space Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,target function Conformers calculated total number: 200 / Conformers submitted total number: 20 |