+Open data
-Basic information
Entry | Database: PDB / ID: 6ff1 | |||||||||
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Title | CsoZ metallochaperone | |||||||||
Components | CsoZ | |||||||||
Keywords | CHAPERONE / metallochaperone / copper / copper homeostasis / staphylococcus aureus | |||||||||
Function / homology | Heavy metal-associated domain superfamily / Heavy-metal-associated domain profile. / Heavy metal-associated domain, HMA / metal ion binding / HMA domain-containing protein Function and homology information | |||||||||
Biological species | Staphylococcus aureus (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.3 Å | |||||||||
Authors | Pelicioli-Riboldi, G. / Basle, A. / Waldron, K. | |||||||||
Funding support | United Kingdom, Brazil, 2items
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Citation | Journal: To Be Published Title: The copper homeostasis system of Staphylococcus aureus: Two putative metallochaperones with contrasting copper binding properties. Authors: Pelicioli-Riboldi, G. / Tarrant, E. / Basle, A. / Davies, O. / Waldron, K. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6ff1.cif.gz | 44.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6ff1.ent.gz | 33.2 KB | Display | PDB format |
PDBx/mmJSON format | 6ff1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ff/6ff1 ftp://data.pdbj.org/pub/pdb/validation_reports/ff/6ff1 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 7848.718 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (strain NCTC 8325) (bacteria) Strain: SH1000 / Gene: SAOUHSC_02321 / Production host: Escherichia coli (E. coli) / References: UniProt: Q2FWH0 |
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#2: Chemical | ChemComp-CA / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.11 Å3/Da / Density % sol: 60.49 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 200 mM CaCl2, 100 mM sodium acetate pH 5.0, 20%w/v PEG 6000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9794 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 12, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 1.3→44.21 Å / Num. obs: 24651 / % possible obs: 100 % / Redundancy: 9.8 % / CC1/2: 0.997 / Net I/σ(I): 10 |
Reflection shell | Resolution: 1.3→1.32 Å / Redundancy: 8.4 % / Mean I/σ(I) obs: 0.9 / Num. unique obs: 1184 / CC1/2: 0.868 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 1.3→44.21 Å / Cor.coef. Fo:Fc: 0.977 / Cor.coef. Fo:Fc free: 0.967 / SU B: 1.625 / SU ML: 0.029 / Cross valid method: THROUGHOUT / ESU R: 0.037 / ESU R Free: 0.039 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.051 Å2
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Refinement step | Cycle: 1 / Resolution: 1.3→44.21 Å
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Refine LS restraints |
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