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Yorodumi- PDB-1kdf: NORTH-ATLANTIC OCEAN POUT ANTIFREEZE PROTEIN TYPE III ISOFORM HPL... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1kdf | ||||||
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Title | NORTH-ATLANTIC OCEAN POUT ANTIFREEZE PROTEIN TYPE III ISOFORM HPLC12 MUTANT, NMR, MINIMIZED AVERAGE STRUCTURE | ||||||
Components | ANTIFREEZE PROTEIN | ||||||
Keywords | ANTIFREEZE PROTEIN / ICE BINDING PROTEIN / THERMAL HYSTERESIS PROTEIN / GLYCOPROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Macrozoarces americanus (ocean pout) | ||||||
Method | SOLUTION NMR / DG_SUB_EMBED, DGSA, REFINE | ||||||
Authors | Sonnichsen, F.D. / Deluca, C.I. / Davies, P.L. / Sykes, B.D. | ||||||
Citation | Journal: Structure / Year: 1996 Title: Refined solution structure of type III antifreeze protein: hydrophobic groups may be involved in the energetics of the protein-ice interaction. Authors: Sonnichsen, F.D. / DeLuca, C.I. / Davies, P.L. / Sykes, B.D. #1: Journal: Protein Sci. / Year: 1994 Title: Structure-Function Relationship in the Globular Type III Antifreeze Protein: Identification of a Cluster of Surface Residues Required for Binding to Ice Authors: Chao, H. / Sonnichsen, F.D. / Deluca, C.I. / Sykes, B.D. / Davies, P.L. #2: Journal: Science / Year: 1993 Title: The Nonhelical Structure of Antifreeze Protein Type III Authors: Sonnichsen, F.D. / Sykes, B.D. / Chao, H. / Davies, P.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1kdf.cif.gz | 19.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1kdf.ent.gz | 11.3 KB | Display | PDB format |
PDBx/mmJSON format | 1kdf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1kdf_validation.pdf.gz | 246.4 KB | Display | wwPDB validaton report |
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Full document | 1kdf_full_validation.pdf.gz | 246.2 KB | Display | |
Data in XML | 1kdf_validation.xml.gz | 2.6 KB | Display | |
Data in CIF | 1kdf_validation.cif.gz | 3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kd/1kdf ftp://data.pdbj.org/pub/pdb/validation_reports/kd/1kdf | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7525.890 Da / Num. of mol.: 1 / Mutation: INS(M0), P64A, P65A, INS(K66, D67, E68, L69) Source method: isolated from a genetically manipulated source Details: TYPE III ISOFORM HPLC 12, QAE-COMPONENT / Source: (gene. exp.) Macrozoarces americanus (ocean pout) / Tissue: BLOOD / Gene: K38 / Variant: HPLC-12 / Plasmid: JM83 / Gene (production host): K38 / Production host: Escherichia coli (E. coli) / References: UniProt: P19614 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 7.6 / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 600 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: DG_SUB_EMBED, DGSA, REFINE / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: AVERAGE / Conformers calculated total number: 50 / Conformers submitted total number: 1 |