[English] 日本語
![](img/lk-miru.gif)
- PDB-1hg7: High resolution structure of HPLC-12 type III antifreeze protein ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1hg7 | ||||||
---|---|---|---|---|---|---|---|
Title | High resolution structure of HPLC-12 type III antifreeze protein from Ocean Pout Macrozoarces americanus | ||||||
![]() | HPLC-12 TYPE III ANTIFREEZE PROTEIN | ||||||
![]() | ANTIFREEZE PROTEIN / OCEAN POUT / MACROZOARCES AMERICANUS / ICE-BINDING PROTEIN | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Antson, A.A. / Smith, D.J. / Roper, D.I. / Lewis, S. / Caves, L.S.D. / Verma, C.S. / Buckley, S.L. / Lillford, P.J. / Hubbard, R.E. | ||||||
![]() | ![]() Title: Understanding the Mechanism of Ice Binding by Type III Antifreeze Protein Authors: Antson, A.A. / Smith, D.J. / Roper, D.I. / Lewis, S. / Caves, L.S.D. / Verma, C.S. / Buckley, S.L. / Lillford, P.J. / Hubbard, R.E. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 46.4 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 32.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 410.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 410.8 KB | Display | |
Data in XML | ![]() | 7.1 KB | Display | |
Data in CIF | ![]() | 9.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1gziS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 7039.351 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene (production host): RECOMBINANT TYPE III AFP HPURCE 10 FRACTION 12 Production host: ![]() ![]() |
---|---|
#2: Chemical | ChemComp-SO4 / |
#3: Water | ChemComp-HOH / |
Compound details | CHAIN A ENGINEERED MUTATION PRO64ALA, PRO65ALA, ALA66 DELETION MUTATNT ANTIFREEZE PROTEINS LOWER ...CHAIN A ENGINEERED |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 39.8 % | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 4.6 / Details: 50% AMMONIUM SULFATE, 0.1M SODIUM ACETATE, PH 4.6 | ||||||||||||||||||||
Crystal | *PLUS Density % sol: 40.2 % | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, hanging drop / PH range low: 5 / PH range high: 4.6 | ||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 110 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 15, 1996 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91 Å / Relative weight: 1 |
Reflection | Resolution: 1.15→45 Å / Num. obs: 20883 / % possible obs: 99.9 % / Observed criterion σ(I): 0 / Redundancy: 6.7 % / Rmerge(I) obs: 0.108 / Net I/σ(I): 5.2 |
Reflection shell | Resolution: 1.15→1.17 Å / Redundancy: 5 % / Rmerge(I) obs: 0.493 / Mean I/σ(I) obs: 3.7 / % possible all: 100 |
Reflection | *PLUS Lowest resolution: 45 Å |
Reflection shell | *PLUS % possible obs: 100 % |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1GZI Resolution: 1.15→40 Å / σ(F): 0
| |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.15→40 Å
| |||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||
Software | *PLUS Name: SHELXL / Classification: refinement | |||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 4 / Num. reflection Rfree: 2066 / Rfactor all: 0.12 / Rfactor obs: 0.104 / Rfactor Rwork: 0.12 | |||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |