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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1nf1 | ||||||
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タイトル | THE GAP RELATED DOMAIN OF NEUROFIBROMIN | ||||||
![]() | PROTEIN (NEUROFIBROMIN) | ||||||
![]() | SIGNALING PROTEIN / NEUROFIBROMIN / TYPE I NEUROFIBROMATOSIS / NF1 / RAS / GAP / SIGNAL TRANSDUCTION / CANCER / GROWTH REGULATION / GTP HYDROLYSIS / PATIENT MUTATION / ARGININE FINGER | ||||||
機能・相同性 | ![]() positive regulation of mast cell apoptotic process / negative regulation of Rac protein signal transduction / regulation of glial cell differentiation / observational learning / Schwann cell migration / negative regulation of Schwann cell migration / vascular associated smooth muscle cell migration / amygdala development / gamma-aminobutyric acid secretion, neurotransmission / negative regulation of mast cell proliferation ...positive regulation of mast cell apoptotic process / negative regulation of Rac protein signal transduction / regulation of glial cell differentiation / observational learning / Schwann cell migration / negative regulation of Schwann cell migration / vascular associated smooth muscle cell migration / amygdala development / gamma-aminobutyric acid secretion, neurotransmission / negative regulation of mast cell proliferation / mast cell apoptotic process / Schwann cell proliferation / vascular associated smooth muscle cell proliferation / mast cell proliferation / glutamate secretion, neurotransmission / negative regulation of Schwann cell proliferation / negative regulation of leukocyte migration / negative regulation of vascular associated smooth muscle cell migration / positive regulation of adenylate cyclase activity / regulation of cell-matrix adhesion / forebrain morphogenesis / negative regulation of neurotransmitter secretion / hair follicle maturation / cell communication / regulation of blood vessel endothelial cell migration / smooth muscle tissue development / camera-type eye morphogenesis / negative regulation of oligodendrocyte differentiation / sympathetic nervous system development / myelination in peripheral nervous system / myeloid leukocyte migration / peripheral nervous system development / phosphatidylcholine binding / metanephros development / phosphatidylethanolamine binding / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / negative regulation of Ras protein signal transduction / collagen fibril organization / regulation of bone resorption / regulation of long-term synaptic potentiation / neural tube development / endothelial cell proliferation / forebrain astrocyte development / artery morphogenesis / regulation of postsynapse organization / regulation of synaptic transmission, GABAergic / negative regulation of neuroblast proliferation / negative regulation of MAPK cascade / adrenal gland development / negative regulation of protein import into nucleus / pigmentation / negative regulation of cell-matrix adhesion / spinal cord development / regulation of GTPase activity / Rac protein signal transduction / oligodendrocyte differentiation / negative regulation of osteoclast differentiation / negative regulation of endothelial cell proliferation / RAS signaling downstream of NF1 loss-of-function variants / negative regulation of astrocyte differentiation / extrinsic apoptotic signaling pathway via death domain receptors / neuroblast proliferation / regulation of angiogenesis / Schwann cell development / negative regulation of stem cell proliferation / negative regulation of fibroblast proliferation / skeletal muscle tissue development / extrinsic apoptotic signaling pathway in absence of ligand / positive regulation of vascular associated smooth muscle cell proliferation / positive regulation of endothelial cell proliferation / extracellular matrix organization / negative regulation of angiogenesis / negative regulation of cell migration / phosphatidylinositol 3-kinase/protein kinase B signal transduction / GTPase activator activity / regulation of ERK1 and ERK2 cascade / osteoclast differentiation / positive regulation of GTPase activity / liver development / negative regulation of MAP kinase activity / stem cell proliferation / long-term synaptic potentiation / regulation of long-term neuronal synaptic plasticity / negative regulation of protein kinase activity / wound healing / visual learning / brain development / cerebral cortex development / cognition / Regulation of RAS by GAPs / osteoblast differentiation / protein import into nucleus / MAPK cascade / positive regulation of neuron apoptotic process / presynapse / heart development / cellular response to heat / fibroblast proliferation / actin cytoskeleton organization / regulation of gene expression 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Scheffzek, K. / Ahmadian, M.R. / Wiesmueller, L. / Kabsch, W. / Stege, P. / Schmitz, F. / Wittinghofer, A. | ||||||
![]() | ![]() タイトル: Structural analysis of the GAP-related domain from neurofibromin and its implications. 著者: Scheffzek, K. / Ahmadian, M.R. / Wiesmuller, L. / Kabsch, W. / Stege, P. / Schmitz, F. / Wittinghofer, A. #1: ![]() タイトル: The Ras-Rasgap Complex: Structural Basis for Gtpase Activation and its Loss in Oncogenic Ras Mutants 著者: Scheffzek, K. / Ahmadian, M.R. / Kabsch, W. / Wiesmueller, L. / Lautwein, A. / Schmitz, F. / Wittinghofer, A. #2: ![]() タイトル: Confirmation of the Arginine-Finger Hypothesis for the Gap-Stimulated GTP- Hydrolysis Reaction of Ras 著者: Ahmadian, M.R. / Stege, P. / Scheffzek, K. / Wittinghofer, A. #3: ![]() タイトル: Structural Differences in the Minimal Catalytic Domains of the Gtpasse- Activating Proteins P120Gap and Neurofibromin 著者: Ahmadian, M.R. / Wiesmueller, L. / Lautwein, A. / Bischoff, F.R. / Wittinghofer, A. #4: ![]() タイトル: 3-Dimensional Structure of the Gtpase Activating Domain of Human P120Gap and Implications for the Interaction with Ras 著者: Scheffzek, K. / Lautwein, A. / Kabsch, W. / Ahmadian, M.R. / Wittinghofer, A. #5: ![]() タイトル: Formation of a Transition-State Analog of the Ras Gtpase Reaction by Ras:Gdp, Tetrafluoroaluminate and Gtpase-Activating Proteins 著者: Mittal, R. / Ahmadian, M.R. / Goody, R.S. / Wittinghofer, A. #6: ![]() タイトル: The Neurofibromatosis Type I Gene and its Protein Product 著者: Gutmann, D.H. / Collins, F.S. #7: ![]() タイトル: The NF1 Locus Encodes a Protein Functionally Related to Mammalian Gap and Yeast Ira Proteins 著者: Ballester, R. / Marchuk, D. / Boguski, M. / Saulino, A. / Letcher, R. / Wigler, M. / Collins, F. #8: ![]() タイトル: The Gap-Related Domain of the Neurofibromatosis Type I Gene Product Interacts with Ras P21 著者: Martin, G.A. / Viskochil, D. / Bollag, G. / Mccabe, P.C. / Crosier, W.J. / Hausbruck, H. / Conroy, L. / Clark, R. / O'Connell, P. / Cawthon, R.M. / Innis, M.A. / Mccormick, F. #9: ![]() タイトル: The Catalytic Domain of the Neurofibromatosis Type I Gene Product Stimulates Ras Gtpase and Complements Ira Mutants of S. Cerevisiae 著者: Xu, G. / Lin, B. / Tanaka, K. / Dunn, D. / Wod, D. / Gesteland, R. / White, R. / Weiss, R. / Tamanoi, F. #10: ![]() タイトル: Refined Crystal Structure of the Triphosphate Conformation of H-Ras P21 at 1.35 A Resolution: Implications for the Mechanism of GTP Hydrolysis 著者: Pai, E.F. / Krengel, U. / Petsko, G.A. / Goody, R.S. / Kabsch, W. / Wittinghofer, A. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 64.6 KB | 表示 | ![]() |
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PDB形式 | ![]() | 46.8 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 367.9 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 374.2 KB | 表示 | |
XML形式データ | ![]() | 7.1 KB | 表示 | |
CIF形式データ | ![]() | 10.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 37982.672 Da / 分子数: 1 / 断片: GAP RELATED DOMAIN / 由来タイプ: 組換発現 / 詳細: SEE REF.5 FOR DETAILS / 由来: (組換発現) ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.24 Å3/Da / 溶媒含有率: 44.98 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | pH: 8 / 詳細: S. REF. DESCRIBING THE STRUCTURE, pH 8 | ||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS pH: 7.5 / 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 277 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 1997年2月26日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.91 Å / 相対比: 1 |
反射 | 解像度: 2.5→100 Å / Num. obs: 50172 / % possible obs: 99 % / 冗長度: 4.3 % / Biso Wilson estimate: 52.4 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 11.6 |
反射 シェル | 解像度: 2.5→2.6 Å / 冗長度: 3.1 % / Rmerge(I) obs: 0.3 / Mean I/σ(I) obs: 3.6 / % possible all: 99.8 |
反射 | *PLUS Num. obs: 11665 / % possible obs: 99.8 % / Num. measured all: 50172 / Rmerge(I) obs: 0.073 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]()
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原子変位パラメータ | Biso mean: 47.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.5→30 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.5→2.61 Å / Rfactor Rfree error: 0.036 / Total num. of bins used: 8
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ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS 最高解像度: 2.5 Å / 最低解像度: 30 Å / σ(F): 2 / % reflection Rfree: 10 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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LS精密化 シェル | *PLUS 最高解像度: 2.5 Å / Rfactor Rfree: 0.436 / % reflection Rfree: 11.9 % / Rfactor Rwork: 0.4 |